Skin Surface Biopsy: An Improved Technique for the Examination of the Horny Layer

Marks, R.; Dawber, R.P.R.

doi:10.1111/j.1365-2133.1971.tb06853.x

Cited by 273 publications

(120 citation statements)

References 6 publications

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“…Öğrencilere enfestasyon ile ilişkisi olabileceği düşünülen cinsiyet, deri tipi, yüzde krem ve ortak havlu kullanılıp kullanılmadığı soruları soruldu. Ardından demodekslerin bulunduğu derinin korneum tabakasında folikül içeriğinin tamamen toplanmasını sağlamak ve cm 2 'deki akar sayısını saptamak için Marks ve Dawber'in standart yüzeyel deri biyopsisi yöntemi uygulandı 15,19 . Uygulama için örnek alınacak cildin temiz ve kuru olmasına dikkat edilerek mikroskop lamının üzerine bir damla siyanoakrilat içeren yapıştırıcı damlatıldı.…”

Section: Gereç Ve Yöntemunclassified

The prevalance of demodex species in faculty of health science students

Özdemir¹,

Özer²,

Özdemir³

et al. 2015

TURKDERM

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Objective: Demodex folliculorum and D. brevis are acari that can be found in hair follicles and sebaceous glands of the skin, especially on face of human. This study was aimed to reveal prevalance of Demodex species in faculty of health science students. Methods: Skin surface biopsy was performed in subjects who completed socio-demographic questionnaire. The samples were examined under a microscope for demodex species. Results: In this study, at least one species of demodex was found to be positive in 47.4% of 270 students. Coexistence of D. folliculorum and D. brevis was observed in most of the subjects (50.8%). In all positive cases, the presence of only Demodex folliculorum or brevis was found in 29.7% and 19.5% respectively. There was no relationship between variables and demodex prevalence. Conclusion: This study showed that demodex species infestation is a common but an ignored health problem in faculty of health science students. GirişBiyolojik sınıflandırma sistemine göre Demodeks soyu; Arachnida sınıfı, Prostigmata takımı ve Demodicidae ailesinde yer alır 1,2 . Bu soya ait Demodeks follikulorum ve D. brevis olmak üzere iki türün, insanlarda parazitik yaşam sürdüğü bilinmektedir 3 . Zorunlu parazit olan bu türler, kıl folikülleri ve derinin yağ salgılayan bezlerinde bulunur 4 . Özellikle alın ve burun bölgelerinde, ayrıca kirpik, kulak ve genital bölgelerde, omuz başı, ön kol gibi yağlı ve kıl kökü barındıran vücut bölgelerinde yerleşir 3,[5][6][7] . İnsandan insana yakın temas, tokalaşma ve öpüşme ile bulaştığı bildirilmiştir 3,7,8 .

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Section: Gereç Ve Yöntemunclassified

The prevalance of demodex species in faculty of health science students

Özdemir¹,

Özer²,

Özdemir³

et al. 2015

TURKDERM

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“…Microcolonies can be seen on skin sampled by the surface-biopsy technique (Marks and Dawber, 1971). Adhesive on a glass slide is used to remove the desquamating epithelium which can then be Gram-stained.…”

Section: Visualisation Of Microcoloniesmentioning

confidence: 99%

Microcolony Size of Microbes on Human Skin

Somerville¹,

Noble²

1973

Journal of Medical Microbiology

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IT has long been clear that large numbers of bacteria reside on the skin surface. Price (1938) found populations of four million bacteria on the hands; Evans et al. (1950) Holt (1971) exploited this apparent discrepancy to estimate the number of cells in each aggregate. He used velvet-pad replicas as a measure of the number of aggregates and a scrubbing technique as a measure of the total bacterial cells, and was able to visualise the skin flora as a series of comparatively large microcolonies. Holt's peak value for a microcolony, 70,000 cells per aggregate, seemed extraordinarily large; we decided to try to confirm Holt's concept and to extend it to anaerobic bacteria. This paper reports the findings. MATERIAL AND METHODSTen healthy persons, five male and five female, were sampled on the forehead, anterior forearm, and periumbilicus. The forehead was chosen as a site likely to carry large numbers of Corynebacterium ucnes, the forearm as a normal skin site liable to environmental contamination, and the periumbilicus as a site unlikely to be exposed to contamination. Contact plates were poured in plastic petri dishes with serum agar (Oxoid Blood Agar Base+5 per cent. horse serum) or thioglycollate tween agar (Difco Thioglycollate Medium without indicator to which was added 1 per cent. Tween 80).The scrubbers were teflon rods in teflon cups and the sampling fluid was phosphate buffer, pH 7.9, containing 0.1 per cent. Triton X-100. They were used for the sampling method described by Williamson, which has been shown to remove 85 per cent. of removable bacteria in the 1st min. After sampling, the fluid was serially diluted in ten-fold steps in buffer with 0.05 per cent. Triton X-100; 0.05-ml drops were inoculated on blood agar (Oxoid Blood Agar Base+S per cent. horse blood) and thioglycollate tween agar. Serum and blood plates were incubated for 2 days at 37°C. Thioglycollate tween plates were incubated for 5 days at 37°C in a MacIntosh and Fildes jar in an atmosphere of hydrogen in the conventional manner.

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“…One of the most convenient ways to collect and examine the infected superficial skin layers is the cyanocrylate skin surface stripping (CSSS), formerly named skin surface biopsy [5][6][7][8][9][10]. In its most widely appreciated context, the SC exerts a major barrier function against microorganisms, ultraviolet radiations, oxidants, and a variety of other toxic xenobiotics.…”

Section: Csss Diagnostic Methodsmentioning

confidence: 99%

Dermatophyte Growth in Glabrous Skin Dermatophytoses of Immuno Compromised Hosts

Ge¹,

C²

2015

J Med Diagn Meth

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Infections remain the most common immediate causes of morbidity in immune compromised hosts. The abated host defences are responsible for an increased susceptibility to various uncommon and opportunistic pathogens, and to a lowered host inflammatory response. Skin lesions develop in up to a third of infections in these patients, and they possibly represent the initial step of a systemic illness. Superficial mycoses are occasionally present under unusual atypical presentations. The resulting clinical infections are at times difficult to diagnose. Some of them are recalcitrant to common conventional therapies. The broad scope of cutaneous manifestations of infections in compromised patients are classified based on four presumed underlying physio-pathologic mechanisms, and on the vast array of potential pathogens so far identified. For mild superficial infections, prompt adequate therapy decreases morbidity and lessens the risk of complications. For more severe involvement, early detection of opportunistic fungal infections helps increasing both the cure and survival rates.

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Skin Surface Biopsy: An Improved Technique for the Examination of the Horny Layer

Cited by 273 publications

References 6 publications

The prevalance of demodex species in faculty of health science students

The prevalance of demodex species in faculty of health science students

Microcolony Size of Microbes on Human Skin

Dermatophyte Growth in Glabrous Skin Dermatophytoses of Immuno Compromised Hosts

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