SLC2A1 is a Diagnostic Biomarker Involved in Immune Infiltration of Colorectal Cancer and Associated With m6A Modification and ceRNA

Abstract

Background: Overexpression of solute carrier family 2 member 1 (SLC2A1) promotes glycolysis and proliferation and migration of various tumors. However, there are few comprehensive studies on SLC2A1 in colorectal cancer (CRC).Methods: Oncomine, The Cancer Genome Atlas (TCGA), and Gene Expression Omnibus (GEO) databases were used to analyze the expression of SLC2A1 in pan-cancer and CRC and analyzed the correlation between SLC2A1 expression and clinical characteristics of TCGA CRC samples. The expression level of SLC2A1 in CRC was certified by cell experiments and immunohistochemical staining analysis. The Genome Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) analyses of SLC2A1 relative genes were completed by bioinformatics analysis. The correlation between SLC2A1 expression level and CRC immune infiltration cell was analyzed by Tumor IMmune Estimation Resource (TIMER), Gene Expression Profiling Interactive Analysis (GEPIA), and TCGA database. The correlation between SLC2A1 expression level and ferroptosis and m6A modification of CRC was analyzed by utilizing TCGA and GEO cohort. Finally, the possible competing endogenous RNA (ceRNA) networks involved in SLC2A1 in CRC are predicted and constructed through various databases.Results: SLC2A1 is highly expressed not only in CRC but also in many other tumors. ROC curve indicated that SLC2A1 had high predictive accuracy for the outcomes of tumor. The SLC2A1 expression in CRC was closely correlated with tumor stage and progression free interval (PFI). GO, KEGG, and GSEA analysis indicated that SLC2A1 relative genes were involved in multiple biological functions. The analysis of TIMER, GEPIA, and TCGA database indicated that the SLC2A1 mRNA expression was mainly positively associated with neutrophils. By the analysis of the TCGA and GEO cohort, we identified that the expression of SLC2A1 is closely associated to an m6A modification relative gene Insulin Like Growth Factor 2 MRNA Binding Protein 3 (IGF2BP3) and a ferroptosis relative gene Glutathione Peroxidase 4 (GPX4).Conclusion: SLC2A1 can be used as a biomarker of CRC, which is associated to immune infiltration, m6A modification, ferroptosis, and ceRNA regulatory network of CRC.

Coexpression network analysis of human candida infection reveals key modules and hub genes responsible for host-pathogen interactions

“…SLC2A1 belongs to a glucose transporter family and has been reported to be associated with HCC ( Kim et al, 2017 ). SLC2A1 is also essential to IRI during liver transplantation ( Huang et al, 2019b ) and a diagnostic biomarker for colorectal cancer (CRC) ( Liu et al, 2022 ). In CRC, the SLC2A1 gene infiltrates the CD4 + T cell, neutrophil, dendritic cells, and B cells ( Liu et al, 2022 ).…”

“…SLC2A1 is also essential to IRI during liver transplantation ( Huang et al, 2019b ) and a diagnostic biomarker for colorectal cancer (CRC) ( Liu et al, 2022 ). In CRC, the SLC2A1 gene infiltrates the CD4 + T cell, neutrophil, dendritic cells, and B cells ( Liu et al, 2022 ). Candidiasis is one of the risk factors for Oral squamous cell carcinoma (OSCC).…”

KLF2 is a clinical diagnostic and treatment biomarker of breast cancer

“…The study also employed the “Correlation” module to examine the expression correlation between KLF2 and immune infiltration marker genes. This comprehensive analysis provides important insights into the role of KLF2 in cancer immune infiltration and may offer novel therapeutic strategies for cancer treatment ( Liu et al, 2022 ).…”

Ferroptosis: Reviewing CRC with the Third Eye

“…They validated that SLC2A1 was strongly expressed in CRC and connected to a series of regulatory networks for example ferroptosis, which makes it a potential diagnostic biomarker. 31 Peng et al explored a ferroptosis-related gene (FRG), Metallothionein-1G ( MG1T ), and demonstrated that CRC patients’ immune responses may be impacted by decreased levels of MG1T, which already showed a worse prognosis in CRC patients. 32 There are also some other FRGs screened from databases that are closely related to ferroptosis in CRC, for example, CYBB, YAP1 , 33 NFS1 , 34 TFAP2C, ALOX12, NOS2 , 35–37 NOX4 37 , 38 and so on.…”