Skin Diseases Modeling using Combined Tissue Engineering and Microfluidic Technologies

Mohammadi, Mohammad Hossein; Araghi, Behnaz Heidary; Beydaghi, Vahid; Geraili, Armin; Moradi, Farshid; Jafari, Parya; Janmaleki, Mohsen; Valente, Karolina Papera; Akbari, Mohsen; Sanati‐Nezhad, Amir

doi:10.1002/adhm.201600439

Cited by 63 publications

(52 citation statements)

References 158 publications

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“…With the advancement of novel biomaterials and microfluidic systems [190][191][192][193], new approaches, such as 3D bioprinting technology, can potentially be applied to fabricate skin-on-a-chip systems with an ECM embedded and spatial heterogeneity incorporated. This may further facilitate the development of more representative microfluidic skin disease models in the future [194].…”

Section: Skin-on-a-chipmentioning

confidence: 99%

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

Shi

Wang

et al. 2019

Biomaterials

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A B S T R A C TBacterial infections and antibiotic resistant bacteria have become a growing problem over the past decade. As a result, the Centers for Disease Control predict more deaths resulting from microorganisms than all cancers combined by 2050. Currently, many traditional models used to study bacterial infections fail to precisely replicate the in vivo bacterial environment. These models often fail to incorporate fluid flow, bio-mechanical cues, intercellular interactions, host-bacteria interactions, and even the simple inclusion of relevant physiological proteins in culture media. As a result of these inadequate models, there is often a poor correlation between in vitro and in vivo assays, limiting therapeutic potential. Thus, the urgency to establish in vitro and ex vivo systems to investigate the mechanisms underlying bacterial infections and to discover new-age therapeutics against bacterial infections is dire. In this review, we present an update of current in vitro and ex vivo models that are comprehensively changing the landscape of traditional microbiology assays. Further, we provide a comparative analysis of previous research on various established organ-disease models. Lastly, we provide insight on future techniques that may more accurately test new formulations to meet the growing demand of antibiotic resistant bacterial infections. Lack of adequate model systemsCommonly, a variety of inconsistent, in vitro and in vivo models have been progressively utilized for antibiotic/drug development and pathophysiological studies. These systems range from simple in vitro models using microtiter plate assays and flow cells to more complex in https://doi.

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Section: Skin-on-a-chipmentioning

confidence: 99%

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

Shi

Wang

et al. 2019

Biomaterials

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“…An organ‐on‐chip consists of a microfluidic device composed of a variety of cell types cultured in different layers that can interact with each other, in a highly controlled microenvironment, while mimicking the complex cell–cell and cell–matrix interactions. These platforms provide spatiotemporal chemical gradients and dynamic biomechanical environments for living organs, allowing the development of biomimetic tissues and organs as stand‐alone models for drug discovery applications . Such platforms often utilize 2D microchannels, however, an ideal wound model enables the assembly of 3D cultures.…”

Section: Introductionmentioning

confidence: 99%

Simulating Inflammation in a Wound Microenvironment Using a Dermal Wound‐on‐a‐Chip Model

Biglari

Tan

et al. 2018

Adv Healthcare Materials

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Considerable progress has been made in the field of microfluidics to develop complex systems for modeling human skin and dermal wound healing processes. While microfluidic models have attempted to integrate multiple cell types and/or 3D culture systems, to date they have lacked some elements needed to fully represent dermal wound healing. This paper describes a cost‐effective, multicellular microfluidic system that mimics the paracrine component of early inflammation close to normal wound healing. Collagen and Matrigel are tested as materials for coating and adhesion of dermal fibroblasts and human umbilical vein endothelial cells (HUVECs). The wound‐on‐chip model consists of three interconnecting channels and is able to simulate wound inflammation by adding tumor necrosis factor alpha (TNF‐α) or by triculturing with macrophages. Both the approaches significantly increase IL‐1β, IL‐6, IL‐8 in the supernatant (p < 0.05), and increases in cytokine levels are attenuated by cotreatment with an anti‐inflammatory agent, Dexamethasone. Incorporation of M1 and M2 macrophages cocultured with fibroblasts and HUVECs leads to a stimulation of cytokine production as well as vascular structure formation, particularly with M2 macrophages. In summary, this wound‐on‐chip system can be used to model the paracrine component of the early inflammatory phase of wound healing and has the potential for the screening of anti‐inflammatory compounds.

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“…The organ-on-chip technology may also allow integration of sensors for real-time readout of biomarkers. Additionally, in combination with iPSCs it is envisioned that different organ-models can be created and combined to create a patient specific multi-organ-on-chip model as a highly advanced tool for drug development [4–6]. Here we discuss the state of the art with regards to skin-on-chip models and cell sources (primary, cell line, induced pluripotent stem cells) that may enable the next step to be taken in skin disease modeling, substance testing, and ultimately personalized medicine.…”

Section: Introductionmentioning

confidence: 99%

Progress and Future Prospectives in Skin-on-Chip Development with Emphasis on the use of Different Cell Types and Technical Challenges

Broek

Bergers

Reijnders

et al. 2017

Stem Cell Rev and Rep

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Understanding the healthy and diseased state of skin is important in many areas of basic and applied research. Although the field of skin tissue engineering has advanced greatly over the last years, current in vitro skin models still do not mimic the complexity of the human skin. Skin-on-chip and induced pluripotent stem cells (iPSC) might be key technologies to improve in vitro skin models. This review summarizes the state of the art of in vitro skin models with regard to cell sources (primary, cell line, iPSC) and microfluidic devices. It can be concluded that iPSC have the potential to be differentiated into many kinds of immunologically matched cells and skin-on-chip technology might lead to more physiologically relevant skin models due to the controlled environment, possible exchange of immune cells, and an increased barrier function. Therefore the combination of iPSC and skin-on-chip is expected to lead to superior healthy and diseased in vitro skin models.

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Skin Diseases Modeling using Combined Tissue Engineering and Microfluidic Technologies

Cited by 63 publications

References 158 publications

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

Simulating Inflammation in a Wound Microenvironment Using a Dermal Wound‐on‐a‐Chip Model

Progress and Future Prospectives in Skin-on-Chip Development with Emphasis on the use of Different Cell Types and Technical Challenges

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