Sll0939 is induced by Slr0967 in the cyanobacterium Synechocystis sp. PCC6803 and is essential for growth under various stress conditions

Uchiyama, Junji; Asakura, Ryosuke; Moriyama, Atsushi; Kubo, Yuko; Shibata, Yosuke; Yoshino, Yuka; Tahara, Hiroko; Matsuhashi, Ayumi; Sato, Shusei; Nakamura, Yasukazu; Tabata, Satoshi; Ohta, Hisataka

doi:10.1016/j.plaphy.2014.02.007

Cited by 10 publications

(8 citation statements)

References 37 publications

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“…Before starting the evolution experiments under acid stress, the P strain was grown at two different pH levels in BG-11 (pH 8.0 or pH 5.5) to distinguish the phenotypic and genetic changes occurring during the adaptive evolution to BG-11 medium from those that occurred due to acid stress. As shown in our previous study, the P strain growth was remarkably slower at pH 6.0 than that at pH 8.0 Tahara et al 2012;Uchiyama et al 2014). The P strain did not grow at pH 5.5 (Fig.…”

Section: Acid-adapted Strain Isolationsupporting

confidence: 67%

“…Synechocystis 6803 has many acid-inducible genes according to the previous studies (Ohta et al 2005;Uchiyama et al 2012;Tahara et al 2012;Zhang et al 2012;Uchiyama et al 2014). However, these genes do not include the genes with SNPs detected in the A.A. strains.…”

Section: Transcriptional Analysis Of Snp Genes and Acid-inducible Genesmentioning

confidence: 99%

“…A previous study showed that acid stress tolerance genes are involved in other type of stress tolerance Uchiyama et al 2014). Thus, acid-adapted strains may exhibit tolerance to other forms of stress in addition to acidity.…”

Section: Phenotypic Analysis Of Acid-adapted Strains In Various Stresmentioning

confidence: 99%

“…DNA microarray analysis of Synechocystis 6803 cells showed that acid stress induced the expression of putative stress-related proteins such as chaperones [slr0093 (dnaJ), sll1514 (hspA), and sll0170 (dnaK)], regulatory factors [sll0306 (sigB) and sll2012 (sigD)], and proteins with unknown functions (Ohta et al 2005). More recently, we reported that sphR, sltA1, slr0967, and sll0939 are essential for growth under acid stress Tahara et al 2012;Uchiyama et al 2014). These results indicate that the phenotypic analysis of mutant cells combined with DNA microarrays can be used to study stress tolerance mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

et al. 2015

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Experimental evolution is a powerful tool for clarifying phenotypic and genotypic changes responsible for adaptive evolution. In this study, we isolated acid-adapted Synechocystis sp. PCC 6803 (Synechocystis 6803) strains to identify genes involved in acid tolerance. Synechocystis 6803 is rarely found in habitants with pH < 5.75. The parent (P) strain was cultured in BG-11 at pH 6.0. We gradually lowered the pH of the medium from pH 6.0 to pH 5.5 over 3 months. Our adapted cells could grow in acid stress conditions at pH 5.5, whereas the parent cells could not. We performed whole-genome sequencing and compared the acid-adapted and P strains, thereby identifying 11 SNPs in the acid-adapted strains, including in Fo F1-ATPase. To determine whether the SNP genes responded to acid stress, we examined gene expression in the adapted strains using quantitative reverse-transcription polymerase chain reaction. sll0914, sll1496, sll0528, and sll1144 expressions increased under acid stress in the P strain, whereas sll0162, sll0163, slr0623, and slr0529 expressions decreased. There were no differences in the SNP genes expression levels between the P strain and two adapted strains, except for sll0528. These results suggest that SNPs in certain genes are involved in acid stress tolerance in Synechocystis 6803.

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Section: Acid-adapted Strain Isolationsupporting

confidence: 67%

Section: Transcriptional Analysis Of Snp Genes and Acid-inducible Genesmentioning

confidence: 99%

Section: Phenotypic Analysis Of Acid-adapted Strains In Various Stresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

et al. 2015

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“…These differences most probably result from different cultivation conditions and sampling times in the different studies. Many other salt-stimulated proteins have no functional annotations, however, the proteins Slr0967, Slr2019, Sll0528, and Sll0947 were all also implicated in multiple stress responses of Synechocystis 6803 (Uchiyama et al, 2014; Matsuhashi et al, 2015; Lei et al, 2014; Galmozzi et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Integrative analysis of the salt stress response in cyanobacteria

Klaehn

Mikkat

Riediger

et al. 2021

Preprint

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Microorganisms evolved specific acclimation strategies to thrive in environments of high or fluctuating salinities. Here, salt acclimation in the model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by integrating transcriptomic, proteomic and metabolomic data. A dynamic reorganization of the transcriptome and proteome occurred during the first hours after salt shock, e.g. involving the upregulation of genes to activate compatible solute biochemistry balancing osmotic pressure. The massive accumulation of glucosylglycerol then had a measurable impact on the overall carbon and nitrogen metabolism. In addition, we observed the coordinated induction of putative regulatory RNAs and of several proteins known for their involvement in other stress responses. Overall, salt-induced changes in the proteome and transcriptome showed good correlations, especially among the stably up-regulated proteins and their transcripts. We define an extended salt stimulon comprising proteins directly or indirectly related to compatible solute metabolism, ion and water movements, and a distinct set of regulatory RNAs involved in post-transcriptional regulation. Our comprehensive data set provides the basis for engineering cyanobacterial salt tolerance and to further understand its regulation.

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Slr1909, a Novel two‐component Response Regulator Involved in Acid Tolerance in Synechocystis sp. PCC 6803

Chen

Ren

Wang

et al. 2016

Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria

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Sll0939 is induced by Slr0967 in the cyanobacterium Synechocystis sp. PCC6803 and is essential for growth under various stress conditions

Cited by 10 publications

References 37 publications

Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

Integrative analysis of the salt stress response in cyanobacteria

Slr1909, a Novel two‐component Response Regulator Involved in Acid Tolerance in Synechocystis sp. PCC 6803

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