Slow and ultra-rapid freezing protocols for cryopreserving roe deer (Capreolus capreolus) epididymal sperm collected at different times of year

Santiago-Moreno, J.; Castaño, C.; Bóveda, Paula; Mejía, O.; Velázquez, Rosario; Martínez-Fresneda, Lucía; Flores-Gil, V.N.; Marcos-Beltrán, J.L.; González-Guirado, A. M.; Esteso, M.C.; Toledano-Dı́az, A.

doi:10.1007/s10344-021-01468-4

Cited by 6 publications

(4 citation statements)

References 45 publications

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“…Conversely, in deer species spermatogenesis is completely arrested T A B L E 1 Sperm characteristics and main effects of treatment for each sperm variable in Dwarf bucks treated with repeated immunization with Improvac (group RI: one dose every 6 months during 3 consecutive years, six doses total, n = 3), single immunization with Improvac (group SI: first dose and booster, n = 3), and nontreated bucks (group CON, n = 3). outside the breeding season, when plasma testosterone concentrations fall at basal levels (Gosch & Fischer, 1989;Santiago-Moreno et al, 2021). On the other hand, in male giraffes immunized against GnRH every 3 months testosterone concentrations decreased immediately after the second injection and remained at basal levels at least for 19 months (Moresco et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, testosterone concentrations fall and sperm quality declines outside the rutting season without complete abolishment of spermatogenic activity in wild bovid species (Coloma et al, 2011). Conversely, in deer species spermatogenesis is completely arrested outside the breeding season, when plasma testosterone concentrations fall at basal levels (Gosch & Fischer, 1989; Santiago‐Moreno et al, 2021). On the other hand, in male giraffes immunized against GnRH every 3 months testosterone concentrations decreased immediately after the second injection and remained at basal levels at least for 19 months (Moresco et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Single or repeated immunization against GnRH fails to completely abolish spermatogenesis in dwarf bucks (Capra hircus)

Giriboni

Martínez‐Nevado²,

García³

et al. 2022

Zoo Biology

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In both captive wildlife and production animals is important to develop strategies for population control. Immunization against GnRH is an easy and inexpensive immunocastration method that reduces the concentration of testosterone and decreases sperm quality. However, its effectiveness depends on the species and repetition of the treatment. This study aimed to compare the effectiveness of a single treatment (initial immunization plus a booster with Improvac) vs repeated treatment (six doses of Improvac) to inhibit testicular function and maintain the contraceptive status during long periods in bucks. Three Dwarf bucks (Capra hircus) received two doses of Improvac, the first on Week 0, and the booster 4 weeks later (single immunization, group SI) while three Dwarf bucks received one dose of Improvac every 6 months during 3 consecutive years (repeated immunization, group RI). The other three Dwarf bucks remained untreated (control bucks, group CON). Bucks from RI had a greater decrease in scrotal circumference, testosterone concentration, male odor intensity, and sperm quality than SI bucks. However, there were no differences between SI and CON bucks in any of the variables studied. Overall, repeated treatment of Improvac decreased the testicular function of Dwarf bucks, although did not produce complete infertility. However, the repetition of the treatment produced more intensive negative effects, indicating that the strength of the effects of Improvac is rapidly lost in bucks.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Single or repeated immunization against GnRH fails to completely abolish spermatogenesis in dwarf bucks (Capra hircus)

Giriboni

Martínez‐Nevado²,

García³

et al. 2022

Zoo Biology

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“…They detected no significant correlations between the cryo-resistance for motility, viability, membrane functional integrity, or acrosome integrity and the sperm head area, for either epididymal or ejaculated sperm (125). In another recent study, Santiago-Moreno et al (126) explored slow and ultrarapid freezing protocols for cryopreserving roe deer epididymal sperm collected at different times of year. No significant differences were seen between the slow-frozen-thawed and the ultrarapid-frozenthawed sperm in terms of percentage of viable sperm or sperm with morphological abnormalities (126).…”

Section: Recent Developments In Sperm Handling Cryopreservation and B...mentioning

confidence: 99%

“…In another recent study, Santiago-Moreno et al (126) explored slow and ultrarapid freezing protocols for cryopreserving roe deer epididymal sperm collected at different times of year. No significant differences were seen between the slow-frozen-thawed and the ultrarapid-frozenthawed sperm in terms of percentage of viable sperm or sperm with morphological abnormalities (126). Ultrarapid freezing could be particularly promising for marsupials, in which sperm cryopreservation has been very challenging so far (83).…”

Section: Recent Developments In Sperm Handling Cryopreservation and B...mentioning

confidence: 99%

Recent Progress in Spermatology Contributing to the Knowledge and Conservation of Rare and Endangered Species

Comizzoli

Holt

2022

Annu. Rev. Anim. Biosci.

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There is a remarkable diversity in the animal kingdom regarding mechanisms underlying the production, maturation, structure, and function of sperm cells. Spermatology studies contribute to the knowledge of species diversity and also provide information about individual or population fitness. Furthermore, this fundamental research is required before collected spermatozoa can be used for conservation breeding, including assisted reproduction and cryobanking. This article aims to ( a) review the most recent knowledge on sperm morphology and function in wild animal species, ( b) analyze how this knowledge can be used to save species in their natural habitat or ex situ, and ( c) propose future scientific directions in wildlife spermatology that could positively impact animal conservation. Variations in sperm structure and performance within and between species have multiple origins and significance. This collective body of knowledge enables the design and implementation of conservation strategies and action plans that integrate several disciplines. Expected final online publication date for the Annual Review of Animal Biosciences, Volume 10 is February 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Sperm freezability is neither associated with the expression of aquaporin 3 nor sperm head dimensions in dromedary camel (Camelus dromedarius)

O’Brien¹,

Malo²,

Castaño³

et al. 2022

Theriogenology

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Slow and ultra-rapid freezing protocols for cryopreserving roe deer (Capreolus capreolus) epididymal sperm collected at different times of year

Cited by 6 publications

References 45 publications

Single or repeated immunization against GnRH fails to completely abolish spermatogenesis in dwarf bucks (Capra hircus)

Single or repeated immunization against GnRH fails to completely abolish spermatogenesis in dwarf bucks (Capra hircus)

Recent Progress in Spermatology Contributing to the Knowledge and Conservation of Rare and Endangered Species

Sperm freezability is neither associated with the expression of aquaporin 3 nor sperm head dimensions in dromedary camel (Camelus dromedarius)

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