This study aims to confirm the anti-inflammatory activities of acemannan and
aloesin, which have been studied for various efficacies at various mixed sample
ratios. The mixed samples were mixed at a ratio of 1:1 (AA-1), 1:2 (AA-2), 1:3
(AA-3), 2:1 (AA-4), and 3:1 (AA-5). Seven samples were evaluated for their
cytotoxic ability on macrophages, and the results showed that all cell viability
was over 90% at a concentration of 100 μg/mL. First, due to the NO
production inhibitory activity, a better inhibitory effect was achieved when
using a mixed sample rather than a single material. Afterward, the activity of
inhibiting the production of PGE2, TNF-α, and IL-6 was
confirmed using a mixed sample. It was confirmed that AA-2 had the best
inhibitory activity on producing PGE2, TNF-α, and IL-6 rather
than AA-1, AA-3, AA-4, and AA-5. For this reason, experiments were conducted
using AA-2 to determine the protein expression levels of iNOS and COX-2, which
are inflammation-related proteins. It was confirmed that AA-2 inhibited iNOS and
COX-2 protein expression by 25.01% and 27.27%, respectively,
compared to the LPS-alone treatment group. In conclusion, the mixed sample of
acemannan and aloesin is judged to have anti-inflammatory activity and can
potentially to be used as a functional material.