Skin-infiltrating neutrophils following exposure to solar-simulated radiation could play an important role in photoageing of human skin

Rijken, Feiko; Kiekens, Rebecca C. M.; Bruijnzeel, P. L. B.

doi:10.1111/j.1365-2133.2004.06335.x

Cited by 77 publications

(81 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is known that UV-induced MMPs are secreted by diverse cells, including infiltrated inflammatory cells. 21,22 We found that the inflammatory infiltrates were significantly decreased after the PDT. Therefore, the net effects of the induction of TFG-b and reduction of the degrading enzymes from the inflammatory cells likely contributed to the decreased expression of MMPs after the ALA-PDT.…”

Section: Discussionmentioning

confidence: 79%

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Park

Sohn

Lee

et al. 2010

Journal of the American Academy of Dermatology

View full text Add to dashboard Cite

Background: Repeated exposure to ultraviolet radiation from the sun results in premature photoaging. Photodynamic therapy (PDT) has been shown to be effective for treatment of photoaging, although the data from most studies have been based on clinical observation.Objectives: We investigated whether 5-aminolevulinic acid (ALA)-PDT induced histologic changes suggesting photorejuvenation.Methods: Fourteen patients with one to three actinic keratoses on the face were treated twice with ALA-PDT by using a 1200 W metal halogen lamp at 1-month intervals. Skin biopsy before and 1 month after the PDT was performed. Twenty-five pairs of specimens were obtained. We examined the specimens with routine and immunohistochemical staining and evaluated the parameters associated with photoaging by using image analysis.Results: After ALA-PDT, the mean epidermal thickness and dermal inflammatory infiltrate were reduced. The total collagen volume in the dermis significantly increased with expression of type I and III procollagen. The level of transforming growth factor b and transforming growth factor b type II receptors in the epidermis also increased. The elastotic material with co-localizing fibrillin-1 and tropoelastin expression in the dermis decreased after treatment. The expression of matrix metalloproteinases-1, -3, and -12 also decreased.Limitations: The study was limited by the small sample size.Conclusions: ALA-PDT resulted in histological changes indicating restoration of photoaged skin. These data suggest that ALA-PDT could be effective for photorejuvenation. ( J Am Acad Dermatol 2010;62:85-95.)

show abstract

Section: Discussionmentioning

confidence: 79%

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Park

Sohn

Lee

et al. 2010

Journal of the American Academy of Dermatology

View full text Add to dashboard Cite

show abstract

“…The structural integrity of the ECM is compromised with the increase in the basal or induced levels of MMPs/elastase enzymes as well as their activity with aging or exposure to UV radiation. The ECM proteolytic enzymes (MMPs/elastases) are produced by epidermal keratinocytes, dermal fibroblasts, neutrophils and melanoma cells in the mediation of skin aging or cancer [3,[15][16][17][18]. P. leucotomos extract directly inhibits the activities of MMPs (MMP-1, 2, 3, 9) as well as the cellular expression of MMPs (MMP-1, 2) in nonirradiated or UV radiated dermal fibroblasts or epidermal keratinocytes [2,3].…”

Section: Discussionmentioning

confidence: 99%

Beneficial Regulation of Elastase Activity and Expression of Tissue Inhibitors of Matrixmetalloproteinases, Fibrillin, Transforming Growth Factor-β, and Heat Shock Proteins by P. leucotomos in Nonirradiated or Ultraviolet-Radiated Epidermal Keratinocytes

Philips

González

2013

ISRN Oxidative Medicine

View full text Add to dashboard Cite

There is loss of the structural integrity of the extracellular matrix (ECM) with intrinsic aging as well as photoaging, largely due to reactive oxygen species (ROS). The structural ECM proteins include the microfibrils that are composed of fibrillin. The structural ECM proteins are primarily degraded by the matrixmetalloproteinases (MMPs) and elastase enzymes. The MMPs are inhibited by the tissue inhibitors of MMPs (TIMPs). A primary regulator of the ECM proteins is transforming growth factor-(TGF-), and the chaperone proteins important for its formation are the heat shock proteins (HSP). P. leucotomos extract beneficially regulates of MMPs, TIMPs, and TGF-in nonirradiated or ultraviolet (UV) radiated fibroblasts and melanoma cells. The hypothesis of this research was that the antioxidant activity or chemistry of P. leucotomos extract would also directly inhibit elastase activity, stimulate the cellular expression of TIMPs, fibrillins, and TGF-, and regulate HSPs in nonirradiated and UVA or UVB radiated epidermal keratinocytes. P. leucotomos directly inhibited elastase activity, stimulated the cellular expression of TIMPs, fibrillins, and TGF-, and differentially regulated HSPs in nonirradiated and UVA or UVB radiated epidermal keratinocytes. We infer that the P. leucotomos extract strengthens the ECM and is effective in the prevention or treatment of intrinsic and photoaging of skin.

show abstract

“…Exposure of skin or skin-derived cells to UVR, for example, is known to upregulate the expression of many elastic fibre degrading enzymes, including MMP-2, -3, -9, -12 and -13 and the serine protease neutrophil elastase (Fisher et al 1996;Kim et al 2006;Rijken et al 2005;Saarialho-Kere et al 1999). ROS generation may mediate elastic fibre damage in skin either indirectly, via protease upregulation (Yaar and Gilchrest 2007), or directly via interaction with the sulphur containing amino acids methionine and cysteine (Haenold et al 2005).…”

Section: Cutaneousmentioning

confidence: 99%

Tissue elasticity and the ageing elastic fibre

Sherratt

2009

AGE

270

189

View full text Add to dashboard Cite

The ability of elastic tissues to deform under physiological forces and to subsequently release stored energy to drive passive recoil is vital to the function of many dynamic tissues. Within vertebrates, elastic fibres allow arteries and lungs to expand and contract, thus controlling variations in blood pressure and returning the pulmonary system to a resting state. Elastic fibres are composite structures composed of a cross-linked elastin core and an outer layer of fibrillin microfibrils. These two components perform distinct roles; elastin stores energy and drives passive recoil, whilst fibrillin microfibrils direct elastogenesis, mediate cell signalling, maintain tissue homeostasis via TGFβ sequestration and potentially act to reinforce the elastic fibre. In many tissues reduced elasticity, as a result of compromised elastic fibre function, becomes increasingly prevalent with age and contributes significantly to the burden of human morbidity and mortality. This review considers how the unique molecular structure, tissue distribution and longevity of elastic fibres pre-disposes these abundant extracellular matrix structures to the accumulation of damage in ageing dermal, pulmonary and vascular tissues. As compromised elasticity is a common feature of ageing dynamic tissues, the development of strategies to prevent, limit or reverse this loss of function will play a key role in reducing age-related morbidity and mortality.

show abstract

Skin-infiltrating neutrophils following exposure to solar-simulated radiation could play an important role in photoageing of human skin

Abstract: Our study suggests that neutrophils participate in the process of photoageing of human skin as they infiltrate the skin and release enzymatically active elastase (neutrophil elastase), collagenase (MMP-1) and gelatinase (MMP-9).

Cited by 77 publications

References 14 publications

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Beneficial Regulation of Elastase Activity and Expression of Tissue Inhibitors of Matrixmetalloproteinases, Fibrillin, Transforming Growth Factor-β, and Heat Shock Proteins by P. leucotomos in Nonirradiated or Ultraviolet-Radiated Epidermal Keratinocytes

Tissue elasticity and the ageing elastic fibre

Contact Info

Product

Resources

About