Skrining Dan Identifikasi Mikroba Ligninolitik Pada Pengomposan Alami Tandan Kosong Kelapa Sawit

Rupaedah, Bedah; Purwoko, Devit; Safarrida, Anna; Tajuddin, Teuku; Wahid, Abdul; Sugianto, Mahmud; Sudjai, Imam; Suyono, Agus

doi:10.29122/jbbi.v6i1.3237

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…Maka dari itu, peran kapang dan khamir sebagai mikroorganisme yang membantu memecah serat diperlukan oleh maggot. Kapang dan khamir adalah mikroorganisme yang memiliki kemampuan untuk menciptakan enzim selulase dan ligninase untuk memecah komponen selulosa dan lignin (Rupaedah et al, 2019).…”

Section: Pendahuluanunclassified

ISOLASI DAN IDENTIFIKASI KAPANG DAN KHAMIR PADA MEDIA PERTUMBUHAN MAGGOT Black Soldier Fly

Shofa,

Badruzzaman,

Harlia

2024

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Metode biokonversi menggunakan organisme hidup yaitu maggot BSF (Black Soldier Fly) merupakan salah satu metode untuk mengurai bahan organik yang berasal dari limbah organik. Selain maggot terdapat mikroorganisme pengurai yang berperan dalam proses biokonversi, diantaranya yaitu kapang dan khamir. Penelitian ini bertujuan untuk mengetahui jumlah dan peran kapang serta khamir hasil isolasi dan identifikasi yang terdapat pada media pertumbuhan maggot yang berasal dari kombinasi feses sapi potong, endapan susu, dan sampah organik dapur. Metode yang digunakan dalam penelitian ini adalah metode deskriptif. Hasil penelitian menunjukkan bahwa rata-rata jumlah kapang dan khamir sebelum didegradasi oleh maggot lebih tinggi dibandingkan dengan setelah didegradasi oleh maggot. Jenis kapang yang teridentifikasi pada media pertumbuhan maggot adalah Aspergillus sp, Penicillium sp, Cladosporium sp, Rhizopus sp, Trichoderma sp, dan Mucor sp. Jenis khamir yang teridentifikasi pada media pertumbuhan maggot adalah Saccharomyces sp, Zygosaccharomyces sp, dan Trichosporon sp.The bioconversion method using living organisms, namely BSF (Black Soldier Fly) maggots, is one method to break down organic matter derived from organic waste. Besides maggots, there are parser microorganisms that play a role in the bioconversion process, among them are mold amd yeast. This research aims to determine the number and role of molds and yeasts isolated and identified on maggot growth media derived from a combination of beef cattle feces, dairy waste sludge and organic kitchen waste. The method used in this research is descriptive method. The results showed that the average number of molds and yeasts before degradation by maggot was higher than after degradation by maggot. The types of mold identified in the maggot growth media were Aspergillus sp, Penicillium sp, Cladosporium sp, Rhizopus sp, Trichoderma sp, and Mucor sp. The types of yeast identified in the maggot growth media were Saccharomyces sp, Zygosaccharomyces sp, and Trichosporon sp.

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Section: Pendahuluanunclassified

ISOLASI DAN IDENTIFIKASI KAPANG DAN KHAMIR PADA MEDIA PERTUMBUHAN MAGGOT Black Soldier Fly

Shofa,

Badruzzaman,

Harlia

2024

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“…(Kholis et al, 2019) stated, isolate C9 potent to degrade cellulose of biomass of eucalyptus waste. (Rupaedah et al, 2019) reported that the genus of Bacillus can degrade effectively lignin of OPEFB.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Cellulose-Degrading Bacteria from Luwu Timur in Oil Palm Empty Fruits Bunch

Febryanti¹,

Baharuddin²,

Rahayu³

2021

WJC

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The oil palm empty fruit bunch (OPEFB) is one of the solid wastes produced by industrial factories. These bunches are the habitat of cellulolytic bacteria. This study aims to identify the morphology and determine the potential microorganisms in degrading oil palm empty fruit bunches waste. The methods used in this study were isolation and observation of bacterial morphology, bacterial screening, degradation test of empty oil palm fruit bunches waste, and enzyme activity test with DNS method using UV-Vis spectrophotometer at a wavelength of 530 nm. A collection of 14 isolates were obtained from the isolation process. We obtained 14 isolates from the sample, the largest clear zone was shown by isolate S10. The isolate also exhibited the highest cellulose-degrading activity after 7 days incubation period with a degrading percentage of 13.27%. After incubation for 2 days, the morphological characteristics of the bacteria produced have circular and medium-sized colonies, yellow, colonies wave edges and flat colonies, elevation convex and smooth surface. The resulting enzyme activity was 0.1308 U/mL. Therefore, isolate S10 was suspected to have the potential to degrade plastic.

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Newly Isolated Ligninolytic Bacteria and Its Applications for Multiple Dye Degradation

Rahayu,

Mustafa,

Marjani

et al. 2023

Water Air Soil Pollut

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The textile industry is expanding globally and is considered the backbone of the world’s largest source of foreign exchange. The development of the textile industry has caused environmental contamination due to its dye waste, which is complex and very difficult to resolve with chemical and physical treatments. Azo dye is one of the most widely used dyes in textile and other industries. It is one of the significantly toxic dyes, and when released in water bodies, it causes a serious threat to the environment. A bacterial strain having the potential to degrade a variety of azo dyes such as Congo red (CR), methylene blue (MB), Alizarin Red S (AR), and Remazol Brilliant Blue R (RBBR) was isolated from soil samples in the wood weathering area and further identified and characterized. Ligninolytic microorganisms produce laccase enzymes, lignin peroxidase, manganese peroxidase, and other enzymes that can decolorize dye waste from the textile industry. The research phase was qualitative and quantitative tests of ligninolytic bacteria in the decolorization process using several selected synthetic dyes, antagonism tests, and identification of potential bacteria based on 16S rDNA gene sequences. The L11 isolate showed high performance on CR dye of 82.79%, L1 isolate on dye AR of 40.51%, L7 isolate on dye MB of 38.69%, and L8 isolate on RBBR dye of 30.34%. The L11 isolate with the highest potency was identified as Bacillus paramycoides K7.2 with a similarity of 99.71%. After 7 days of incubation, the quantitative test findings are the same as the qualitative test results, with isolate L11 having the largest clear zone on CR, AR, and RBBR dyes.

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Skrining Dan Identifikasi Mikroba Ligninolitik Pada Pengomposan Alami Tandan Kosong Kelapa Sawit

Cited by 3 publications

References 3 publications

ISOLASI DAN IDENTIFIKASI KAPANG DAN KHAMIR PADA MEDIA PERTUMBUHAN MAGGOT Black Soldier Fly

ISOLASI DAN IDENTIFIKASI KAPANG DAN KHAMIR PADA MEDIA PERTUMBUHAN MAGGOT Black Soldier Fly

Isolation of Cellulose-Degrading Bacteria from Luwu Timur in Oil Palm Empty Fruits Bunch

Newly Isolated Ligninolytic Bacteria and Its Applications for Multiple Dye Degradation

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