Sla1, a<i>Schizosaccharomyces pombe</i>Homolog of the Human La Protein, Induces Ectopic Meiosis when Its C Terminus Is Truncated

Tanabe, Kaori; Ito, Noriko; Wakuri, Tomomi; Ozoe, Fumiyo; Umeda, Makoto; Katayama, Satoshi; Tanaka, Katsunori; Matsuda, Hideyuki; Kawamukai, Makoto

doi:10.1128/ec.2.6.1274-1287.2003

Cited by 21 publications

(24 citation statements)

References 66 publications

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“…In an alternative regulatory pathway, Pat1 can be inhibited by a truncated version of Sla1, an ortholog of the mammalian La protein. 13,14) Overexpression of the cyr1 gene results in sterility because the cAMP level is kept high even under nutrient starved conditions. 5) On the contrary, a cyr1 mutant undergoes sexual development even under nutrient rich conditions.…”

mentioning

confidence: 99%

Involvement of Moc1 in Sexual Development and Survival ofSchizosaccharomyces pombe

Yakura

Ishikura

Adachi

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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confidence: 99%

Involvement of Moc1 in Sexual Development and Survival ofSchizosaccharomyces pombe

Yakura

Ishikura

Adachi

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…Fragments .4 kb were cloned into the low-copy vector pYC11-ars 3002 1 3 (Dubey et al 1994;Tanabe et al 2003) that had been digested with HindIII or SacI. The S. pombe strain SPRN1DA, which lacks ras1 and cannot sporulate, was transformed by this genomic library, grown for 5 days at 30°, and incubated for 2 days at 25°.…”

Section: Isolation Of a Fission Yeast Homolog Of Zds1 And Zds2mentioning

confidence: 99%

“…Pat1 is inhibited by Mei3, whose expression is induced by the pheromone signaling pathway (Li and McLeod 1996). In an alternative regulatory pathway, Pat1 can be inhibited by a truncated version of Sla1, an ortholog of the mammalian La protein (Tanabe et al 2003(Tanabe et al , 2004.…”

mentioning

confidence: 99%

zds1, a Novel Gene Encoding an Ortholog of Zds1 and Zds2, Controls Sexual Differentiation, Cell Wall Integrity and Cell Morphology in Fission Yeast

Yakura¹,

Ozoe²,

Ishida³

et al. 2006

Genetics

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While screening for genes that reverse the sporulation-deficient phenotype of the ras1D diploid Schizosaccharomyces pombe strain, we identified zds1. This gene shares sequence homology with the ZDS1 and ZDS2 genes from Saccharomyces cerevisiae, which appear to be involved in multiple cellular events. Expression of Zds1 in ras1D diploid cells elevated their sporulation rate from 0.3 to 11.2%. Expression of the Zds1 C-terminal region increased the sporulation rate further (to 21.9%) while introduction of the Zds1 N-terminal region had no effect. zds1 expression did not induce sporulation in strains with mutations in genes participating in the downstream MAP kinase cascade. The zds1-disrupted strain is sensitive to CaCl 2 , and this effect is suppressed by the C-terminal region of Zds1. The growth of the zds1D strain is markedly inhibited by cold temperatures, while its viability decreased in the stationary phase. Moreover, the zds1D strain is round in shape and very sensitive to zymolyase, and its cell wall becomes thicker than that of wild type. Thus, zds1 must be required to maintain cell wall integrity. The Zds1-GFP fusion protein localized to the cytosol, the septum, and the cell cortex. Its localization in the septum was dependent on its C-terminal region. Overexpression of the C-terminal region of Zds1 induced multi-septa and abnormal zygotes. We propose that the C-terminal region is the functional domain of Zds1 while the N-terminal region is a negative regulatory region. Thus, Zds1 is involved in multiple cellular events in fission yeast, including sexual differentiation, Ca 21 tolerance, cell wall integrity, viability in the stationary phase, and cell morphology.

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“…11,12) In addition to these functions, we reported that Sla1 has a novel role in meiosis. 13) Sla1ÁC, a truncated form in the C terminus, which was initially obtained from the screening to rescue the ras1 À phenotype, 13,14) induces ectopic haploid meiosis. This type of haploid meiosis is unique in pat1 ts mutants or a strain that expresses mei2-SATA, a conditional activated mutant of mei2.…”

Section: )mentioning

confidence: 99%

Truncated Sla1 Induces Haploid Meiosis through the Pat1-Mei2 System in Fission Yeast

Tanabe

Tanaka

Matsuda

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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Sla1, aSchizosaccharomyces pombeHomolog of the Human La Protein, Induces Ectopic Meiosis when Its C Terminus Is Truncated

Abstract: Sla1 is a

Cited by 21 publications

References 66 publications

Involvement of Moc1 in Sexual Development and Survival ofSchizosaccharomyces pombe

Involvement of Moc1 in Sexual Development and Survival ofSchizosaccharomyces pombe

zds1, a Novel Gene Encoding an Ortholog of Zds1 and Zds2, Controls Sexual Differentiation, Cell Wall Integrity and Cell Morphology in Fission Yeast

Truncated Sla1 Induces Haploid Meiosis through the Pat1-Mei2 System in Fission Yeast

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