Skin tissue homogenate analysis for ceramide and TGF-1 contents with TGF-1 mRNA expressions after treatment of pomegranate concentrated solution and dried pomegranate concentrate powder in mice

Abstract

The aim of this study was to solve skin moisturizing action mechanism issues of pomegranate concentrated solution (PCS) and dried pomegranate concentrate powder (PCP), at least partially. Materials and methods: In this study, ceramide and TGF-β1 contents with TGF-β1 mRNA expressions were analysis on the skin tissue homogenate samples after 56 days of continuous oral administration of PCS 1, 2, and 4 ml/kg, and PCP 100, 200 and 400 mg/kg. Results: Noticeable and dose-dependent increases of skin TGF-β1 contents and mRNA expressions were demonstrated in all PCP and PCS treated mice as compared with intact vehicle control, but no significant changes on the skin ceramide contents were demonstrated in all PCP and PCS treated mice as compared with intact vehicle control, in the current study. In addition, PCP 200 mg/kg showed similar increases of the skin TGF-β1 contents and mRNA expressions as compared to those of PCS 4 ml/kg. Conclusions: The presented results suggested that in vivo skin moisturizing effects of PCP and PCS after oral administration through up regulation of hyaluronan synthesis demonstrated in our previous results, may be possibly mediated by modulation of TGF-β1 expressions at least partially, without critical influences on the skin ceramide contents.

Effectiveness of Krill Oil in Regulating Skin Moisture

“…Effects of KO on skin TGF-β1 contents and TGF-β1 mRNA expression in skin tissue TGF-β promotes collagen formation through various cellular functions [30], and TGF-β1 is known to stimulate the synthesis of hyaluronan via HAS 1 and HAS 2 mRNA expression [31,32]. KO and L-AA exhibited a significant increase in skin TGF-β1 contents and TGF-β1 mRNA expression compared to the intact control group (Fig.…”