Skrining Gen Glukosiltransferase (Gtf) Dari Bakteri Asam Laktat Penghasil Eksopolisakarida

Malik, Amarila; Ariestanti, Donna Maretta; Nurfachtiyani, Anandayu; Yanuar, Arry

doi:10.7454/mss.v12i1.316

Cited by 2 publications

(5 citation statements)

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“…Bahan untuk amplifikasi DNA antara lain GoTaq Green PCR Master Mix (Thermo Scientific), water nuclease-free (Thermo Scientific), Primer untuk amplifikasi BAL dari 1 st Base yaitu forward plb16 (5'-AGAGTTTGATCCTGGCTCAG-3') dan primer reverse mlb16 (5'-GGCTGCTGGC-ACGTAGTTAG-3') seperti yang tercantum dalam Malik et al (2009) dan primer spesifik gen gtf dari 1 st Base yaitu primer DegFor 5'-GAYAAYWSNAAYCCNRYNGTNC-3' dan DegRev 5'ADRTCNCCRTARTANAVNYKNG-3 seperti yang tercantum dalam Malik et al (2008). Penanda DNA untuk proses elektroforesis adalah DNA ladder berukuran 100 bp dari Promega (Madison, USA).…”

Section: Bahanunclassified

“…(3) Amplifikasi gen gtf Amplifikasi gen gtf menggunakan sekuen primer DegFor 5'-GAYAAYWSNAAYCCNRYNGTNC-3' dan DegRev 5'ADRTCNCCRTARTANAVNYKNG-3. Siklus PCR meliputi Predenaturasi 95 ⁰C, 5 menit, Denaturasi 95⁰C, 30 detik; Annealing 42 ⁰C, 45 detik dan Elongasi 72 ⁰C, 60 detik, sebanyak 35 siklus dengan amplikon gen gtf berukuran 660 bp (Malik et al, 2008).…”

Section: Metode (1) Reculture Isolat Probiotikunclassified

“…Identifikasi Glukosiltransferase (gtf) Penyandi Eksopolisakarida (Malik et al, 2008). Siklus PCR meliputi Predenaturasi 95 ⁰C, 5 menit, Denaturasi 95 ⁰C, 30 detik; Annealing 42 ⁰C, 45 detik dan Elongasi 72 ⁰C, 60 detik, sebanyak 35 siklus dengan amplikon gen gtf berukuran 660 pb.…”

Section: Prosedur Analisisunclassified

“…Sukrosa adalah substrat donor untuk enzim glikosil hidrolase (GH), yang dinamai glucansucrase (famili GH70) yang mengkatalisasi sintesis ɑ-glukan, dan terletak secara ekstraseluler (Meng et al, 2016;Gangoiti et al, 2018). Gen gtf berfungsi dalam reaksi hidrolisis Sukrosa menjadi monomer-monomer gula yang selanjutnya akan bergabung sehingga membentuk rantai polimer gula (poligula), yaitu EPS (Malik et al, 2008). Keberadaan gen gtf ini memiliki dampak langsung pada unit berulang dari polimer.…”

Section: Prosedur Analisisunclassified

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IDENTIFIKASI GLUKOSILTRANSFERASE (gtf) PENYANDI EKSOPOLISAKARIDA PADA STRAIN Weisella confusa PROBIOTIK ASAL AIR SUSU IBU (ASI)

Anindita

2020

JPA

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Identifikasi gen gtf dan kemampuan produksi EPS digunakan sebagai parameter dalam seleksi isolat probiotik yang memenuhi syarat Generally Recognized as Safe (GRAS). Penelitian ini bertujuan sebagai salah satu upaya dalam memperoleh isolat lokal kandidat probiotik potensial indigenus asal ASI sebagai penghasil EPS. Metode penelitian ini meliputi identifikasi gen gtf menggunakan primer spesifik (DegFor dan DegRev) dan produksi EPS selama fermentasi. Berdasarkan hasil studi, identifikasi keberadaan gen gtf pada 4 strain Weisella confusa asal ASI terdeteksi pada 660 bp dan Weisella confusa strain AS3 memiliki kemampuan menghasilkan EPS tertinggi yaitu 1883 ± 56 mg/L. Sehingga, Weisella confusa kandidat probiotik potensial asal ASI memiliki kemampuan produksi EPS berdasarkan identifikasi molekuler dan produksi EPS selama fermentasi. Kata kunci: Air susu ibu (ASI), Eksopolisakarida, Glukosiltransferase (gtf), Weisella confusa

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Section: Bahanunclassified

Section: Metode (1) Reculture Isolat Probiotikunclassified

Section: Prosedur Analisisunclassified

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IDENTIFIKASI GLUKOSILTRANSFERASE (gtf) PENYANDI EKSOPOLISAKARIDA PADA STRAIN Weisella confusa PROBIOTIK ASAL AIR SUSU IBU (ASI)

Anindita

2020

JPA

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“…The identification and screening of FTF genes and GTF genes in an isolate will have an impact on enzyme variations that play a role in EPS biosynthesis so that the impact will enrich the variations of EPS polymers with unique structures and new functions that have the potential to be developed in use in the pharmaceutical, health and food fields. [11][12][13] LAB exploration from various sources is currently increasing. LAB can be isolated from various foodstuffs for use as probiotics and functional foods such as fruit, 14,15 vegetables 16,17 and fermented foods.…”

Section: Introductionmentioning

confidence: 99%

Lactic acid bacteria as an exopolysaccarides (EPS) producing starter from pakoba fruit (Syzygium sp.), endemic species at Minahasa, North Sulawesi

Lawalata

2023

IJMR

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: Pakoba fruit ( sp.) is one of the medicinal plants of the Minahasa people and is an endemic species in North Sulawesi. Pakoba can only be found in the Minahasa area and very popular among Minahasa people. The taste is sour, making this fruit as the main ingredient to be processed into rojak, sweets, dodol, jam or syrup. Pakoba fruit is widely used for treatment in the simplest way because this fruit contains many bioactive compounds. The study aim to determine the presence of lactic acid bacteria (LAB) in Pakoba fruit and their potential in producing exopolysaccarides.: From the total of 35 producing-acid bacteria, 17 isolates were confirmed as Lactic Acid Bacteria (LAB) isolates with the characteristics of bacill cells, gram positive, catalase negative, non-motile, non-spore forming, gas production, mesophilic, aciduric, can ferment carbohydrates. : Based on Bergey's Manual of Systematic Bacteriology, the seventeen isolates were identified as member of genus. The seventeen isolates also showed the ability to produce exopolysaccharides in the range of 102-1570 mg /L.

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Skrining Gen Glukosiltransferase (Gtf) Dari Bakteri Asam Laktat Penghasil Eksopolisakarida

Cited by 2 publications

References 1 publication

IDENTIFIKASI GLUKOSILTRANSFERASE (gtf) PENYANDI EKSOPOLISAKARIDA PADA STRAIN Weisella confusa PROBIOTIK ASAL AIR SUSU IBU (ASI)

IDENTIFIKASI GLUKOSILTRANSFERASE (gtf) PENYANDI EKSOPOLISAKARIDA PADA STRAIN Weisella confusa PROBIOTIK ASAL AIR SUSU IBU (ASI)

Lactic acid bacteria as an exopolysaccarides (EPS) producing starter from pakoba fruit (Syzygium sp.), endemic species at Minahasa, North Sulawesi

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