Sleep disruption and its effect on lymphocyte redeployment following an acute bout of exercise

Ingram, Lesley A; Simpson, Richard J.; Malone, Eva; Florida-James, Geraint

doi:10.1016/j.bbi.2014.12.018

Cited by 27 publications

(26 citation statements)

References 33 publications

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“…The main finding of this study is that T ANG cells increase in the peripheral circulation in response to an acute bout of exercise, and subsequently leave the circulation during the early stages of exercise recovery, mirroring the classic biphasic response of T cells to exercise (Simpson et al 2006;Turner et al 2010;Ingram et al 2015). This is in contrast to the study by Lansford et al (2016), who found no changes in T ANG cells in response to acute exercise; however, their study did not report changes in circulating numbers, but proportional changes (percentage of CD3 + T cells).…”

Section: Discussionmentioning

confidence: 62%

“…Aliquots of 0.5 × 10 6 isolated peripheral blood mononuclear cells, as counted using a haemocytometer, were labelled with monoclonal antibodies anti-CD3-APC (clone SK7, IgG 1 ), anti-CD31-FITC (clone WM59, IgG 1 ), anti-CD4 (clone RPA-T4, IgG 1 )/anti-CD8-PE (clone HIT8a, IgG 1 ) and anti-CXCR4-PE-Cy5 (clone 12G5, IgG 2a ; all from BD Biosciences, San Jose, CA, USA) and were allowed to incubate for 45 min at 4°C in the dark prior to flow cytometric analysis. Subanalyses of CD4 + and CD8 + T cells were performed because these cells show differing levels of mobilization in response to exercise (Ingram et al 2015). Immediately prior to flow cytometric enumeration, 500 μl (PBS-BSA) was added to the samples.…”

Section: Blood Sampling and Lymphocyte Phenotypingmentioning

confidence: 99%

“…Changes in blood volume were accounted for by using known measures of haematocrit and haemoglobin obtained from automated haematology analysis (XS 1000i; Sysmex, UK; Dill & Costill, 1974;Ingram et al 2015).…”

Section: Flow Cytometrymentioning

confidence: 99%

“…The T ANG cells were identified as CD3 + CD31 + cells (Hur et al 2007;Kushner et al 2010), with subfraction analyses of CD4 + (CD3 + CD4 + CD31 + ) and CD8 + (CD3 + CD8 + CD31 + ) also performed. Subanalyses of CD4 + and CD8 + T cells were performed because these cells show differing levels of mobilization in response to exercise (Ingram et al 2015). As yet, it is unknown whether these cell subfractions exhibit differing levels of pro-angiogenic activity.…”

Section: Blood Sampling and Lymphocyte Phenotypingmentioning

confidence: 99%

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A 10 km time trial running bout acutely increases the number of angiogenic T cells in the peripheral blood compartment of healthy males

Ross

Tormey

Ingram

et al. 2016

Experimental Physiology

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What is the central question of the study? Are CD31 angiogenic T (T ) cells preferentially mobilized in response to acute exercise? What is the main finding and its importance? Our study reveals that T cells are redistributed into the circulation in response to acute strenuous exercise, but to a lesser extent than CD31 T cells. Of the T cells mobilized, T cells expressing CXCR4 show greater redistribution compared with CXCR4 T cells. Stromal-derived factor 1-α does not appear to play a role in the redistribution of T cells expressing CXCR4. The results suggest that a single bout of strenuous exercise might provide a short vasculogenic window, which could benefit the vascular system by redistributing CD31 T cells. CD31 T cells have been documented to possess vasculogenic properties and have been termed 'angiogenic T cells' (T cells). No study to date has fully characterized the effect of acute exercise on T cells. Twelve male participants aged 24-45 years undertook a running 10 km time trial, with peripheral blood samples taken before, immediately after and 1 h postexercise for quantification of T cells and subsequent CXCR4 cell surface expression by flow cytometry. The T cells demonstrated a 102% increase in number in the peripheral circulation immediately postexercise compared with pre-exercise levels, followed by a large egress (50%) from the circulation in total T cells 1 h postexercise. This was due to changes in both CD4 and CD8 T cells, with CD8 T cells displaying greater ingress (123%) and egress (52%) compared with CD4 T cells (ingress, 83%; egress, 37%). The cell surface expression intensity of CXCR4 was affected only on CD8 T cells, with a significant increase in cell surface expression immediately postexercise versus pre-exercise levels. The CD31 T cells displayed greater redistribution than CD31 T cells (119 versus 102%). CXCR4-expressing T cells showed greater response to acute exercise than CXCR4 cells, which was accompanied by large changes in CXCR4 ligand SDF-1α. The results show that acute exercise increases T cells in the circulation in response to an acute exercise stressor. Additionally, CXCR4 cell surface expression appears to be increased in response to exercise, which may result from the direct upregulation of CXCR4 on the T-cell surface or could be due to CD31 T cells being redistributed into the blood expressing greater levels of CXCR4.

