“…The invariant volume was calculated as in [2]. The values obtained by this method are V = 1600 nm 3 for dimeric holoenzyme (monomeric holoenzyme: V = 790 nm a [7]) and V = 3700 nm 3 for oligomeric core enzyme (monomeric core enzyme: V = 675 nm 3 [4]).…”
Section: Volumementioning
confidence: 99%
“…A model of DNA -dependent RNA polymerase from E. coli has been developed on the basis of neutron and X-ray small angle scattering measurements [1][2][3][4][5][6][7]. These studies were performed in buffers of high ionic strength, in which the enzyme is monomerle.…”
“…The invariant volume was calculated as in [2]. The values obtained by this method are V = 1600 nm 3 for dimeric holoenzyme (monomeric holoenzyme: V = 790 nm a [7]) and V = 3700 nm 3 for oligomeric core enzyme (monomeric core enzyme: V = 675 nm 3 [4]).…”
Section: Volumementioning
confidence: 99%
“…A model of DNA -dependent RNA polymerase from E. coli has been developed on the basis of neutron and X-ray small angle scattering measurements [1][2][3][4][5][6][7]. These studies were performed in buffers of high ionic strength, in which the enzyme is monomerle.…”
“…Each scattering curve was recorded several times with a fixed number (105) of pulses per angle in order to minimize statistical errors. The experimental arrangement and the procedures used for data evaluation were as in [4]. …”
Section: Bill-angle X-ray Scatterirlgmentioning
confidence: 99%
“…After desmearing, the radius of gyration [4], determined from this corrected scattering curve was calculated to be R = 6.67 + 0.1 nm. This value agrees with the value computed from the P(P) function [4]. The intrallarticular distance distribution function was calculated by use of the evaluation program [9].…”
Section: Radius Of Gyration and Maximum Dimensionmentioning
confidence: 99%
“…Here, the structure of holoenzyme (M, 487 000), the arrangement of u on core enzyme, is investigated by small-angle X-ray scattering, considering the results in [4,5] on the structure of core enzyme and CJ factor.…”
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.