Skin-Lightening Effect of a Polyphenol Extract from Acerola (<i>Malpighia emarginata</i>DC.) Fruit on UV-Induced Pigmentation

Hanamura, Takayuki; Uchida, Eriko; Aoki, Hitoshi

doi:10.1271/bbb.80421

Cited by 58 publications

(39 citation statements)

References 35 publications

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“…Caffeic acid, ferulic acid, myricetin and quercetin were detected in leaves of H. moellendorffii. Interestingly, these polyphenols have been partially reported to have both antioxidative and anti-melanogenic effects in acerola [28]. As for the antimelanogenic effects of antioxidants, quercetin was shown to inhibit melanin biosynthesis in B16 cells via a decrease in the protein level of tyrosinase [29].…”

Section: Discussionmentioning

confidence: 99%

Anti-Melanogenic Activities of Heracleum moellendorffii via ERK1/2-Mediated MITF Downregulation

Alam

Seo

Zhao

et al. 2016

IJMS

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In this study, the anti-melanogenic effects of Heracleum moellendorffii Hance extract (HmHe) and the mechanisms through which it inhibits melanogenesis in melan-a cells were investigated. Mushroom tyrosinase (TYR) activity and melanin content as well as cellular tyrosinase activity were measured in the cells. mRNA and protein expression of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), TYR-related protein-1 (TYRP-1) and -2 were also examined. The results demonstrate that treatment with HmHe significantly inhibits mushroom tyrosinase activity. Furthermore, HmHe also markedly inhibits melanin production and intracellular tyrosinase activity. By suppressing the expression of TYR, TYRP-1, TYRP-2, and MITF, HmHe treatment antagonized melanin production in melan-a cells. Additionally, HmHe interfered with the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, with reversal of HmHe-induced melanogenesis inhibition after treatment with specific inhibitor U0126. In summary, HmHe can be said to stimulate ERK1/2 phosphorylation and subsequent degradation of MITF, resulting in suppression of melanogenic enzymes and melanin production, possibly due to the presence of polyphenolic compounds.

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Section: Discussionmentioning

confidence: 99%

Anti-Melanogenic Activities of Heracleum moellendorffii via ERK1/2-Mediated MITF Downregulation

Alam

Seo

Zhao

et al. 2016

IJMS

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“…We previously investigated variations in the polyphenol composition of acerola and found that cyanidin-3-α-O-rhamnoside, pelargonidin-3-α-O-rhamnoside, quercetin-3-α-O-rhamnoside, kaempferol glycosides, astilbin and proanthocyanidin are present [14, 15, 17]. Additionally, we revealed that polyphenol extracts from acerola and individual polyphenols (cyanidin-3-α-O-rhamnoside, pelargonidin-3-α-O-rhamnoside and Astilbin) inhibit tyrosinase activity [23]. Previous studies have indicated that polyphenol extracts inhibit melanogenesis through the down-regulations of Tyr , Tyrp1 , and Dct [33–35].…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, we have demonstrated a beneficial effect of acerola in humans; i.e., the intake of acerola juice (AJ) affects the absorption of AA in plasma and minimizes its excretion via urine in healthy Japanese subjects, which suggests improvement of the bioavailability of AA in humans [22]. In the skin, we have previously reported that the intake of acerola polyphenol extracts prevents UVB-induced skin pigmentation in brownish guinea pigs, which possess moderate numbers of melanocytes and melanosomes in the epidermis [23]. Because acerola contains not only a large amount of AA but also beneficial ingredients as noted above, it is important to elucidate the effect of whole juice intake in UVB-irradiated skin.…”

Section: Introductionmentioning

confidence: 99%

Acerola (Malpighia emarginata DC.) Juice Intake Suppresses UVB-Induced Skin Pigmentation in SMP30/GNL Knockout Hairless Mice

