Small activating RNA induced expression of VHL gene in renal cell carcinoma

Kang, Moo Rim; Park, Ki Hwan; Lee, Chang Woo; Lee, Myeong Youl; Han, Sang‐Bae; Li, Long-Cheng; Kang, Jong Soon

doi:10.1016/j.biocel.2018.02.002

Cited by 15 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NaNO 2 was used to generate a standard curve, and the concentration of nitrite in the medium was determined by measuring optical density at 540 nm [13]. Cell viability was determined by XTT assay as described previously [14]. And it was confirmed that the concentration of compounds used in this study has no significant effect on cell viability.…”

Section: Methodsmentioning

confidence: 79%

Phenazine Derivatives with Anti-Inflammatory Activity from the Deep-Sea Sediment-Derived Yeast-Like Fungus Cystobasidium laryngis IV17-028

et al. 2019

View full text Add to dashboard Cite

Three new phenazine derivatives (1–3), along with known compounds (4–7) of saphenic acid derivatives, were isolated from a deep-sea sediment-derived yeast-like fungus Cystobasidium larynigs collected from the Indian Ocean. The structures of the new compounds (1–3) were determined by analysis of spectroscopic data, semi-synthesis and comparison of optical rotation values. All the isolated compounds (1–7), except for 2, showed nitric oxide (NO) production inhibitory effect against lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells without cytotoxicity at concentrations up to 30 μg/mL. This is the first report on the yeast-like fungus Cystobasidium laryngis producing phenazines and anti-inflammatory activity of 1–7 including saphenic acid (4).

show abstract

Section: Methodsmentioning

confidence: 79%

Phenazine Derivatives with Anti-Inflammatory Activity from the Deep-Sea Sediment-Derived Yeast-Like Fungus Cystobasidium laryngis IV17-028

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Therefore, induction of TTP expression might be a potential therapeutic strategy for BCa. dsRNAs are newly discovered gene activation tools and have been shown to activate the expression of multiple tumor suppressor genes in tumor cells 19,32‐34 . Referring to the previous designs of dsRNAs, 30 we designed and selected six candidate dsRNAs (dsTTP‐275, dsTTP‐547, dsTTP‐676, dsTTP‐699, dsTTP‐830, and dsTTP‐973) targeting promoter of TTP according to the quality scores of dsRNAs (Table 2 and Table ).…”

Section: Resultsmentioning

confidence: 99%

“…By forming a complex with Argonaute 2 (Ago2) protein, dsRNAs can specifically bind to DNA of target promoters based on the base complementary pairing principle, and promote formation of RNA‐induced transcriptional activation (RITA) complex to induce transcription of target genes 17 . Recently, dsRNAs had been shown to reactivate several tumor suppressor genes such as P53, 18 P21, 16,17 and VHL, 19 and significantly inhibited growth and metastasis of cancer cells. With the development of material science, a liposomal nanoparticles loaded dsRNAs named MTL‐CEBPA had been successfully delivered to rat liver and suppressed tumorigenesis of rat hepatocellular carcinoma model via activating CEBPA expression, and the MTL‐CEBPA is now in a phase I clinical trial for patients with advanced cirrhotic hepatocellular carcinoma 20 .…”

Section: Introductionmentioning

confidence: 99%

Tumor suppressing effects of tristetraprolin and its small double‐stranded RNAs in bladder cancer

et al. 2020

View full text Add to dashboard Cite

Bladder cancer (BCa) is a common malignant tumor of urinary system with few treatments, so more useful therapeutic targets are still needed. Antitumor effects of tristetraprolin (TTP) have been explored in many type tumors, but its roles in bladder cancer are still unknown until now. In this study, public expression profiles and tissue microarray analysis showed that TTP mRNA and protein levels decreased in BCa relative to the normal bladder tissue. To explore biological functions of TTP in BCa, 488 TTP target genes, which could be both suppressed and bound by TTP, were identified by comprehensively analyzing publicly available high‐throughput data obtained from Gene Expression Omnibus (GEO). Gene enrichment analysis showed that these genes were enriched in pathways such as cell cycle, epithelial to mesenchymal transition (EMT), and Wnt signaling. Clustering analysis and gene set variation analysis indicated that patients with high expression of TTP target genes had poorer prognosis and stronger tumor proliferation ability relative to the BCa patients with low expression of TTP target genes. In vitro experiments validated that TTP could suppress proliferation, migration, and invasiveness of BCa cells. And TTP could suppress mRNA expression of cyclin‐dependent kinase 1 (CDK1) in BCa cells by target its 3′ UTR. Then, we identified a new small double‐stranded RNA (dsRNA) named dsTTP‐973 which could increase TTP expression in BCa cells, in vivo and in vitro experiments revealed that dsTTP‐973 could suppress aggressiveness of BCa. In conclusion, TTP played a role of tumor suppressor gene in BCa like other tumors, and its dsRNA named dsTTP‐973 could induce TTP expression in BCa and suppress aggressiveness of BCa. With the help of materials science, dsTTP‐973 may become a potential treatment for BCa in the future.

