Sleep Deprivation Distinctly Alters Glutamate Transporter 1 Apposition and Excitatory Transmission to Orexin and MCH Neurons

Briggs, Chantalle; Hirasawa, Michiru; Semba, Kazue

doi:10.1523/jneurosci.2179-17.2018

Cited by 39 publications

(26 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This role of astrocytes as energy substrate is critical for the activity of orexin neurons. Perisomatic appositions expressing glutamate transporter 1, the main astrocytic transporter which plays a key role in glutamatergic transmission, decrease around orexin neurons and increase around MCH neurons after sleep deprivation in the rat ( Briggs et al, 2018 ).…”

Section: Which Mechanism/s For Diurnal Regulation Of Orexin Neurons?mentioning

confidence: 99%

Daily Fluctuation of Orexin Neuron Activity and Wiring: The Challenge of “Chronoconnectivity”

Azeez

Gallo

Cristino³

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

In the heterogeneous hub represented by the lateral hypothalamus, neurons containing the orexin/hypocretin peptides play a key role in vigilance state transitions and wakefulness stability, energy homeostasis, and other functions relevant for motivated behaviors. Orexin neurons, which project widely to the neuraxis, are innervated by multiple extra- and intra-hypothalamic sources. A key property of the adaptive capacity of orexin neurons is represented by daily variations of activity, which is highest in the period of the animal’s activity and wakefulness. These sets of data are here reviewed. They concern the discharge profile during the sleep/wake cycle, spontaneous Fos induction, peptide synthesis and release reflected by immunostaining intensity and peptide levels in the cerebrospinal fluid as well as postsynaptic effects. At the synaptic level, adaptive capacity of orexin neurons subserved by remodeling of excitatory and inhibitory inputs has been shown in response to changes in the nutritional status and prolonged wakefulness. The present review wishes to highlight that synaptic plasticity in the wiring of orexin neurons also occurs in unperturbed conditions and could account for diurnal variations of orexin neuron activity. Data in zebrafish larvae have shown rhythmic changes in the density of inhibitory innervation of orexin dendrites in relation to vigilance states. Recent findings in mice have indicated a diurnal reorganization of the excitatory/inhibitory balance in the perisomatic innervation of orexin neurons. Taken together these sets of data point to “chronoconnectivity,” i.e., a synaptic rearrangement of inputs to orexin neurons over the course of the day in relation to sleep and wake states. This opens questions on the underlying circadian and homeostatic regulation and on the involved players at synaptic level, which could implicate dual transmitters, cytoskeletal rearrangements, hormonal regulation, as well as surrounding glial cells and extracellular matrix. Furthermore, the question arises of a “chronoconnectivity” in the wiring of other neuronal cell groups of the sleep-wake-regulatory network, many of which are characterized by variations of their firing rate during vigilance states.

show abstract

Section: Which Mechanism/s For Diurnal Regulation Of Orexin Neurons?mentioning

confidence: 99%

Daily Fluctuation of Orexin Neuron Activity and Wiring: The Challenge of “Chronoconnectivity”

Azeez

Gallo

Cristino³

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…They surround synapses, respond non-linearly to neurotransmitters, and via several different mechanisms (e.g. metabolism, neurotransmitter uptake, and gliotransmission), modulate neuronal activity (3)(4)(5)(6). This confers upon these cells the ability to integrate and respond to changes in neurons, providing a feedback mechanism to regulate neuronal activity (3)(4)(5)7).…”

Section: Introductionmentioning

confidence: 99%

A role for astroglial calcium in mammalian sleep

Ingiosi

Hayworth

Harvey

et al. 2019

Preprint

View full text Add to dashboard Cite

Mammalian sleep is characterized by dramatic changes in neuronal activity, and waking neuronal activity is thought to increase sleep need. Changes in other brain cells (glia) across the natural sleep-wake cycle and their role in sleep regulation are comparatively unexplored. We show that sleep is also accompanied by large changes in astroglial activity as measured by intracellular calcium concentrations in unanesthetized mice. These changes in calcium vary across different vigilance states and are most pronounced in distal astroglial processes. We find that reducing intracellular calcium in astrocytes impaired the homeostatic response to sleep deprivation. Thus, astroglial calcium changes dynamically across vigilance states and is a component of the sleep homeostat.One Sentence Summary: Astroglial calcium concentrations vary with sleep and wake, change after sleep deprivation, and mediate sleep need.

show abstract

“…Our studies are also limited to the fly model, and testing vertebrate models is desirable in order to determine whether the observed changes in neural-glial interactions are phylogenetically conserved. Recent studies demonstrated sleep-dependent changes in the astrocyte glutamate transporter, GLT1, apposition to hypothalamic neurons in mice [25]. However, these studies did not conclude whether the changes were due to a redistribution of GLT1 protein in astrocytes, or to structural changes of astrocyte processes (e.g., extension/retraction) onto these neurons.…”

Section: Limitationsmentioning

confidence: 95%

Sleep pressure regulates mushroom body neural-glial interactions in Drosophila

Vanderheyden¹,

Dongen²,

Frank³

et al. 2019

Matters Select

View full text Add to dashboard Cite

Sleep is a behavior that exists broadly across animal phyla, from flies to humans, and is necessary for normal brain function. Recent studies in both vertebrates and invertebrates have suggested a role for glial cells in sleep regulatory processes. Changes in neural-glial interactions have been shown to be critical for synaptic plasticity and circuit function. Here, we wanted to test the hypothesis that changes in sleep pressure alters neural-glial interactions. In the fruit fly, Drosophila melanogaster, sleep is known to be regulated by mushroom body (MB) circuits. We used the technique GFP Reconstitution Across Synaptic Partners (GRASP) to test whether changes in sleep pressure affect neural-glial interactions between MB neurons and astrocytes, a specialized glial cell type known to regulate sleep in flies and mammals. The MB-astrocyte GRASP signal was reduced after 24 h of sleep deprivation, whereas the signal returned to baseline levels following 72 h of recovery. Social enrichment, which increases sleep drive, similarly reduced the MB-astrocyte GRASP signal. We did not observe any changes in the MB-astrocyte GRASP signal over time-of-day, or following paraquat exposure or starvation. These data suggest that changes in sleep pressure are linked to dynamic changes in neural-glial interactions between astrocytes and neuronal sleep circuits, which are not caused by normal rest-activity cycles or stressors.

show abstract

Sleep Deprivation Distinctly Alters Glutamate Transporter 1 Apposition and Excitatory Transmission to Orexin and MCH Neurons

Cited by 39 publications

References 97 publications

Daily Fluctuation of Orexin Neuron Activity and Wiring: The Challenge of “Chronoconnectivity”

Daily Fluctuation of Orexin Neuron Activity and Wiring: The Challenge of “Chronoconnectivity”

A role for astroglial calcium in mammalian sleep

Sleep pressure regulates mushroom body neural-glial interactions in Drosophila

Contact Info

Product

Resources

About