Skin epithelial cells in mice from umbilical cord blood mesenchymal stem cells

Dai, Yucheng; Li, Jian; Li, Jie; Dai, Ge; Mu, Haiyan; Wu, Qiong; Hu, Kuikui; Cao, Qing

doi:10.1016/j.burns.2006.08.028

Cited by 42 publications

(29 citation statements)

References 30 publications

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“…(Yoshikawa et al 2008;Sasaki et al 2008) MSCs not only participate in the regeneration of tissue of mesenchymal lineage such as intervertebral disc cartilage (Crevensten et al 2004), bone (Arinzeh et al 2003), and cardiomyocytes (Fukuda 2002), but also appear to differentiate into cells derived from other embryonic layers, including skin (Sasaki et al 2008). In the most pervious studies, MSCs were isolated from bone marrow and other tissue such as adipose tissue, nerve tissue, umbilical cord blood and dermis (Kim et al 2007;Dai et al 2007;Perng et al 2006;Shih et al 2005;Jones et al 2002;Luo et al 2010;Tark et al 2010). These experiments demonstrated the plasticity of MSCs and their potential usefulness in complex tissue repair and regeneration and in cell therapy (Fu and Li 2009).…”

Section: Discussionmentioning

confidence: 97%

Effects of transplanted mesenchymal stem cells isolated from Wharton’s jelly of caprine umbilical cord on cutaneous wound healing; histopathological evaluation

et al. 2011

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The aim of this study was to investigate the effects of transplanted Wharton's jelly mesenchymal stem cells (WJMSCs) of caprine umbilical cord on cutaneous wound healing process in goat. After collection of caprine pregnant uterus of mixed breed goats from abattoir, the Wharton's jelly (WJ) of umbilical cord was harvested. The tissues were minced in ventilated flasks and explant culture method was used for separating mesenchymal stem cells (MSCs). The isolated cells were immunostained for Actin protein, histochemically assayed for the presence of alkaline phosphatase activity, and analyzed for detection of matrix receptors (CD44) and hematopoetic lineage markers (CD34), using flow cytometery. After The isolated cells, 3×10(6) MSCs were stained with BrdU and prepared for transplantation to each wound. Four 3-cm linear full thickness skin incisions were made on both sides of thoracic vertebrate of four Raeini goats (two wounds on each side). The left wounds were implanted with MSCs in 0.6 ml of Phosphate buffer saline (PBS), and the right wounds considered as control group that received 0.6 ml of PBS. The samples were taken from the wounds 7 and 12 days after the wounding, and healing process was compared histologically between the two groups. Anti-BrdU staining showed that the transplanted cells were still alive in the wound bed during the study. The histopathological study revealed that re-epithelialization was complete at days 7 in treated wounds with WJMSCs, whereas in control wound the wounds still showed incomplete epithelialization 12 days after wounding. Also, microscopic evaluation showed less inflammation, thinner granulation tissue formation with minimum scar in the treated wounds in comparison with control wounds. In conclusion, this study demonstrates the beneficial effect of caprine WJMSCs in cutaneous wound healing in goat.

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Section: Discussionmentioning

confidence: 97%

Effects of transplanted mesenchymal stem cells isolated from Wharton’s jelly of caprine umbilical cord on cutaneous wound healing; histopathological evaluation

et al. 2011

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“…Of note, the regeneration of injured or damaged epitheliumlined organs requires the coordination of multiple types of cells, the epithelial, endothelial, mesenchymal, and immune cells [38]. Recent studies have shown that mesenchymal stem cells (MSCs) participate in the regeneration and repair of a variety of diseased epithelial tissues, including injured epithelial layers in skin [39], airway [40], cornea [41], gastric and intestine [42,43], kidney [44], and oral cavity [45]. The mechanisms underlying MSC-mediated regeneration of injured epithelial tissues may involve not only the secretion of various factors with antioxidant, anti-inflammatory, antiapoptotic, or proangiogenic functions, but also the transdifferentiation of MSCs into epithelial-like cells possibly through MET [44].…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Spheroid Culture of Human Gingiva-Derived Mesenchymal Stem Cells Enhances Mitigation of Chemotherapy-Induced Oral Mucositis

Zhang

Nguyen

Shi

et al. 2012

Stem Cells and Development

157

150

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Mesenchymal stem cells (MSCs) are capable of regenerative and immunomodulatory functions in cell-based therapies in a variety of human diseases and injuries; however, their therapeutic efficacy and potential side effects remain major obstacles in clinical applications. We report here a 3D spheroid culture approach to optimize stem cell properties and therapeutic effects of human gingiva-derived mesenchymal stem cells (GMSCs) in mitigation of experimental oral mucositis. Under growth condition of ultra-low attachment, GMSCs spontaneously aggregated into 3D spheroids and exhibited distinct early stem cell phenotype characterized by elevated expression Stro-1 and CXC chemokine receptor 4 (CXCR-4) as well as OCT-4 and Nanog, 2 important transcriptional factors relevant to stem cell properties, and decreased expression of MSC-associated markers, including CD29, CD90, and CD105. Functionally, spheroid GMSCs are capable of enhanced multipotency and augmented secretion of several chemokines and cytokines relevant to cell migration, survival, and angiogenesis. More importantly, spheroid GMSCs expressed increased levels of reactive oxygen species, hypoxia-inducible factor (HIF)-1 and -2a, and manganese superoxide dismutase, which correlated with improved resistance to oxidative stress-induced apoptosis. Using an in vivo murine model of chemotherapy-induced oral mucositis, we demonstrated that spheroid-derived GMSCs possessed better therapeutic efficacy than their adherent cells in reversing body weight loss and promoting the regeneration of disrupted epithelial lining of the mucositic tongues. These findings suggest that 3D spheroid culture allows early stemness preservation and potentially precondition GMSCs for enhanced mitigation of oral mucositis.

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“…The use of adult stem cells, especially mesenchymal stem cells (MSCs), is not burdened by many of these problems. MSCs can be isolated from bone marrow and other tissue such as adipose tissue, nerve tissue, umbilical cord blood, and dermis (Kim et al 2007;Dai et al 2007;Perng et al 2006;Shih et al 2005;Jones et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cells and skin wound repair and regeneration: possibilities and questions

2008

Cell Tissue Res

122

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Adult mesenchymal stem cells (MSCs) have the capacity for self-renewal and for differentiating into a variety of cells and tissues. They may leave their niche to migrate to remote tissues and play a critical role in wound repair and tissue regeneration. Because of their multipotency, easy isolation and culture, highly expansive potential, and immunosuppression properties, these cells may be an attractive therapeutic tool for regenerative medicine and tissue engineering. Several studies have indicated a contribution of MSCs to reconstituting skin in cutaneous wounds, but problems still need resolution before MSCs can be widely used clinically. This review focuses mainly on the benefits of MSCs in skin wound healing and tissue regeneration and on the questions that remain to be answered before MSCs can be used in clinical practice.

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Skin epithelial cells in mice from umbilical cord blood mesenchymal stem cells

Cited by 42 publications

References 30 publications

Effects of transplanted mesenchymal stem cells isolated from Wharton’s jelly of caprine umbilical cord on cutaneous wound healing; histopathological evaluation

Effects of transplanted mesenchymal stem cells isolated from Wharton’s jelly of caprine umbilical cord on cutaneous wound healing; histopathological evaluation

Three-Dimensional Spheroid Culture of Human Gingiva-Derived Mesenchymal Stem Cells Enhances Mitigation of Chemotherapy-Induced Oral Mucositis

Mesenchymal stem cells and skin wound repair and regeneration: possibilities and questions

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