Slow excitatory synaptic potentials recorded from neurones of guinea‐pig submucous plexus.

Surprenant, Annmarie

doi:10.1113/jphysiol.1984.sp015249

Cited by 103 publications

(120 citation statements)

References 31 publications

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“…These are more rapid in onset and decay, lasting 10 -30 s, and may be due to an increase in non-specific cation conductance [134]. This latter conclusion is controversial as some authors have reported slow EPSPs of this type associated with small decreases in membrane conductance, rather than the increases implied by this conclusion [7,93,117,125]. The third class of slow EPSP has been termed "sustained slow postsynaptic excitation" and is evoked by prolonged trains of relatively low frequency stimulation [2,25].…”

Section: Slow Epsps Their Underlying Conductances and Their Transmitmentioning

confidence: 59%

“…Second, there have been many reports that slow EPSPs in S/uniaxonal myenteric neurons can involve reductions in K + conductance or increased neuronal input resistance [15,43,59,93,125]. Third, many slow EPSPs in submucosal S/uniaxonal neurons are associated with increased input resistance and reverse at about the K + equilibrium potential [7,117]. Indeed, it has been reported that both conductance increase and conductance decrease slow EPSPs are seen in the same submucosal neurons [51].…”

Section: Slow Epsps Their Underlying Conductances and Their Transmitmentioning

confidence: 99%

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Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

Gwynne

Bornstein²

2007

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Digestion and absorption of nutrients and the secretion and reabsorption of fluid in the gastrointestinal tract are regulated by neurons of the enteric nervous system (ENS), the extensive peripheral nerve network contained within the intestinal wall. The ENS is an important physiological model for the study of neural networks since it is both complex and accessible. At least 20 different neurochemically and functionally distinct classes of enteric neurons have been identified in the guinea pig ileum. These neurons express a wide range of ionotropic and metabotropic receptors. Synaptic potentials mediated by ionotropic receptors such as the nicotinic acetylcholine receptor, P2X purinoceptors and 5-HT 3 receptors are seen in many enteric neurons. However, prominent synaptic potentials mediated by metabotropic receptors, like the P2Y 1 receptor and the NK 1 receptor, are also seen in these neurons. Studies of synaptic transmission between the different neuron classes within the enteric neural pathways have shown that both ionotropic and metabotropic synaptic potentials play major roles at distinct synapses within simple reflex pathways. However, there are still functional synapses at which no known transmitter or receptor has been identified. This review describes the identified roles for both ionotropic and metabotropic neurotransmission at functionally defined synapses within the guinea pig ileum ENS. It is concluded that metabotropic synaptic potentials act as primary transmitters at some synapses. It is suggested identification of the interactions between different synaptic potentials in the production of complex behaviours will require the use of well validated computer models of the enteric neural circuitry.

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Section: Slow Epsps Their Underlying Conductances and Their Transmitmentioning

confidence: 59%

Section: Slow Epsps Their Underlying Conductances and Their Transmitmentioning

confidence: 99%

Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

Gwynne

Bornstein²

2007

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“…Submucosal plexus preparations were obtained from the small intestine of guinea-pigs (150-250 g); methods of tissue preparation and intracellular recording from submucosal neurones were as detailed previously (Surprenant, 1984). Tissues were superfused at 3-4 ml min-' with a physiological solution of the following composition (mM): NaCl 126, NaH2PO4 1.2, MgCl2 1.2, CaCl2 2.5, KCl 5, NaHCO3 25, glucose l1; gassed with 95% 02 and 5% CO2.…”

Section: Methodsmentioning

confidence: 99%

Vasoconstriction of guinea‐pig submucosal arterioles following sympathetic nerve stimulation is mediated by the release of ATP

Evans

Surprenant

1992

British J Pharmacology

151

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The nature of the transmitter mediating vasoconstriction of guinea-pig submucosal arterioles following sympathetic nerve stimulation was studied.2 Prazosin (0.1 pM) abolished the response to exogenously applied phenylephrine (I pM) but had no effect on constrictions of submucosal arterioles evoked by nerve stimulation (100 pulses at 10 Hz). 3 Vasoconstrictions and excitatory junction potentials elicited by nerve stimulation were potentiated by idazoxan (0.1 pM).4 Following reserpine treatment, catecholamine fluorescence was absent in submucosal arterioles but nerve-evoked vasoconstrictions were unaltered. 5 Vasoconstrictions and excitatory junction potentials recorded in response to sympathetic nerve stimulation, as well as constrictions evoked by exogenously applied ATP (3 tiM), were abolished by the P,-purinoceptor antagonist, suramin (100 liM). Suramin had no effect on the vasoconstriction in response to noradrenaline (3 jM), or the nicotinic excitatory postsynaptic potentials (e.p.s.ps) and noradrenergic inhibitory postsynaptic potentials (i.p.s.ps) recorded from submucosal neurones. 6 We conclude that postjunctional responses of submucosal arterioles following sympathetic nerve stimulation are mediated solely through the activation of P2x-purinoceptors by ATP or a related purine nucelotide. The function of neurally released noradrenaline is to act through prejunctional M2-adrenoceptors to depress transmitter release.