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Section: Discussionmentioning

confidence: 62%

Section: Blood Sampling and Lymphocyte Phenotypingmentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

Section: Blood Sampling and Lymphocyte Phenotypingmentioning

confidence: 99%

See 2 more Smart Citations

A 10 km time trial running bout acutely increases the number of angiogenic T cells in the peripheral blood compartment of healthy males

Ross

Tormey

Ingram

et al. 2016

Experimental Physiology

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“…Previous studies have also observed redistribution of T ANG cells into the circulation with a single bout of exercise, in both young and older men (Ross et al 2016(Ross et al , 2018. This T-cell response is not uncommon, as many T-cell subsets increase in circulation in response to acute exercise stimuli, such as naive T cells (T NA € IVE ), cytotoxic T cells (T CYTOTOXIC ), and regulatory T cells (T REG ) (Brown et al 2014;Spielmann et al 2014;Ingram et al 2015;Clifford et al 2017). In addition to moving into the circulation in response to exercise, these T cells are thought to egress from the circulation within minutes of exercise cessation.…”

Section: Introductionmentioning

confidence: 91%

Older men display elevated levels of senescence-associated exercise-responsive CD28^nullangiogenic T cells compared with younger men

et al. 2018

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Aging is associated with elevated cardiovascular disease risk. As a result of aging, endothelial dysfunction develops, partly due to a reduction in vascular regenerative ability. CD31+ T cells (angiogenic T cells; TANG) possess highly angiogenic capabilities; however, these cells are significantly reduced in older populations. In addition, older populations possess significantly higher senescent and highly differentiated T‐cell levels in circulation, and these are reported to be highly exercise responsive. We investigated whether older adults display greater levels of circulating senescent (CD28null) TANG cells and whether these cells were more exercise responsive than CD28+ TANG cells. Young (18–25 years; n = 9) and older (60–75 years; n = 10) healthy men undertook a 30‐min cycling bout at 70% trueV˙O2peak, with circulating TANG cells (CD3+ CD31+ CD28+/null; including CD4+ and CD8+ subsets) measured preexercise, postexercise, and 1 h post exercise by flow cytometry. Older adults displayed reduced basal levels of TANG cells (mean ± SEM: 410 ± 81 vs. 784 ± 118 cells·μL, P = 0.017), despite a greater proportion of these cells being CD28null (26.26 ± 5.08 vs. 13.36 ± 2.62%, P = 0.044). Exercise significantly increased the circulating number of TANG cells in both young and older men. However, in older men alone, exercise preferentially mobilized CD28null CD8+ TANG cells compared with CD28+ TANG cells (time × phenotype interaction: P = 0.022; Δ74 ± 29 vs. Δ27 ± 15 cells·μL, P = 0.059), with no such difference observed between these phenotypes in the young population. In conclusion, this is the first study to demonstrate that despite observing lower circulating numbers of TANG cells, older adults display greater levels of senescent TANG cells in comparison with younger individuals, and these cells are more exercise responsive than CD28+ TANG cells. Lower number of circulating TANG and greater levels of senescent‐associated CD28null TANG may contribute to greater CVD risk with advancing age.

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Circadian Regulation of Sleep: From Genes to Circuits

Hameed,

Barber

2024

Genetics of Sleep and Sleep Disorders

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Sleep disruption and its effect on lymphocyte redeployment following an acute bout of exercise

Cited by 27 publications

References 33 publications

A 10 km time trial running bout acutely increases the number of angiogenic T cells in the peripheral blood compartment of healthy males

A 10 km time trial running bout acutely increases the number of angiogenic T cells in the peripheral blood compartment of healthy males

Older men display elevated levels of senescence-associated exercise-responsive CD28^nullangiogenic T cells compared with younger men

Circadian Regulation of Sleep: From Genes to Circuits

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Sleep disruption and its effect on lymphocyte redeployment following an acute bout of exercise

Cited by 27 publications

References 33 publications

A 10 km time trial running bout acutely increases the number of angiogenic T cells in the peripheral blood compartment of healthy males

A 10 km time trial running bout acutely increases the number of angiogenic T cells in the peripheral blood compartment of healthy males

Older men display elevated levels of senescence-associated exercise-responsive CD28nullangiogenic T cells compared with younger men

Circadian Regulation of Sleep: From Genes to Circuits

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Older men display elevated levels of senescence-associated exercise-responsive CD28^nullangiogenic T cells compared with younger men