Small-angle scattering of apoferritin in solution

Bielig, Hans‐Joachim; Kratky, O.; Rohns, G.; Wawra, H.

doi:10.1016/s0926-6585(96)90013-8

Cited by 30 publications

(7 citation statements)

References 29 publications

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“…The inner and outer radii of the protein shell determined by SAXS measurements of apo-ferritin without the Ag load are 3.434 ( 0.005 nm and 6.394 ( 0.005 nm, in good agreement with literature data. 49 These values were used to reduce the fit parameters of the Ag-loaded samples. The differential Ag particle number density size distribution is shown in Figure 3.…”

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Silver Ion Incorporation and Nanoparticle Formation inside the Cavity of Pyrococcus furiosus Ferritin: Structural and Size-Distribution Analyses

et al. 2010

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Highly symmetrical protein cage architectures from three different iron storage proteins, heavy and light human ferritin chains (HuHFt and HuLFt) and ferritin from the hyperthemophilic bacterium Pyrococcus furiosus (PfFt), have been used as models for understanding the molecular basis of silver ion deposition and metal core formation inside the protein cavity. Biomineralization using protein cavities is an important issue for the fabrication of biometamaterials under mild synthetic conditions. Silver nanoparticles (AgNPs) were produced with high yields within PfFt but not within HuHFt and HuLFt. To explain the molecular basis of silver incorporation, the X-ray crystal structure of Ag-containing PfFt has been solved. This is the first structure of a silver containing ferritin reported to date, and it revealed the presence of specific binding and nucleation sites of Ag(I) that are not conserved in other ferritin templates. The AgNP encapsulated by PfFt were further characterized by the combined use of different physical-chemical techniques. These showed that the AgNPs are endowed with a narrow size distribution (2.1 +/- 0.4 nm), high stability in water solution at millimolar concentration, and high thermal stability. These properties make the AgNP obtained within PftFt exploitable for a range of applications, in fields as diverse as catalysis in water, preparation of metamaterials, and in vivo diagnosis and antibacterial or tumor therapy.

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Silver Ion Incorporation and Nanoparticle Formation inside the Cavity of Pyrococcus furiosus Ferritin: Structural and Size-Distribution Analyses

et al. 2010

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“…• We refer to summarizing presentations on small-angle scattering: Kratky & Porod (1949), Guinier & Fournet (1955), Beeman et al (1967), Kratky (1963), Brumberger (1968), Alexander (1969). (Bielig et al 1966. ) A.…”

Section: Remarks To the Experimental Methodsmentioning

confidence: 99%

“…There holds (2) Molecular-weight determination Here we only mention the measurements on apoferritin (Bielig et al 1966), as in this case, we dispose of a comparison with other methods. Table 1 shows that the results are satisfying.…”

Section: B the Volume (1) Generalmentioning

confidence: 99%

Recent advances and applications of diffuse X-ray small-angle scattering on biopolymers in dilute solutions

Kratky¹,

Pilz

1972

Quart. Rev. Biophys.

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“…of 75 A, and an outer diam. of 120 A (Harrison, 1964;Bielig et al, 1966), while the iron core may contain as many as 2,000 iron atoms (Fischbach and Anderegg, 1965). The core is strictly paramagnetic, with an average magnetic susceptibility of 5,900 x 10-6 cgs emu/mol iron at 270C (Michaelis et al, 1943).…”

Section: Theorymentioning

confidence: 99%

Ferritin conjugates as specific magnetic labels. Implications for cell separation

Odette¹,

McCloskey²,

Young³

1984

Biophysical Journal

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Concanavalin A coupled to the naturally occurring iron storage protein ferritin is used to label rat erythrocytes and increase the cells' magnetic susceptibility. Labeled cells are introduced into a chamber containing spherical iron particles and the chamber is placed in a uniform 5.2 kG (gauss) magnetic field. The trajectory of cells in the inhomogeneous magnetic field around the iron particles and the polar distributions of cells bound to the iron particles compare well with the theoretical predictions for high gradient magnetic systems. On the basis of these findings we suggest that ferritin conjugated ligands can be used for selective magnetic separation of labeled cells.

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Small-angle scattering of apoferritin in solution

Cited by 30 publications

References 29 publications

Silver Ion Incorporation and Nanoparticle Formation inside the Cavity of Pyrococcus furiosus Ferritin: Structural and Size-Distribution Analyses

Silver Ion Incorporation and Nanoparticle Formation inside the Cavity of Pyrococcus furiosus Ferritin: Structural and Size-Distribution Analyses

Recent advances and applications of diffuse X-ray small-angle scattering on biopolymers in dilute solutions

Ferritin conjugates as specific magnetic labels. Implications for cell separation

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