Skin Prolylhydroxylase Activity and Wound Healing

Abstract

The relationship between wound healing and skin prolylhydroxylase activity was examined in 212 patients undergoing reconstructive procedures and 109 controls using skin prolylhydroxylase activity as an index of collagen synthesis. In the control group, the median value of skin prolylhydroxylase activity was 360 cpm/mg protein. There was a higher skin prolylhydroxylase activity in 274 patients with successful healing (368 cpm/mg protein) than there was in 33 patients who required local wound care (202 cpm/mg protein) and 14 patients with severe local complications (71 cpm/mg protein, p = 0.02). Skin prolylhydroxylase activity was also significantly lower (p = 0.002) in patients with laboratory criteria for malnutrition (albumin <3.5 g/dl, 108 cpm/mg protein; total lymphocyte count <1,500 mm³, 211 cpm/mg protein, and albumin <3.5 g/dl + lymphocyte count < 1,500 mm³, 52 cpm/mg protein), but age, diabetes mellitus, malignancy and wound infection did not affect skin prolylhydroxylase activity. These results suggest that skin prolylhydroxylase activity is reduced in patients with malnutrition and delayed wound healing.

Collagen of Chronically Inflamed Skin Is Over-Modified and Upregulates Secretion of Matrix Metalloproteinase 2 and Matrix-Degrading Enzymes by Endothelial Cells and Fibroblasts

“…These results indicate that a2(I) in DNFB-treated skin has a di¡erent susceptibility to V8 protease. In£ammation and wound healing activate 4 -prolylhydroxylase, which hydroxylates proline (Fullana et al, 1993). Therefore, the degree of hydroxylation of proline and lysine residues was examined.…”

“…In£ammation and wound healing activate 4 -prolylhydroxylase, which hydroxylates proline (Fullana et al, 1993). Therefore, it is conceivable that the activity of 4 -prolylhydroxylase is increased .…”

Effects of streptozotocin‐induced diabetes, physical training and their combination on collagen biosynthesis in rat skeletal muscle

“…T h e effects of diabetes on collagen synthesis have been studied in many organs or tissues, e.g. bone (Harvey & Nakamoto 1988), skin (Fullana et al 1993), periodontal tissue (Oliver & Tervonen 1994), cartilage (Spanheimer 1992), myocardium (Reddi 1988) and arterial tissue (Mariani et al 1990). There is also one study about collagen content in skeletal muscle of diabetic animal (Al-Zaid 1988).…”

The Assessment and Perioperative Management of Patients With Rheumatoid Arthritis

“…Along with a CBCD, a total lymphocyte count (TLC), serum albumin, and retinol binding protein levels are evaluated. [21][22][23][24] Recently, emphasis has been placed on the TLC, with evidence that for every decrease in TLC of 1000 cells/mm, 3 the risk for wound healing difficulties doubles. 24 In the very elderly, low total cholesterol levels indicate overall poor nutritional status with a potential for wound healing problems ( Table 2).…”