SLAMF1 is required for TLR4-mediated TRAM-TRIF–dependent signaling in human macrophages

Yurchenko, Mariya; Skjesol, Astrid; Ryan, Liv; Richard, Gabriel; Kandasamy, Richard K.; Wang, Ninghai; Terhorst, Cox; Husebye, Harald; Espevik, Terje

doi:10.1083/jcb.201707027

Cited by 48 publications

(63 citation statements)

References 55 publications

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“…In a previous transcriptomic study, we were intrigued by the strong upregulation of SLAMF1 gene expression in human mo‐DCs treated with B. abortus cyclic β1,2‐glucan (CβG; Degos et al, ; Table S1). Because SLAMF1 overexpression follows binding of E. coli outer membrane proteins OmpC and OmpF to macrophages (Berger et al, ; Yurchenko et al, ) and Omp25 modulates human mo‐DC activation upon Brucella infection (Billard et al, ), we explored whether B. abortus with or without Omp25‐controlled murine SLAMF1 protein levels in bone marrow‐derived DCs (BMDCs) in vitro. BMDCs were challenged for 24 hr with B. abortus wild type (Ba WT) or a Omp25‐defective mutant ( Ba ∆ omp25 ) or its complemented mutant strain that stably re‐expressed Omp25 (Ba ∆ omp25 pBBR4 omp25 ) or mock‐treated or treated with E. coli LPS or CβG, as reported (Martirosyan et al, ; Zhao et al, ) and phenotypic investigation was done by flow cytometry (Figure 1a ) .…”

Section: Resultsmentioning

confidence: 99%

“…Recruitment of this cascade may alternatively be impaired. As SLAMF1 is essential for TLR4‐mediated TRAM‐TRIF‐dependent signalling in E. coli ‐infected human macrophages (Yurchenko et al, ), another possibility is that its engagement by Omp25 affects the recruitment of other signalling receptor(s) or adapter protein(s). Further investigations will decipher how the engagement of the SLAMF1 receptor by Brucella Omp25 controls NF‐κB nuclear translocation in DCs and define the transduction pathways involved and their interplay.…”

Section: Discussionmentioning

confidence: 99%

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Omp25‐dependent engagement of SLAMF1 byBrucella abortusin dendritic cells limits acute inflammation and favours bacterial persistence in vivo

Degos

Hysenaj

González-Espinoza

et al. 2020

Cellular Microbiology

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The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25‐dependent engagement of SLAMF1 by B. abortus limits NF‐κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro‐inflammatory cytokine secretion and impairs DC activation. The Omp25‐SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Omp25‐dependent engagement of SLAMF1 byBrucella abortusin dendritic cells limits acute inflammation and favours bacterial persistence in vivo

Degos

Hysenaj

González-Espinoza

et al. 2020

Cellular Microbiology

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“…We aimed to investigate the effects of various concentrations and duration of incubation with PMA on the differentiation of THP-1 monocytic cells to macrophage-like cells. We chose three commonly used differentiation protocols for the analysis based on previous studies (7, 12, 27). Light microscopy analysis revealed changes in PMA induced morphology, including increased cellular adhesion and spread morphology.…”

Section: Resultsmentioning

confidence: 99%

“…The network properties were calculated using NetworkAnalyzer and visualized in Cytoscape using betweenness centrality and degree parameters. The entire networks were further subjected to clustering to identify significant sub-clusters using the MCODE app (v1.5.1) (27) in Cytoscape. The parameters used for clustering included degree cutoff of 2, node score cutoff of 0.2, k -core of 2, and Max.…”

Section: Methodsmentioning

confidence: 99%

Dose-dependent phorbol 12-myristate-13-acetate-mediated monocyte-to-macrophage differentiation induces unique proteomic signatures in THP-1 cells

