Slr0967 and Sll0939 induced by the SphR response regulator in Synechocystis sp. PCC 6803 are essential for growth under acid stress conditions

Uchiyama, Junji; Asakura, Ryosuke; Kimura, Mayuko; Moriyama, Atsushi; Tahara, Hiroko; Kobayashi, Yuta; Kubo, Yuko; Yoshihara, Toshihiro; Ohta, Hisataka

doi:10.1016/j.bbabio.2012.03.028

Cited by 18 publications

(7 citation statements)

References 31 publications

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“…These two pathways were both reported to be involved in regulating resistance under stress conditions ( Los et al, 2010 ; Foo et al, 2014 ). For example, Uchiyama et al (2012) found that slr1214 encoding two-component response regulator PatA subfamily, may affect acid stress tolerance ( Uchiyama et al, 2012 ). Although many differentially expressed genes were found encoding hypothetical proteins, the results showed that the up-regulated genes were significantly enriched in several functional categories, such as “photosynthesis”, “photosynthesis antenna proteins,” and “two-component system,” while most down-regulated genes were enriched in “pentose phosphate pathway” and “arginine and proline metabolism” ( Supplementary Table S5 ).…”

Section: Resultsmentioning

confidence: 99%

Regulation Mechanism Mediated by Trans-Encoded sRNA Nc117 in Short Chain Alcohols Tolerance in Synechocystis sp. PCC 6803

Pei

Sun

et al. 2018

Front. Microbiol.

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Microbial small RNAs (sRNAs) play essential roles against many stress conditions in cyanobacteria. However, little is known on their regulatory mechanisms on biofuels tolerance. In our previous sRNA analysis, a trans-encoded sRNA Nc117 was found involved in the tolerance to ethanol and 1-butanol in Synechocystis sp. PCC 6803. However, its functional mechanism is yet to be determined. In this study, functional characterization of sRNA Nc117 was performed. Briefly, the exact length of the trans-encoded sRNA Nc117 was determined to be 102 nucleotides using 3′ RACE, and the positive regulation of Nc117 on short chain alcohols tolerance was further confirmed. Then, computational target prediction and transcriptomic analysis were integrated to explore the potential targets of Nc117. A total of 119 up-regulated and 116 down-regulated genes were identified in nc117 overexpression strain compared with the wild type by comparative transcriptomic analysis, among which the upstream regions of five genes were overlapped with those predicted by computational target approach. Based on the phenotype analysis of gene deletion and overexpression strains under short chain alcohols stress, one gene slr0007 encoding D-glycero-alpha-D-manno-heptose 1-phosphate guanylyltransferase was determined as a potential target of Nc117, suggesting that the synthesis of LPS or S-layer glycoprotein may be responsible for the tolerance enhancement. As the first reported trans-encoded sRNA positively regulating biofuels tolerance in cyanobacteria, this study not only provided evidence for a new regulatory mechanism of trans-encoded sRNA in cyanobacteria, but also valuable information for rational construction of high-tolerant cyanobacterial chassis.

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Section: Resultsmentioning

confidence: 99%

Regulation Mechanism Mediated by Trans-Encoded sRNA Nc117 in Short Chain Alcohols Tolerance in Synechocystis sp. PCC 6803

Pei

Sun

et al. 2018

Front. Microbiol.

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“…Synechocystis 6803 has many acid-inducible genes according to the previous studies (Ohta et al 2005;Uchiyama et al 2012;Tahara et al 2012;Zhang et al 2012;Uchiyama et al 2014). However, these genes do not include the genes with SNPs detected in the A.A. strains.…”

