The upsurge in fungal diseases that cause grapevine wood rot in young and adult plants remains a major concern for the wine sector. So as to study the effectiveness of heat treatments for the sanitization of pruning waste, the thermal death of two inocula of Diplodia seriata fungus-associated to black dead arm disease-was studied in vitro at different temperatures in a dry air incubator, confirming that inhibition increased as a function of temperature and exposure time. First-order and Weibull models were compared to describe D. seriata fungus survival curve kinetics, with high R 2 values (0.923 and 0.930, respectively) and high significances (p<0.001) for both models. According to the first-order model, the time required to reach 100% mortality at 65 °C was 120 min (or 93 min to 95%), whereas for the Weibull model a 90% reduction was attained after 73 min.