Pinto

Kim

Subbannayya

et al. 2020

Preprint

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1, 2). Monocytes in the blood circulation migrate to the site of infection/inflammation and 48 differentiate into macrophages for effective host defense, tissue remodeling, and repair (3). 49Furthermore, macrophages exhibit a high level of plasticity, depending on their local 50 microenvironment, specialized functions and varied phenotype acquired (1, 4). 51Several models are employed to study the mechanisms of immune-modulation in monocytes 52 and macrophages during inflammatory diseases. The most frequently used include primary 53 peripheral blood mononuclear cells (PBMCs) and monocyte cell lines. However, due to donor-54 to-donor variations and technical disparities involved in handling of PBMCs in vitro, the 55 human leukemia monocytic cell line, THP-1, is widely accepted and used as a 56 monocyte/macrophage model (5, 6). Several studies have indicated that THP-1 cells can be 57 differentiated into macrophage-like cells using phorbol-12-myristate-13-acetate (PMA), 58 which markedly resembles PBMC monocyte-derived macrophages (MDMs) in cytokine 59 production, metabolic and morphological properties, including differential expression of 60 macrophage surface markers such as CD14, CD11b (ITGAM) and scavenger receptors-CD163, 61 MSR1, and SCARB2 (5, 7-10). Nonetheless, depending on the parameters of the differentiation 62 protocol employed, such as the concentration (ranging from 5 to 400 ng/mL), and duration of 63 incubation (1 to 5 days) with PMA; the degree of differentiation and the functional changes 64 may vary significantly (5,(11)(12)(13)(14)(15). 65At the molecular level, multiple proteins including growth factors, antigenic markers, 66 chemokine-receptors, cytokines, and cell adhesion molecules, are known to govern and reflect 67 underlying monocyte-macrophage differentiation processes (16)(17)(18)(19)(20). However, the effect of 68 various differentiation protocols on the cellular proteome and intracellular signaling networks 69 4 during monocyte-to-macrophage differentiation remains poorly understood. Hence, it is crucial 70 to determine the most suitable differentiation conditions when using these cells as model 71 system, as this can significantly impact their response to various innate immune stimuli. 72Quantitative high-resolution mass spectrometry-based proteomic approaches have been widely 73 employed to investigate the proteomes of monocytes and macrophages as well as altered 74 cellular proteomes and complex cellular/biological mechanisms in several biological 75 conditions (21-23). However, to date, no studies have directly compared the proteome changes 76 of the PMA-mediated differentiation process. 77In the present study, we evaluate the effect of three PMA-based differentiation protocols on 78 the changes in the proteome profiles upon THP-1 differentiation using stable isotope dimethyl 79 labeling quantitative proteomics. We demonstrate that various differentiation conditions such 80 as concentration and incubation time, prior to any stimuli, are critical consideration factors for 81 hetero...

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“…While CD150 is not present on the cell surface of monocytes, it is upregulated on activated cells of monocytes/macrophages lineage. At the same time, CD150 is detected in the cytoplasm of human monocytes and macrophages with preferential localization to early recycling compartments [10]. CD150 is also expressed at very low level on immature dendritic cells (DCs) with strong upregulation following maturation stimuli [7,11,12].…”

Section: The Pattern Of Cd150 Expression In Normal and Malignant Hemamentioning

confidence: 99%

SLAMF1/CD150 in hematologic malignancies: Silent marker or active player?

Gordiienko

Shlapatska

Kovalevska

et al. 2019

Clinical Immunology

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SLAMF1/CD150 receptor is a founder of signaling lymphocyte activation molecule (SLAM) family of cell-surface receptors. It is widely expressed on cells within hematopoietic system. In hematologic malignancies CD150 cell surface expression is restricted to cutaneous T-cell lymphomas, few types of B-cell non-Hodgkin's lymphoma, near half of cases of chronic lymphocytic leukemia, Hodgkin's lymphoma, and multiple myeloma. Differential expression among various types of hematological malignancies allows considering CD150 as diagnostical and potential prognostic marker. Moreover, CD150 may be a target for antibody-based or measles virus oncolytic therapy. Due to CD150 signaling properties it is involved in regulation of malignant cell fate decision and tumor microenvironment in Hodgkin's lymphoma and chronic lymphocytic leukemia. This review summarizes evidence for the important role of CD150 in pathogenesis of hematologic malignancies.

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SLAMF1 is required for TLR4-mediated TRAM-TRIF–dependent signaling in human macrophages

Cited by 48 publications

References 55 publications

Omp25‐dependent engagement of SLAMF1 byBrucella abortusin dendritic cells limits acute inflammation and favours bacterial persistence in vivo

Omp25‐dependent engagement of SLAMF1 byBrucella abortusin dendritic cells limits acute inflammation and favours bacterial persistence in vivo

Dose-dependent phorbol 12-myristate-13-acetate-mediated monocyte-to-macrophage differentiation induces unique proteomic signatures in THP-1 cells

SLAMF1/CD150 in hematologic malignancies: Silent marker or active player?

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