Skin tape stripping: Which layers of the epidermis are removed?

Sølberg, Julie; Ulrich, Nina H.; Krustrup, Dorrit; Ahlström, Malin G.; Thyssen, Jacob P.; Menné, Torkil; Bonefeld, Charlotte M.; Gadsbøll, Anne-Sofie Østergaard; Balslev, Eva; Johansen, Jeanne Duus

doi:10.1111/cod.13199

Cited by 13 publications

(18 citation statements)

References 10 publications

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“…In general, UDL incorporation to vehicles did not alter their human skin penetration profiles significantly and allowed the fluorescent probes to reach the VED. The dispersion in those results was typical of these techniques, 10,18 mainly as a consequence of the intraindividual variation also previously described 23 …”

Section: Discussionsupporting

confidence: 66%

“…The dispersion in those results was typical of these techniques, 10,18 mainly as a consequence of the intraindividual variation also previously described. 23 In view of the results of the human skin penetration assays in Figures 3 and 4, the distribution of the CAL signal in comparison with R-PE could suggest that incorporation of UDL in TW, BCE, CC, BGE, and GC could favor the release of some aqueous content from the core of the UDL. For pig skin, this effect was only seen when UDLs were incorporated in CC.…”

Section: Discussionmentioning

confidence: 95%

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Comparative skin penetration profiles of formulations including ultradeformable liposomes as potential nanocosmeceutical carriers

Izquierdo

Lillo

Bucci

et al. 2020

J of Cosmetic Dermatology

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Background Ultradeformable liposomes are promising carriers for cosmeceutical actives as they can be loaded with molecules of different polarities, and they present unique penetration properties. Aims While those features have already been tested, we wanted to know whether their special penetration properties could be maintained after incorporation in diverse cosmetic vehicles, including commercial products already in the market. Methods Ultradeformable liposomes loaded with a lipophilic and a hydrophilic fluorescent probe were prepared by lipid film resuspension, followed by extrusion and incorporation to different vehicles and commercial products. Penetration was determined in human and pig skin by incubation, with the Saarbrücken penetration model, followed by the recovery of the probes or by fluorescence microscopy. Results The incorporation of ultradeformable liposomes to cosmetic vehicles did not alter their penetration in most of the cases for human skin explants. Pig skin penetration presented significant differences compared with human explants. Conclusions Ultradeformable liposomes could be useful as versatile cosmeceutical carriers in final product formulations.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 95%

Comparative skin penetration profiles of formulations including ultradeformable liposomes as potential nanocosmeceutical carriers

Izquierdo

Lillo

Bucci

et al. 2020

J of Cosmetic Dermatology

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“…As TS sample the SC, 3,19 most studies focus on upper epidermal pathologies such as atopic eczema (AE) (Table S1; see Supporting Information). However, upper epidermal pathology is not a prerequisite, as exemplified by the use of TS to detect skin cancers 20–22 .…”

Section: Which Dermatoses Can Be Studied From Tape Strips?mentioning

confidence: 99%

“…Studies that assess gene expression need more TS in order to access the granular layer, where intact cells and mRNA reside 3 . Although the number of TS needed to strip the SC varies between individuals, 19 Kim et al . demonstrated, through immunostaining of punch biopsies taken after sequential tape stripping, that 20 TS reaches the granular layer 3 .…”

Section: How Many Tape Strips Should Be Used For Each Patient?mentioning

confidence: 99%

Tape strips in dermatology research*

et al. 2021

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“…It has been shown that due to the stronger adhesion of corneocytes toward the stratum granulosum, the protein content of tapes decreases in parallel with the increase of tape number/depth of penetration (52, 53). Studies examining the depth of skin removed by tape stripping techniques indicate that in case of healthy skin, depending on anatomical location, at least 30 consecutive tapes are needed to remove the stratum corneum (SC) via D-Squame tapes (54, 55). We have confirmed these results by applying 50 sequential half cut D-Squame discs to a healthy skin area.…”

Section: Epidermal Samplingmentioning

confidence: 99%

Non-invasive Approaches for the Diagnosis of Autoimmune/Autoinflammatory Skin Diseases—A Focus on Psoriasis and Lupus erythematosus

et al. 2019

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The traditional diagnostic gold standard for inflammatory skin lesions of unclear etiology is dermato-histopathology. As this approach requires an invasive skin biopsy, biopsy processing and analysis by specialized histologists, it is a resource intensive approach requiring trained healthcare professionals. In many health care settings access to this diagnostic approach can be difficult and outside emergency cases will usually take several weeks. This scenario leads to delayed or inappropriate treatment given to patients. With dramatically increased sensitivity of a range of analysis systems including mass spectrometry, high sensitivity, multiplex ELISA based systems and PCR approaches we are now able to “measure” samples with unprecedented sensitivity and accuracy. Other important developments include the long-term monitoring of parameters using microneedle approaches and the improvement in imaging systems such as optical coherence tomography. In this review we will focus on recent achievements regarding measurements from non-invasive sampling, in particular from plucked hair and skin tape-strips which seem well suited for the diagnosis of lupus erythematosus and psoriatic inflammation, respectively. While these approaches will not replace clinical observation—they can contribute to improved subgroup diagnosis, stratified therapeutic approaches and have great potential for providing molecular and mechanistic insight in to inflammatory skin diseases.

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Skin tape stripping: Which layers of the epidermis are removed?

Cited by 13 publications

References 10 publications

Comparative skin penetration profiles of formulations including ultradeformable liposomes as potential nanocosmeceutical carriers

Comparative skin penetration profiles of formulations including ultradeformable liposomes as potential nanocosmeceutical carriers

Tape strips in dermatology research*

Non-invasive Approaches for the Diagnosis of Autoimmune/Autoinflammatory Skin Diseases—A Focus on Psoriasis and Lupus erythematosus

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