1986
DOI: 10.1128/aem.51.6.1220-1223.1986
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Slow rehydration for detection of Salmonella spp. in feeds and feed ingredients

Abstract: Rehydration and equilibration (4 h) of feeds and feed ingredients at water/sample ratios (vol/wt) of 1.4 to 3.2 did not markedly increase recovery of Salmonella spp. in the slurry when analyzed by standard cultural and direct enrichment methods. Of 143 naturally contaminated samples examined, equilibration increased levels of detection from 106 to 109 positive samples by the standard cultural method and from 103 to 112 by direct enrichment. Results suggest that nonhomogeneous distribution of low incident numbe… Show more

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Cited by 17 publications
(3 citation statements)
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“…Salmonella can survive in feed for prolonged periods of time and contaminate farm animals. In several studies, Salmonella could readily be isolated from several samples that had been stored at room temperature for up to 3 years (D'Aoust, 1977;D'Aoust & Sewell, 1986;Ray, Jezeski, & Busta, 1971;Rayman, D'Aoust, Aris, Maishment, & Wasik, 1979). In another study, broth culture of S. Typhimurium (5.6e9.8 ✕ 10 8 CFU/ml) was added to 70 g of pelleted poultry feed and the samples were stored at 11, 25, and 38 C, with a relative humidity of 68, 51, and 27%, respectively.…”
Section: Contamination In Farm Environments and Persistence Mechanismmentioning
confidence: 99%
“…Salmonella can survive in feed for prolonged periods of time and contaminate farm animals. In several studies, Salmonella could readily be isolated from several samples that had been stored at room temperature for up to 3 years (D'Aoust, 1977;D'Aoust & Sewell, 1986;Ray, Jezeski, & Busta, 1971;Rayman, D'Aoust, Aris, Maishment, & Wasik, 1979). In another study, broth culture of S. Typhimurium (5.6e9.8 ✕ 10 8 CFU/ml) was added to 70 g of pelleted poultry feed and the samples were stored at 11, 25, and 38 C, with a relative humidity of 68, 51, and 27%, respectively.…”
Section: Contamination In Farm Environments and Persistence Mechanismmentioning
confidence: 99%
“…The numbers obtained are comparable to the scarce data that can be found in the literature, estimating levels typically <50 MPN per 100 g or most often <1 MPN per 100 g feed (Smeltzer et al . ; D'Aoust and Sewell ; Sauli et al . ; Binter et al .…”
Section: Discussionmentioning
confidence: 99%
“…However, there are few studies where Salmonella are enumerated in naturally contaminated feed, but numbers as low as <1 MPN per 100 g have been reported (Smeltzer et al . ; D'Aoust and Sewell ). In a more recent study, it was estimated that the level of contamination could be as low as a few CFU per kg of feed (Sauli et al .…”
Section: Introductionmentioning
confidence: 98%