Section: Transcriptional Analysis Of Snp Genes and Acid-inducible Genesmentioning

confidence: 94%

Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

et al. 2015

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Experimental evolution is a powerful tool for clarifying phenotypic and genotypic changes responsible for adaptive evolution. In this study, we isolated acid-adapted Synechocystis sp. PCC 6803 (Synechocystis 6803) strains to identify genes involved in acid tolerance. Synechocystis 6803 is rarely found in habitants with pH < 5.75. The parent (P) strain was cultured in BG-11 at pH 6.0. We gradually lowered the pH of the medium from pH 6.0 to pH 5.5 over 3 months. Our adapted cells could grow in acid stress conditions at pH 5.5, whereas the parent cells could not. We performed whole-genome sequencing and compared the acid-adapted and P strains, thereby identifying 11 SNPs in the acid-adapted strains, including in Fo F1-ATPase. To determine whether the SNP genes responded to acid stress, we examined gene expression in the adapted strains using quantitative reverse-transcription polymerase chain reaction. sll0914, sll1496, sll0528, and sll1144 expressions increased under acid stress in the P strain, whereas sll0162, sll0163, slr0623, and slr0529 expressions decreased. There were no differences in the SNP genes expression levels between the P strain and two adapted strains, except for sll0528. These results suggest that SNPs in certain genes are involved in acid stress tolerance in Synechocystis 6803.

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“…Apart from the high temperature, the transcription of HSP genes is induced by hyperosmotic [21] or salt stress [19,22,23], strong light [24], UV-B [25], oxidative stress [14,26], and low pH [27,28]. Only 7 out of 80 genes demonstrate a specific response to heat shock (Table 1, labeled with asterisk).…”

Section: Systemic Analysis Of Cyanobacterial Stress Transcriptomesmentioning

confidence: 99%

Universal Molecular Triggers of Stress Responses in Cyanobacterium Synechocystis

Mironov

Sinetova

Shumskaya

et al. 2019

Life

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Systemic analysis of stress-induced transcription in the cyanobacterium Synechocystis sp. strain PCC 6803 identifies a number of genes as being induced in response to most abiotic stressors (heat, osmotic, saline, acid stress, strong light, and ultraviolet radiation). Genes for heat-shock proteins (HSPs) are activated by all these stresses and form a group that universally responds to all environmental changes. The functions of universal triggers of stress responses in cyanobacteria can be performed by reactive oxygen species (ROS), in particular H2O2, as well as changes in the redox potential of the components of the photosynthetic electron transport chain. The double mutant of Synechocystis sp. PCC 6803 (katG/tpx, or sll1987/sll0755), which is defective in antioxidant enzymes catalase (KatG) and thioredoxin peroxidase (Tpx), cannot grow in the presence of exogenous hydrogen peroxide (H2O2); and it is extremely sensitive to low concentrations of H2O2, especially under conditions of cold stress. Experiments on this mutant demonstrate that H2O2 is involved in regulation of gene expression that responds to a decrease in ambient temperature, and affects both the perception and the signal transduction of cold stress. In addition, they suggest that formation of ROS largely depends on the physical state of the membranes such as fluidity or viscosity. In cyanobacteria, an increase in membrane turnover leads to a decrease in the formation of ROS and an increase in resistance to cold stress. Therefore: (1) H2O2 is the universal trigger of stress responses in cyanobacterial cells; (2) ROS formation (in particular, H2O2) depends on the physical properties of both cytoplasmic and thylakoid membranes; (3) The destructive effect of H2O2 is reduced by increasing of fluidity of biological membranes.

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Slr0967 and Sll0939 induced by the SphR response regulator in Synechocystis sp. PCC 6803 are essential for growth under acid stress conditions

Cited by 18 publications

References 31 publications

Regulation Mechanism Mediated by Trans-Encoded sRNA Nc117 in Short Chain Alcohols Tolerance in Synechocystis sp. PCC 6803

Regulation Mechanism Mediated by Trans-Encoded sRNA Nc117 in Short Chain Alcohols Tolerance in Synechocystis sp. PCC 6803

Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

Universal Molecular Triggers of Stress Responses in Cyanobacterium Synechocystis

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