Skin penetration behaviour of liposomes as a function of their composition

Gillet, Aline; Lecomte, Frédéric; Hubert, Pascale; Ducat, Emilie; Évrard, Brigitte; Piel, Géraldine

doi:10.1016/j.ejpb.2011.01.011

Cited by 101 publications

(62 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It can be incorporated into the lipid bilayers promoting a marking and also mimicking lipophilic drugs such as betamethasone, since they both exhibit a similar partition coefficient (log P). In many cases, it is associated with a hydrophilic dye as, for example, calcein, which is expected to be encapsulated in the aqueous compartment of the liposome, promoting marking and functioning as a model for hydrophilic drugs that can be carried by this type of delivery system [26,37].…”

Section: Main Fluorescence Probes Used To Assess Skin Penetrationmentioning

confidence: 99%

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

Rossetti¹,

Depieri²,

Bentley³

2013

Confocal Laser Microscopy - Principles and Applications in Medicine, Biology, and the Food Sciences

View full text Add to dashboard Cite

Section: Main Fluorescence Probes Used To Assess Skin Penetrationmentioning

confidence: 99%

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

Rossetti¹,

Depieri²,

Bentley³

2013

Confocal Laser Microscopy - Principles and Applications in Medicine, Biology, and the Food Sciences

View full text Add to dashboard Cite

“…Calcein penetrated to a lower extent, as expected, since it is a hydrophilic marker with low logP (-5.02) (Gillet et al, 2011) with limited skin penetration potential (Bolzinger et al, 2012). Interestingly, calcein was detected in the perfusate, which would suggest that the circulation enabled its penetration.…”

Section: Discussionmentioning

confidence: 80%

The isolated perfused human skin flap model: A missing link in skin penetration studies?

Ternullo

Weerd

Flaten

et al. 2017

European Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Development of effective (trans)dermal drug delivery systems requires reliable skin models to evaluate skin drug penetration. The isolated perfused human skin flap remains metabolically active tissue for up to 6 hours during in vitro perfusion. We introduce the isolated perfused human skin flap as a close-to-in vivo skin penetration model. To validate the model's ability to evaluate skin drug penetration the solutions of a hydrophilic (calcein) and a lipophilic (rhodamine) fluorescence marker were applied. The skin flaps were perfused with modified Krebs-Henseleit buffer (pH 7.4).Infrared technology was used to monitor perfusion and to select a well-perfused skin area for administration of the markers. Flap perfusion and physiological parameters were maintained constant during the 6 hours experiments and the amount of markers in the perfusate was determined. Calcein was detected in the perfusate, whereas rhodamine was not detectable. Confocal images of skin cross-sections shoved that calcein was uniformly distributed through the skin, whereas rhodamine accumulated in the stratum corneum. For comparison, the penetration of both markers was evaluated on ex vivo human skin, pig skin and cellophane membrane. The proposed perfused flap model enabled us to distinguish between the penetrations of the two markers and could be a promising close-to-in vivo tool in skin penetration studies and optimization of formulations destined for skin administration.

show abstract

“…Betamethasone is released from the vesicles, after free drug molecules diffuse through the stratum corneum and partition into the viable skin tissue ( Figure 2). It was observed that the encapsulation into the lipid bilayer significantly enhanced the accumulation of betamethasone in the epidermis of pig ear skin [65]. Negatively charged liposomes significantly enhanced betamethasone penetration in the epidermidis compared to positively charged or neutral liposomes [66].…”

Section: Liposomal Carriersmentioning

confidence: 89%

Innovative Formulations for the Controlled and Site-specific Delivery of Antiinflammatory Drugs

Serpe¹,

Canaparo²,

Foglietta³

et al. 2013

CPD

View full text Add to dashboard Cite

Pharmaceutical technology has introduced a promising pathway in the future of medicinein particular nanotechnological innovations have provided the opportunity to design and develop efficient drug delivery systems able to target and treat several diseases, including those mediated by inflammation. The engineering of drug delivery systems can be used to target tissues involved in the pathology under treatment, to avoid early drug biological environmental degradation and to modulate drug pharmacokinetics. Glucocorticoids and non-steroidal anti-inflammatory drugs are the most commonly prescribed drug categories worldwide for the treatment of disorders associated with inflammation.Although glucocorticoids can be highly effective in treating inflammation, their systemic application is limited due to the high incidence of serious adverse effects, mainly in long-term treatment. Non-steroidal anti-inflammatory drugs are a heterogeneous group of compounds and most of them have unfavorable pharmacokinetics and pharmacodynamics, leading to adverse effects, such as gastrointestinal disorders. Therefore, the need for drug delivery systems for long term administration of anti-inflammatory drugs witha well-controlled release profile is evident. The aim of this review is to assess innovative colloidal drugs carriers, in particular liposomes and nanoparticles, with special focus on site-specific delivery for particularly problematic tissues such as the gastrointestinal tract, joints and eyes.3

show abstract

Skin penetration behaviour of liposomes as a function of their composition

Cited by 101 publications

References 28 publications

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

The isolated perfused human skin flap model: A missing link in skin penetration studies?

Innovative Formulations for the Controlled and Site-specific Delivery of Antiinflammatory Drugs

Contact Info

Product

Resources

About