show abstract

“…[7,10] However, little is known about the correlation between NSCLC chemotherapy and VHL gene. [7][8][9][10] The aim of the current study is to investigate the association between Von Hippel-Lindau(VHL) expression and outcomes of platinum-based chemotherapy in NSCLC patient. Specially, we evaluated the expression of VHL in the pathological tissue of NSCLC patients by IHC analysis.…”

Section: Introductionmentioning

confidence: 99%

“…[7] Many researches have pointed out that VHL could in uence the occurrence and metastasis of carcinomas through regulating the HIF pathway. [6][7][8][9] Several studies showed that lack of VHL could cause resistance in the chemotherapy of different carcinomas, including clear-cell renal cell carcinoma (ccRCC) and other solid tumors. [7,10] However, little is known about the correlation between NSCLC chemotherapy and VHL gene.…”

Section: Introductionmentioning

confidence: 99%

VHL Expression Level in the Pathological Tissue is Significantly Associated with Clinical Outcomes of Platinum-Based Chemotherapy in Non-Small Cell Lung Cancer Patients.

Cao

Wang

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

Objective: To investigate the association between expression level of Von Hippel-Lindau(VHL) in pathological tissue and outcomes of platinum-based chemotherapy in non-small cell lung cancer (NSCLC) patients. Methods: The pathological samples of NSCLC patients were obtained for immunohistochemical staining to evaluate the expression level of VHL. Furthermore, their clinical data were collected and prognosis was traced by phone. The correlation between gene expression level and the hematotoxicity was evaluated by chi-square test. The influence of VHL expression level on the risk of hematotoxicity was tested by logistic regression model. The survival curve was plotted by Kaplan-Meier method and the survival rate between the two groups was compared by log-rank test. Results: A total of 110 NSCLC patients were enrolled in this study, the median follow-up time of these patients was 27.5 months. In the whole group, 31 patients had died by the last date of follow-up to get their survival information (Nov.10,2020), with a median survival time of 24.3 months. Though immunohistochemical analysis,we found that 59 patients(53.6%) had weak expression level of VHL or lack of expression in their tumor tissues,while 51 patients(46.4%) presented moderate or high expression. We found that the patients with weak expression of VHL in their carcinoma tissue or lack of expression had more opportunities to occur neutropenia after platinum-based chemotherapy(OR=0.264,95%CI=0.085-0.818,P-value=0.021).And the expression level of VHL was correlated with OS(Logrank test：P-value= 0.007，HR= 4.219,95%CI: 1.75-10.174, P-value=0.001), while not related with DFS(Logrank test：P-value=0.256，HR= 1.334,95%CI:0.642-2.769, P-value=0.440). Conclusion: The expression level of VHL gene in pathological tissue is related with Granulocytosis and leukocytotoxicity after platinum-based chemotherapy in NSCLC patients. It can be used as a biomarker to predict the risk of neutropenia and the prognosis of NSCLC patients.

show abstract

Small activating RNA induced expression of VHL gene in renal cell carcinoma

Cited by 15 publications

References 35 publications

Phenazine Derivatives with Anti-Inflammatory Activity from the Deep-Sea Sediment-Derived Yeast-Like Fungus Cystobasidium laryngis IV17-028

Phenazine Derivatives with Anti-Inflammatory Activity from the Deep-Sea Sediment-Derived Yeast-Like Fungus Cystobasidium laryngis IV17-028

Tumor suppressing effects of tristetraprolin and its small double‐stranded RNAs in bladder cancer

VHL Expression Level in the Pathological Tissue is Significantly Associated with Clinical Outcomes of Platinum-Based Chemotherapy in Non-Small Cell Lung Cancer Patients.

Contact Info

Product

Resources

About