Skin-Derived Precursors as a Source of Progenitors for Corneal Endothelial Regeneration

Inagaki, Emi; Hatou, Shin; Higa, Kazunari; Yoshida, Shigeo; Shibata, Shinsuke; Ohta, Michio; Shimmura, Shigeto

doi:10.1002/sctm.16-0162

Cited by 41 publications

(31 citation statements)

References 39 publications

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“…Transplantation of cultured HCECs or possible precursor cells has been performed to overcome the shortage of donor tissue [59,[73][74][75][76]. Diverse research groups have identified markers for HCECs, including CD166, glypican 4 (GPC4), CD200, CD56, Integrin Subunit Alpha 3 (ITGA3), and CD49c [77][78][79][80][81].…”

Section: Endothelial Cell Density and Morphological Indicators As Gramentioning

confidence: 99%

Variable Responses to Corneal Grafts: Insights from Immunology and Systems Biology

Zazzo

Lee

Sung

et al. 2020

JCM

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Corneal grafts interact with their hosts via complex immunobiological processes that sometimes lead to graft failure. Prediction of graft failure is often a tedious task due to the genetic and nongenetic heterogeneity of patients. As in other areas of medicine, a reliable prediction method would impact therapeutic decision-making in corneal transplantation. Valuable insights into the clinically observed heterogeneity of host responses to corneal grafts have emerged from multidisciplinary approaches, including genomics analyses, mechanical studies, immunobiology, and theoretical modeling. Here, we review the emerging concepts, tools, and new biomarkers that may allow for the prediction of graft survival.

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Section: Endothelial Cell Density and Morphological Indicators As Gramentioning

confidence: 99%

Variable Responses to Corneal Grafts: Insights from Immunology and Systems Biology

Zazzo

Lee

Sung

et al. 2020

JCM

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“…Previously, Inagaki et al . 17 had acquired CEC-like cells from SKPs. However, there are some difference between our work and their work.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, Inagaki et al . 17 had reported corneal endothelial-like cells could be derived from SKPs. Compared to their work, we use different derivation method, operation method and animal model.…”

Section: Introductionmentioning

confidence: 99%

Therapy of corneal endothelial dysfunction with corneal endothelial cell-like cells derived from skin-derived precursors

Shen

Sun

Zhang

et al. 2017

Sci Rep

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Corneal endothelial dysfunction occurs when corneal endothelial cells (CECs) are dramatically lost and eventually results in vision loss. Corneal transplantation is the only solution at present. However, corneal transplantation requires a fresh human cornea and there is a worldwide shortage of donors. Therefore, finding new functional CECs to replace human CECs is urgent. Skin-derived precursors (SKPs) can be easily acquired and have multiple differential potential. We co-cultured human SKPs with B4G12 cells in serum-free medium and obtained abundant CEC-like cells which had similar morphology and characteristic to human CECs. CEC-like cells exerted excellent therapeutic effect when they were transplanted into rabbit and monkey corneal endothelial dysfunction models by injection method. This protocol enables efficient production of CEC-like cells from SKPs. The renewable cell source, novel derivation method and simple treatment strategy may lead to potential applications in cell replacement therapy for corneal endothelial dysfunction.Corneal endothelial cells (CECs) are a monolayer of hexagonal cells covering the posterior surface of the cornea, and serve as a barrier between the corneal stroma and the aqueous humor. The tight junction as well as the ionic "pump" functions of CECs are important factors that maintain corneal transparency 1 . Many pathological factors such as trauma, surgery, and inflammation may cause dramatic loss of endothelial cell density, resulting in corneal endothelial dysfunction which is characterized by corneal edema, bullous keratopathy, and loss of visual acuity 2 . Human CECs have limited proliferative capacity in vivo and cannot be subcultured for more than a few passages in vitro 3,4 . Corneal transplantation is the only solution at present. However, corneal transplantation requires a fresh human cornea and there is a worldwide shortage of donors 5 . Therefore, finding new functional CECs to replace human CECs is a new and potential direction for future clinical application.Skin-derived precursors (SKPs) were discovered by Tomas 6,7 , and they have been a research hotspot in recent years. SKPs can be isolated in large quantities from many parts of the skin in rodents and humans. As they are multipotent, they can be differentiated into several functional cell types 8 , and are able to suppress the allogeneic activation of T-lymphocytes resulting in an improved health status of animals suffering from a graft-versus-host reaction 9 . Moreover, SKPs were demonstrated to be embryonic neural-crest-related precursors and share many properties with neural crest stem cells 10

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“…Progenitors derived from corneal stroma can be differentiated into corneal endothelial cells. But, the access to these corneal progenitors is limited by a shortage of donors and by their limited proliferative capacity . SKPs isolated from the facial skin of mice can represent an accessible source of neural‐crest‐derived cells, which are able to differentiate into corneal endothelial‐like cells when cultured with retinoic acid and GSK 3‐β inhibitor.…”

Section: Potential Use Of Skps In Regenerative Medicinementioning

confidence: 99%

Multipotentiality of skin‐derived precursors: application to the regeneration of skin and other tissues

Bergeron

Busuttil

Botto

2020

Intern J of Cosmetic Sci

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Skin-derived precursors (SKPs) have been described as multipotent dermal precursors. Here, we provide a review of the breadth and depth of scientific literature and studies regarding SKPs, accounting for a large number of scientific publications. Interestingly, these progenitors can be isolated from embryonic and adult skin, as well as from a population of dermal cells cultured in vitro in monolayer. Gathering information from different authors, this review explores different aspects of the SKP theme, such as the potential distinct origins of SKPs in rodents and in humans, and also their ability to differentiate in vitro and in vivo into multiple lineages of different progeny. This remarkable capacity makes SKPs an interesting endogenous source of precursors to explore in the framework of experimental and therapeutic applications in different domains. SKPs are not only involved in the skin's dermal maintenance and support as well as wound healing, but also in hair follicle morphogenesis. This review points out the interests of future researches on SKPs for innovative perspectives that may be helpful in many different types of scientific and medical domains. exp erimentations en cours et a venir concernant les SKPs en vue de perspectives innovantes pouvant influencer la recherche dans de nombreux domaines scientifiques et m edicaux.

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Skin-Derived Precursors as a Source of Progenitors for Corneal Endothelial Regeneration

Cited by 41 publications

References 39 publications

Variable Responses to Corneal Grafts: Insights from Immunology and Systems Biology

Variable Responses to Corneal Grafts: Insights from Immunology and Systems Biology

Therapy of corneal endothelial dysfunction with corneal endothelial cell-like cells derived from skin-derived precursors

Multipotentiality of skin‐derived precursors: application to the regeneration of skin and other tissues

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