Skingineering II: transplantation of large-scale laboratory-grown skin analogues in a new pig model

Abstract

We present a novel and valid pig model permitting both transplantation of large autologous, laboratory-engineered skin analogues and also keeping the site of intervention undisturbed for at least three postoperative weeks. Hence, the model will be used for experiments testing whether such large skin analogues can restore near-normal skin, particularly in the long term. If so, clinical application can be envisioned.

Collagen hydrogels strengthened by biodegradable meshes are a basis for dermo-epidermal skin grafts intended to reconstitute human skin in a one-step surgical intervention

“…joint areas) (van der Veen et al ., ). Due to its advantageous properties, collagen type I proved to be an ideal matrix for skin tissue engineering in preclinical models (Böttcher‐Haberzeth et al ., ; Braziulis et al ., ; Faraj et al ., ; Kinikoglu et al ., ; Schiestl et al ., ). This is in contrast to collagen sponges, such as Integra Dermal Regeneration Template ® (Schiestl et al ., ; Stiefel et al ., ) and Matriderm ® (Ryssel et al ., ; van Zuijlen et al ., ), into which and onto which it is difficult, if not impossible, to seed and grow skin cells.…”

A novel system for expansion and delivery of human keratinocytes for the treatment of severe cutaneous injuries using microcarriers and compressed collagen

“…Others have reported the use of plastic compression of collagen hydrogels for the treatment of cutaneous defects. However, these studies involved culturing keratinocytes on the scaffolds with the aim of producing living skin equivalents or bioengineered skin analogues, which included the three‐dimensional (3D) growth of keratinocytes into stratified layers (Ananta et al ., ; Braziulis et al ., ; Schiestl et al ., ). In the present study, the collagen scaffolds are used as a cell delivery device rather than a tissue engineered skin replacement, with the aim of retaining the microcarriers in place on the wound bed, which, as was shown previously, can lead to a superior wound healing outcome in vivo compared with cells applied in suspension (Eldardiri et al ., ).…”

Tissue engineering of skin: human tonsil-derived mesenchymal cells can function as dermal fibroblasts

“…At first, only epidermal sheets, such as autologous cultured epidermal autografts (CEA), were used for coverage, but functional and esthetic results were unsatisfying because of inconsistent graft take, susceptibility to infection, and graft contracture [4][5][6][7][8][9][10]. With the addition of dermal fibroblasts to skin substitutes, a more stable skin construct with better functional and esthetic results has been created [11][12][13]. Fibroblasts produce extracellular matrix components to strengthen the dermal compartment and interact with cells of the epidermal compartment including keratinocytes and melanocytes.…”