Current study is aiming to find a more suitable behavioral fatigue index for Air Traffic Controllers(ATC) using PVT test and fatigue self-assessment questionnaires as tools, and gives these tests before and after ATC duty. Through PVT test and self-assessment questionnaire, this finding indicates hit rate and hit response time (the first 20% trials) of PVT can be potential fatigue index since we found first 2min hit response time of air traffic controller(ATC) after duty is significantly longer than before duty, together with the hit rate after duty is lower than before duty. 8 items in Stanford Sleepiness Scale(SSS) and Visual Analogy Scale(VAS) also show significant difference in self assessment on fatigue. ATCs think themselves are much more fatigue after duty than before duty.Besides, ATCs in regional control area show significant fatigue effect than those in terminal control area, indicating different work load in two different working areas. Those ATC work at night especially in midnight show much more significant fatigue than those work in the daytime showed by longer first 20% hit response time. In sum, hit rate and first 20% (first 2min) hit response time can be effective fatigue indexes of ATC.