Sato

Uchida

Aoki³

et al. 2017

PLoS ONE

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Background/AimsAcerola (Malpighia emarginata DC.) is a fruit that is known to contain high amounts of ascorbic acid (AA) and various phytochemicals. We have previously reported that AA deficiency leads to ultraviolet B (UVB)-induced skin pigmentation in senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) hairless mice. The present study was undertaken to investigate the effects of acerola juice (AJ) intake on the skin of UVB-irradiated SMP30/GNL KO mice.Research design/Principal findingsFive-week old hairless mice were given drinking water containing physiologically sufficient AA (1.5 g/L) [AA (+)], no AA [AA (-)] or 1.67% acerola juice [AJ]. All mice were exposed to UVB irradiation for 6 weeks. UVB irradiation was performed three times per week. The dorsal skin color and stratum corneum water content were measured every weekly, and finally, the AA contents of the skin was determined. The skin AA and stratum corneum water content was similar between the AA (+) and AJ groups. The L* value of the AA (+) group was significantly decreased by UVB irradiation, whereas AJ intake suppressed the decrease in the L* value throughout the experiment. Moreover, in the AJ group, there was a significant decrease in the expression level of dopachrome tautomerase, an enzyme that is involved in melanin biosynthesis.ConclusionThese results indicate that AJ intake is effective in suppressing UVB-induced skin pigmentation by inhibiting melanogenesis-related genes.

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“…They were housed in wire mesh cages under standard experimental conditions and were fed on standard diet with free access to water. After one week of acclimatization to the new environment, the animals were subjected to gentle shaving of the hair over the center of their backs with an electric clipper (HC7235, United, Greece) at a surface area measuring 2 cm x 2 cm after sterilization of the area with betadine [19] . Then, random picking of animals from their cages was done to classify them into two groups, ten animals each.…”

Section: Methodsmentioning

confidence: 99%

Effect of Adipose-Derived Stem Cells on Induced Photoaging in the Skin of Adult Guinea Pig: Histological and Immunohistochemical Study

Mohammed

Salam

Kalleny

et al. 2017

Egyptian Journal of Histology

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Introduction:The term photoaging describes the sun damaging effects on skin mainly due to chronic ultraviolet (UV) light exposure. The unsatisfactory results of the available anti-aging strategies raise the demand for alternative forms of treatments. Adipose-derived stem cells (ASCs) are available in abundant quantities, harvested by a minimally invasive procedure, safely transplanted and differentiated along multiple cell lineages. Subsequently, used in treatment of many diseases. Aim: To assess the potential ability of ASCs to ameliorate skin changes induced by chronic exposure to artificial light source similar to the sun rays in its UVA and UVB spectrum in adult female guinea pigs. Material and Methods: Adipose-derived stem cells were isolated from subcutaneous white adipose tissue of five adult human donors undergoing elective liposuction surgery. Twenty adult female guinea pigs were used and were randomly divided into two groups each was subdivided into two subgroups "five animals, each". Subgroup IA served as the control group. Subgroup IB was intradermally injected with phosphate buffered saline solution. Subgroup IIA served as the photoaging model. Subgroup IIB served as the photoaging model intradermaly injected with ASCs. Isolated stem cells were cultured and characterized. Skin specimens were prepared and examined using different histological and immunohistochemical techniques. Morphometric and statistical studies were also performed. Results: Subgroup IIA showed various UV damaging effects in the skin epidermis and dermis, while ASCs injection in subgroup IIB resulted in partial restoration of the skin structure. Conclusion: Intradermal injection of ASCs partially ameliorated the photo-damaging effects. Further studies are needed before ASCs clinical application.

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Skin-Lightening Effect of a Polyphenol Extract from Acerola (Malpighia emarginataDC.) Fruit on UV-Induced Pigmentation

Cited by 58 publications

References 35 publications

Anti-Melanogenic Activities of Heracleum moellendorffii via ERK1/2-Mediated MITF Downregulation

Anti-Melanogenic Activities of Heracleum moellendorffii via ERK1/2-Mediated MITF Downregulation

Acerola (Malpighia emarginata DC.) Juice Intake Suppresses UVB-Induced Skin Pigmentation in SMP30/GNL Knockout Hairless Mice

Effect of Adipose-Derived Stem Cells on Induced Photoaging in the Skin of Adult Guinea Pig: Histological and Immunohistochemical Study

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