Skin‐derived precursor schwann cell myelination capacity in focal tibial demyelination

Grochmal, Joey; Dhaliwal, S.K.; Stys, Peter K.; Minnen, Jan van; Midha, Rajiv

doi:10.1002/mus.24136

Cited by 21 publications

(15 citation statements)

References 27 publications

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“…These diverse multipotent MSCs can also be differentiated into cells of the ectodermal lineage including neurons and glial cells. Various studies using skin-derived MSCs, adipose tissue-derived MSCs (AMSCs), bone marrow-derived MSCs, and umbilical cord-derived MSCs have been performed in attempts to differentiate them into SCs under diverse conditions [9,10,11,12]. Although bone marrow-derived MSCs are one of the most intensively studied types of MSC associated with SC differentiation, they are limited in terms of clinical applications because of their low yields and the need for invasive procedures to isolate them [13].…”

Section: Introductionmentioning

confidence: 99%

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Jung

Park

Choi

et al. 2016

IJMS

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Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been performed and has shown promising clinical results for treating nerve injuries and donor site morbidity, and insufficient production of the cells have been considered as a major issue. Here, we performed differentiation of tonsil-derived mesenchymal stem cells (T-MSCs) into SC-like cells (T-MSC-SCs), to evaluate T-MSC-SCs as an alternative to SCs. Using SC markers such as CAD19, GFAP, MBP, NGFR, S100B, and KROX20 during quantitative real-time PCR we detected the upregulation of NGFR, S100B, and KROX20 and the downregulation of CAD19 and MBP at the fully differentiated stage. Furthermore, we found myelination of axons when differentiated SCs were cocultured with mouse dorsal root ganglion neurons. The application of T-MSC-SCs to a mouse model of sciatic nerve injury produced marked improvements in gait and promoted regeneration of damaged nerves. Thus, the transplantation of human T-MSCs might be suitable for assisting in peripheral nerve regeneration.

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Section: Introductionmentioning

confidence: 99%

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Jung

Park

Choi

et al. 2016

IJMS

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“…Little is known regarding the role of SKPs in wound healing, but several studies have shown that SKP‐derived Schwann cells may help in promoting peripheral nerve regeneration . SKP‐derived Schwann cells or SKPs themselves are, in a model of nerve injury, capable of inducing remyelination and regeneration of peripheral nerves . Indeed, without predifferentiation, grafted proliferative SKPs induce positive effects on cutaneous nerve regeneration.…”

Section: Therapeutic Strategies To Improve Wound Healing and Reinnervmentioning

confidence: 98%

New insights into the roles of myofibroblasts and innervation during skin healing and innovative therapies to improve scar innervation

Lebonvallet

Laverdet

Miséry

et al. 2018

Experimental Dermatology

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During the resolution phase of normal skin wound healing, there is a considerable loss of various cell types, including myofibroblasts by apoptosis. Inappropriate delay of apoptosis, and thus increased survival of myofibroblasts, may be a factor leading to pathologies and excessive scarring. Considerable data now clearly suggest that innervation plays a major role in wound healing, including the modulation of fibroblast cellular activity. An abnormal level of neuromediators is implicated not only in the development of chronic wounds but also in excessive scar formation.Understanding interactions between neuromediators and myofibroblasts, allowing normal reinnervation and having adequate levels of neuromediators during the healing process are clearly important to avoid the appearance of pathological healing or fibrosis/scarring. The aim of this review was first to discuss the mechanisms leading to normal or excessive scarring and then to present the roles of innervation during wound healing. Finally, the latest therapeutic strategies to help wound repair and reinnervation after skin damage will be introduced. Advantages and limitations in the use of neuropeptides, growth factors and biomaterials will be discussed as well as the most recent studies on electrostimulation and the potential of targeting resident skin mesenchymal stem cells. K E Y W O R D Sinnervation, myofibroblast, pathological scarring, skin repair, skin-derived precursor | 951LEBONVALLET ET AL.

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“…We have previously injected lentiviral enhanced blue fluorescent protein (BFP) SCs into Thy‐1 green fluorescent protein (GFP) rats that underwent doxorubicin‐induced focal myelination . Our group showed that these cells may aid regeneration through growth factor production, debris clearance, and myelination (Figure ).…”

Section: Skin‐derived Precursor Cellsmentioning

confidence: 99%

Stem‐cell–based therapies to enhance peripheral nerve regeneration

et al. 2019

Self Cite

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Peripheral nerve injury remains a major cause of morbidity in trauma patients. Despite advances in microsurgical techniques and improved understanding of nerve regeneration, obtaining satisfactory outcomes after peripheral nerve injury remains a difficult clinical problem. There is a growing body of evidence in preclinical animal studies demonstrating the supportive role of stem cells in peripheral nerve regeneration after injury. The characteristics of both mesoderm‐derived and ectoderm‐derived stem cell types and their role in peripheral nerve regeneration are discussed, specifically focusing on the presentation of both foundational laboratory studies and translational applications. The current state of clinical translation is presented, with an emphasis on both ethical considerations of using stems cells in humans and current governmental regulatory policies. Current advancements in cell‐based therapies represent a promising future with regard to supporting nerve regeneration and achieving significant functional recovery after debilitating nerve injuries.

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Skin‐derived precursor schwann cell myelination capacity in focal tibial demyelination

Abstract: SKP-SCs are robustly capable of myelination. They improve the recovery of a focal tibial nerve demyelination model by myelinating a measured percentage of axons.

Cited by 21 publications

References 27 publications

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

New insights into the roles of myofibroblasts and innervation during skin healing and innovative therapies to improve scar innervation

Stem‐cell–based therapies to enhance peripheral nerve regeneration

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