Skin transcriptome profiles associated with coat color in sheep

Fan, Rong; Xie, Jun; Bai, Junming; Wang, Haidong; Xue, Tian; Bai, Rui; Jia, Xiaoyun; Yang, Lei; Song, Yunfei; Herrid, Muren; Gao, Wenjun; He, Xu; Yao, Jianbo; Smith, George W.; Dong, Changsheng

doi:10.1186/1471-2164-14-389

Cited by 76 publications

(72 citation statements)

References 43 publications

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“…García-Gámez et al (2011) proposed that no single keratin gene is responsible for the coat color trait. Fan et al (2013) revealed that high expression of keratin only affected the strength of wool. Other researchers, however, have not found a relationship between keratin genes and fur color formation, and therefore, we did not study these genes.…”

Section: Discussionmentioning

confidence: 99%

“…Other reports have also described a significant correlation between TYRP1 expression and fur color formation (Schmidt-Küntzel et al, 2005;Duchesnes et al, 2009). Fan et al (2013) showed that TYRP1 is the most differentially expressed color formation gene between black and white Sunit sheep. This result is consistent with the experimental results reported here.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome expression profiling of fur color formation in domestic rabbits using Solexa sequencing

et al. 2016

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ABSTRACT. Fur color is an important, genetically determined characteristic of domestic rabbits, and rabbit furs are of great economic value. To investigate the molecular genetics associated with fur color determination in domestic rabbits, we used Solexa-sequencing technology to probe gene expression in dorsal skin tissues sampled from full-sibling Rex rabbits of different colors. The number of expressed genes in each sample was approximately 14,700. Among the top 30 genes and transcription factors with the highest reads per kilobase per million values, the elongation factor-alpha 1 gene was highly expressed in all samples, as were genes of the ribosomal protein and keratin gene families. Compared with the chinchilla (C) Rex rabbit control sample, the numbers of genes in the black (B) and white (W) rabbit samples were 1809 and 460, respectively, and the number of common differentially expressed genes was 257. Clustering analysis of these 257 genes revealed that 32 were up-regulated in sample B and down- regulated in sample W. Of these 32 genes, we identified some that are related to fur formation, including Tyrosinase-related protein 1 (TYRP1) and Tyrosinase (TYR), as well as genes with unknown functions. Quantitative real-time polymerase chain reaction was used to verify the expression patterns of those genes. The findings are expected to provide reference for the further study of fur color formation in rabbits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptome expression profiling of fur color formation in domestic rabbits using Solexa sequencing

et al. 2016

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“…Fan et al found that 49 known coat color genes are expressed in sheep skin, of which 13 genes were highly expressed in black sheep skin. These upregulated genes encode components of melanosomes and their precursors such as dopachrome tautomerase (DCT), MATP, tyrosinase (TYR), and tyrosinase related protein 1 (TYRP1) [26].…”

Section: Discussionmentioning

confidence: 99%

Distribution and expression of SLC45A2 in the skin of sheep with different coat colors

Wang

et al. 2016

Folia Histochem Cytobiol.

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Introduction. To investigate whether the membrane-associated transporter protein SLC45A2 is differentially expressed in the skin of sheep with different coat colors and to determine its correlation with coat color establishment in sheep. Material and methods. The expression of SLC45A2 in sheep skin samples with different coat colors was qualitatively and quantitatively analyzed by PCR amplification, RT-PCR, immunohistochemical staining and Western blotting. Results. A 193-bp SLC45A2 CDS sequence was successfully amplified from sheep skin samples with diverse coat colors. RT-PCR analysis revealed that SLC45A2 mRNA was expressed in all sheep skin samples tested, with relative expression levels of 512.74 ± 121.51 in black skin, 143.38 ± 119.31 and 1.36 ± 0.09 in black dots and white dots of piebald skin, respectively, and 1.02 ± 0.23 in white skin (p < 0.01**). Positive SLC45A2 protein bands were also detected in all skin samples by Western blot analysis, with relative expression levels of 0.85 ± ± 0.17** in black skin, 0.60 ± 0.05** and 0.34 ± 0.07 in black dots and white dots of piebald skin, respectively, and 0.20 ± 0.05 in white skin (p < 0.01**). Immunohistochemical assays revealed that SLC45A2 was expressed in the hair follicle matrix, the inner and outer root sheath, and the dermal papilla in the skin tissues with different coat colors. These patterns were quantified by optical density (OD) analysis, which yielded relative expression levels of 0.23 ± 0.11 in black skin, 0.19 ± 0.09 and 0.10 ± 0.03 in black dots and white dots of piebald skin, respectively, and 0.08 ± 0.01 in white skin (p < 0.05*). Conclusion. SLC45A2 is detectably expressed in sheep skin of all coat colors, though at significantly different levels. SLC45A2 may participate in the establishment of coat color by regulating the synthesis and trafficking of melanin.

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“…According to the results of Fan et al (2013), there were upregulated genes encoding transcription factors regulating mRNA expression (e.g. MITF) in white skin tissue, whereas in black skin tissue several novel genes were detected.…”

Section: High-throughput Genetic Techniquesnext-generation Sequencingmentioning

confidence: 99%

“…This might exclude this gene as having an effect on spotted phenotype formation. Fan et al (2013) analysed skin transcriptomes from white and black merino sheep. The authors obtained over 100 million raw reads from both skin colour types using the Illumina sequencing technology.…”

Section: High-throughput Genetic Techniquesnext-generation Sequencingmentioning

confidence: 99%

Genetic background of coat colour in sheep

et al. 2018

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Abstract. The coat colour of animals is an extremely important trait that affects their behaviour and is decisive for survival in the natural environment. In farm animal breeding, as a result of the selection of a certain coat colour type, animals are characterized by a much greater variety of coat types. This makes them an appropriate model in research in this field. A very important aspect of the coat colour types of farm animals is distinguishing between breeds and varieties based on this trait. Furthermore, for the sheep breeds which are kept for skins and wool, coat/skin colour is an important economic trait. Until now the study of coat colour inheritance in sheep proved the dominance of white colour over pigmented/black coat or skin and of black over brown.

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Skin transcriptome profiles associated with coat color in sheep

Cited by 76 publications

References 43 publications

Transcriptome expression profiling of fur color formation in domestic rabbits using Solexa sequencing

Transcriptome expression profiling of fur color formation in domestic rabbits using Solexa sequencing

Distribution and expression of SLC45A2 in the skin of sheep with different coat colors

Genetic background of coat colour in sheep

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