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“…The stimulus threshold for activation of the nicotinic e.p.s.p. is much lower than for activation of the adrenergic inhibitory synaptic potential or the peptidergic slow excitatory synaptic potential (Surprenant, 1984); in all these experiments stimulus parameters were used which elicited only the nicotinic e.p.s.p. The time course of decay of the e.p.s.p.…”

Section: Methodsmentioning

confidence: 99%

“…Methods of dissection, intracellular recording from submucosal neurones, stimulation of interganglionic nerve fibres and optical measurements of arteriolar diameter have been described in detail previously (Surprenant, 1984;Neild et al, 1990 Ltd, 1990 nist; previous studies have shown that cumulative and noncumulative applications of UK 14304 produce similar hyperpolarizations in submucosal neurones (North & Surprenant, 1985;Surprenant & North, 1988). Schild plots were obtained from data on individual neurones and the dissociation equilibrium constants (KDs) and slopes so obtained were then averaged.…”

Section: Methodsmentioning

confidence: 99%

Functional characterization of neuronal pre and postsynaptic α₂‐adrenoceptor subtypes in guinea‐pig submucosal plexus

Shen

Barajas‐López

Surprenant

1990

British J Pharmacology

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1 The a2-adrenoceptors on cell bodies of submucosal neurones, on presynaptic cholinergic nerve terminals innervating submucosal neurones, and on presynaptic sympathetic fibres innervating submucosal arterioles were characterized in functional studies by use of subtype selective ligands. 2 Both membrane hyperpolarization and presynaptic inhibition of nicotinic excitatory synaptic potentials (e.p.s.ps) produced by UK 14304 were similarly antagonized by idazoxan, yohimbine, SKF 104078, WB 4101 and ARC-239. Antagonism was competitive and dissociation equilibrium constants were the same for both effects. 3 Vasoconstriction of submucosal arterioles in response to stimulation of the sympathetic nerves (20 Hz for 2s) was inhibited by UK 14304 and clonidine; concentrations producing half-maximum responses were 6nM and lOnM respectively. Idazoxan, yohimbine, WB 4101 and SKF 104078 antagonized this action, with dissociation constants similar to those for antagonism of the postsynaptic membrane hyperpolarization and presynaptic inhibition of nicotinic e.p.s.ps. 4 Oxymetazoline was a partial agonist when membrane hyperpolarization or presynaptic inhibition of nicotinic e.p.s.ps were measured but a full agonist when presynaptic inhibition of sympatheticallymediated arteriolar vasoconstriction was measured. As an agonist, oxymetazoline produced half maximum responses at 80-120nM; the dissociation constant for oxymetazoline as an antagonist was 130 nM.5 Neither prazosin nor chlorpromazine (up to 30pM) altered any of the three responses to a2-adrenoceptor agonists. 6 It is concluded that oe2-adrenoceptors present on submucosal neuronal cell bodies, on presynaptic cholinergic nerve terminals and on presynaptic sympathetic nerve terminals are the a2A subtype. However, functional characterization of this subtype differs from that provided by ligand binding studies.

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Slow excitatory synaptic potentials recorded from neurones of guinea‐pig submucous plexus.

Abstract: SUMMARY1. Intracellular recordings made from neurones of guinea-pig submucous plexus revealed three types of synaptic input: cholinergic excitatory synaptic potentials (fast e

Cited by 103 publications

References 31 publications

Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

Vasoconstriction of guinea‐pig submucosal arterioles following sympathetic nerve stimulation is mediated by the release of ATP

Functional characterization of neuronal pre and postsynaptic α₂‐adrenoceptor subtypes in guinea‐pig submucosal plexus

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Slow excitatory synaptic potentials recorded from neurones of guinea‐pig submucous plexus.

Abstract: SUMMARY1. Intracellular recordings made from neurones of guinea-pig submucous plexus revealed three types of synaptic input: cholinergic excitatory synaptic potentials (fast e

Cited by 103 publications

References 31 publications

Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

Vasoconstriction of guinea‐pig submucosal arterioles following sympathetic nerve stimulation is mediated by the release of ATP

Functional characterization of neuronal pre and postsynaptic α2‐adrenoceptor subtypes in guinea‐pig submucosal plexus

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Functional characterization of neuronal pre and postsynaptic α₂‐adrenoceptor subtypes in guinea‐pig submucosal plexus