SLX4 interacts with RTEL1 to prevent transcription-mediated DNA replication perturbations

Abstract

Back to Main Page1. Please note that only one statement of equally contributing authors is allowed (as well as one statement of joint supervision, if appropriate), so the two present statements have been amalgamated into one for now. If the two pairs of authors need to be diffentiated, a statement can be added to the author contributions section to explain. If you would like to do this, please provide some text to add.The meer fact that two different statements were made was because they were not the same. So they must not be amalgamated.I have added a line in the Author Contribution section.2. First para of main text, sentence beginning "In yeast", please check that "SlX4" is correct. Should it be SLX4?It should be Slx4 with lowercase L and lowercase X 3. Please check your article carefully, coordinate with any co-authors and enter all final edits clearly in the eproof, remembering to save frequently. Once corrections are submitted, we cannot routinely make further changes to the article. OK 4. Note that the eproof should be amended in only one browser window at any one time; otherwise changes will be overwritten.OK 5. Author surnames have been highlighted. Please check these carefully and adjust if the first name or surname is marked up incorrectly. Note that changes here will affect indexing of your article in public repositories such as PubMed. Also, carefully check the spelling and numbering of all author names and affiliations, and the corresponding email address(es). OK 6. You cannot alter accepted Supplementary Information files except for critical changes to scientific content. If you do resupply any files, please also provide a brief (but complete) list of changes. If these are not considered scientific changes, any altered Supplementary files will not be used, only the originally accepted version will be published. OK 7. Para beginning "Here, we unravel" -please check that PCNA has been expanded correctly at first mention. e.Proofing https://eproofing.springer.com/journals_v2/printpage.php?token... 1 of 64 22/04/2020, 15:41 OK 8. Results section, first para, please define YFP at first mention Yellow fluorescent protein (YFP) Figure 1b -please define IgCThis is a typo. It should be IgG in the figure and the following definition added to the legend:IgG: Immunoglobulin G 10. Figure 1c, bottom axes of plots -please check that the jump from 10 h to 12 h is correct (the other time points are in increments of 1 h). Please also check that the labels added to the right of the immunoblots in panel c are correct.All is correct Figure 1 caption -please define HA at first mentionHemagglutinin (HA) Figure 1 -Please check carefully that the caption for panel c is correct as edited to match the new figure.All is OK In the caption to Figure 2g,h -please differentiate (by inserting (g) and (h) what the two panels show.The caption is the same for g and h. The only difference is that the mutations in g are missense mutations while they are nonsense mutations in h. I have changed the caption to:Co-immunoprecipitation of en...

Human RTEL1 associates with Poldip3 to facilitate responses to replication stress and R-loop resolution

“…Likewise, POLδ deficiency also renders cells hypersensitive to TOPOI inhibitors (Tumini et al 2016), raising the possibility that both RTEL1 and POLδ play a critical role in overcoming TOPOI-induced DNA damage during replication. In further support of this concept, two reports published at the time of submission of our revised manuscript suggest that RTEL1 is involved in suppressing replication-transcription conflicts (Takedachi et al 2020;Wu et al 2020) and R-loop accumulation (Wu et al 2020).…”

MutSβ Stimulates Holliday Junction Resolution by the SMX Complex

“…In addition to its interaction with the three nucleases, SLX4 also interacts with proteins involved in mismatch repair (MMR; MSH2, MSH3, and MSH6) and telomere maintenance (TRF2, RTEL1, and SLX4IP), as determined by mass spectrometry (Gonzá lez-Prieto et al, 2015;Svendsen et al, 2009;Takedachi et al, 2020;Wu et al, 2020;Zhang et al, 2019). The MSH proteins form two heterodimeric ATPases, MutSa (MSH2-MSH6) and MutSb (MSH2-MSH3), which are key components of the MMR system that removes errors incorporated during DNA replication (Fishel, 2015).…”

Histone dynamics during DNA replication stress

“…In addition, TRCs and DNA:RNA hybrids activate the Fanconi anemia (FA) DSB pathway to resolve R-loops [ 60 , 61 ]. In line with this mechanism, a recent study showed that SLX4, a tumor suppressor, directs the recruitment of FANCD2 (a critical FA complex member) to RNA polymerase II, and this action is necessary for prevention of TRCs in unstressed cells [ 62 ]. Polycomb group proteins BMI1 and RNF2 were recently revealed to suppress TRCs as well [ 63 ].…”