Skirmishing MDR strain of Candida albicans by effective antifungal CeO₂ nanostructures using Aspergillus terreus and Talaromyces purpurogenus

Abstract

Emerging antibiotics resistance fungal infectionsis a major global health problem and new antifungal formulations are direly needed to fight drug resistant Candida albicans strains. This study is aimed to synthesize effective antifungal nanostructures of cerium oxide (CeO 2 ) using culture filtrates of two common fungal strains Aspergillus terreus and Talaromyces pupureogenus. The fungal strains used in the synthesis were identified by 18S rRNA gene sequencing and deposited to NCBI GenBank with the accession number of MN099077 and MN121629, respectively. The biofabricated CeO 2 NPs were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Pure CeO 2 nanoparticles (NPs) synthesized using Aspergillus terreus culture filtrate were depicted spherical morphology with average size of 28.5 nm. The CeO 2 NPs synthesized using Talaromyces pupureogenus revealed the presence of nanosponges with average size of 21.4 nm. Gas chromatography mass spectrometry of culture filtrates of respective strains indicated the presence of ethanol, 1-propanol and tri-chloromethane in culture filtrate of Aspergillus terreus and with addition of palmitic acid in Talaromyces pupureogenus culture filtrate which may have a function as bio reducers and capping agents. Dose dependent anticandidal activity of CeO 2 NPs using various different concentrations (100, 200, 300, 600 μg ml −1 ) synthesized by both fungal strains was observed by disc diffusion assay against Candida albicansas evidenced by increase in size of zone of inhibitions with increasing concentration of CeO 2 NPs. Further in-vitro and in-vivo experiments are required to access the potential of CeO 2 NPs for controlling Candida albicans strains.

Harnessing endophytic fungi for biosynthesis of selenium nanoparticles and exploring their bioactivities

“…In the last two decades, the emerging of antibiotic resistant microbes have become the major health apprehension (Komal et al 2020 ) where infectious diseases are the primary causes of deaths around the world (Sundararaj et al 2019). As a result, there is an emerging utmost concern to develop new antimicrobial formulations to mitigate these drug-resistant microbes.…”

“…In accordance with our results, the antimicrobial activity of SeNPs against several bacterial and fungal species was well reported (Mosallam et al 2018 ; Srivastava1 and Mukhopadhyay 2013 ; Alagesan and Venugopal 2019 ; Shubharani et al 2019 ; El-Sayed et al 2020a ). Several NPs were reported to induce leakage of genetic materials, minerals, and proteins of cells by their interaction with cell wall or membrane (El-Sayed et al 2020a ; Komal et al 2020 ; Mousa et al 2021 ). As such, they can inhibit the microbial growth by the induction of reactive O 2 species (Dutta et al 2012 ; El-Sayed et al 2020b ; Mousa et al 2021 ).…”

Gamma rays-assisted bacterial synthesis of bimetallic silver-selenium nanoparticles: powerful antimicrobial, antibiofilm, antioxidant, and photocatalytic activities

“…Rising resistance to antibiotics is a severe global health concern, making it urgently necessary to develop new antimicrobial formulations to fight drug-resistant microorganisms. Drugs that utilize NPs as antimicrobial substances have lately gained a lot of attention in the research of microbial drug resistance [ 82 ]. In order to determine the efficiency of the three types of NPs that were synthesized in this work as possible antimicrobials touching various MDR bacterial strains as well as several human and plant harmful fungi, they were investigated.…”

Fungus-mediated synthesis of Se-BiO-CuO multimetallic nanoparticles as a potential alternative antimicrobial against ESBL-producing Escherichia coli of veterinary origin

“…The fungus was characterized through an assessment of its colony morphology and micro-morphological traits, Additionally, it was identified using a molecular approach involving the analysis of the Internal Transcribed Spacer (ITS) gene sequence. The micro-morphological characteristics of the strain were examined after staining with lacto phenol cotton blue and subsequent observation under a light microscope ( Komal et al., 2020 ). The genomic DNA was isolated using the cetyltrimethylammonium bromide (CTAB) technique.…”