Small-animal blood exchange is an emerging approach for systemic aging research

Mehdipour, Melod; Amiri, Payam; Liu, Chao; DeCastro, Jonalyn; Kato, Cameron; Skinner, Colin M.; Conboy, Michael J.; Aran, Kiana; Conboy, Irina M.

doi:10.1038/s41596-022-00731-5

Cited by 9 publications

(6 citation statements)

References 41 publications

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“…In current animal research methods for blood exchange, jugular vein catheterization is extensively utilized 11 , 24 , 34 . However, this highly invasive procedure may lead to significant injuries and uncertainties during experimentation (e.g., poor healing of the large wounds, difficulty in removing the catheter).…”

Section: Discussionmentioning

confidence: 99%

“…Those large animals are used in complex and discontinuous blood exchange methods, resulting in high experimental costs and time, leading to high failure rates 12 , 22 . Recent studies have explored alternative methods for blood exchange in animals, such as transfusion of small animals through intermittent infusion by the use of microcirculation electronic pumps 10 , 24 . Although these research methods 10 , 24 have reduced experimental costs, simplified procedures, and increased experimental control compared to previous study 16 , they 10 , 24 often require pre-implanted jugular veins, which can lead to serious surgical injuries.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have explored alternative methods for blood exchange in animals, such as transfusion of small animals through intermittent infusion by the use of microcirculation electronic pumps 10 , 24 . Although these research methods 10 , 24 have reduced experimental costs, simplified procedures, and increased experimental control compared to previous study 16 , they 10 , 24 often require pre-implanted jugular veins, which can lead to serious surgical injuries. And those methods has limitations include intermittent plasma exchange 10 , a small exchange volume per time 25 .…”

Section: Introductionmentioning

confidence: 99%

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Continuous blood exchange in rats as a novel approach for experimental investigation

Pei,

Wang,

Zhang

et al. 2024

Sci Rep

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Blood exchange therapy, specifically Whole blood exchange (WBE), is increasingly being utilized in clinical settings to effectively treat a range of diseases. Consequently, there is an urgent requirement to establish convenient and clinically applicable animal models that can facilitate the exploration of blood exchange therapy mechanisms. Our study conducted continuous WBE in rats through femoral and tail vein catheterization using dual-directional syringe pumps. To demonstrate the applicability of continuous WBE, drug-induced hemolytic anemia (DIHA) was induced through phenylhydrazine hydrochloride (PHZ) injection. Notability, the rats of DIHA + WBE group all survived and recovered within the subsequent period. After the implementation of continuous WBE therapy day (Day 1), the DIHA + WBE group exhibited a statistically significant increase in red blood cells (RBC) (P = 0.0343) and hemoglobin (HGB) levels (P = 0.0090) compared to DIHA group. The rats in the DIHA + WBE group exhibited a faster recovery rate compared to the DIHA group, indicating the successful establishment of a continuous blood exchange protocol. This experimental approach demonstrates not just promising efficacy in the treatment of DIHA and offers a valuable tool for investigating the underlying mechanisms of blood exchange. Furthermore, it has a great potential to the advancement of biomedical research such as drug delivery exploration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Continuous blood exchange in rats as a novel approach for experimental investigation

Pei,

Wang,

Zhang

et al. 2024

Sci Rep

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“…Since humoral factors can enter the brain at the BBB and BCsfB through receptor-mediated transcytosis and micropinocytosis, in recent years, in vivo models, for example, heterochronic parabiosis (HB), heterochronic blood exchange (HBE) [ 4 , 5 , 6 , 7 , 8 ], and infusions of small volumes of plasma or serum, have been used to uncover and better understand the role of those factors in aging and age-related diseases [ 9 , 10 , 11 , 12 ]. Recently, using infusions of small volumes of serum, we evaluated the contribution of circulating EVs to the beneficial effect of young serum on aged muscle stem cell (MuSC) function and the skeletal muscle regenerative cascade [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Extracellular Vesicles in Young Serum Contribute to the Restoration of Age-Related Brain Transcriptomes and Cognition in Old Mice

Fitz

Sahu

Lee

et al. 2023

IJMS

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We have previously demonstrated that circulating extracellular vesicles (EVs) are essential to the beneficial effect of young serum on the skeletal muscle regenerative cascade. Here, we show that infusions of young serum significantly improve age-associated memory deficits, and that these effects are abolished after serum depletion of EVs. RNA-seq analysis of the choroid plexus demonstrates EV-mediated effects on genes involved in barrier function and trans-barrier transport. Comparing the differentially expressed genes to recently published chronological aging clock genes reveals a reversal of transcriptomic aging in the choroid plexus. Following young serum treatment, the hippocampal transcriptome demonstrates significant upregulation of the anti-aging gene Klotho, along with an abrogated effect after EV depletion. Transcriptomic profiling of Klotho knockout and heterozygous mice shows the downregulation of genes associated with transport, exocytosis, and lipid transport, while upregulated genes are associated with activated microglia. The results of our study indicate the significance of EVs as vehicles to deliver signals from the periphery to the brain and the importance of Klotho in maintaining brain homeostasis.

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“…Due to their similar anatomical and physiological characteristics to humans, large animals such as lambs, dogs, and pigs were used in blood exchange models [10,12,20,21] . Those large animals are used in complex and discontinuous blood exchange methods, resulting in high experimental costs and time, leading to high failure rates [10,20] .Recent studies have explored alternative methods for blood exchange in animals, such as transfusion of small animals through intermittent infusion by the use of microcirculation electronic pumps [8,22] . Although these research methods [8,22] have reduced experimental costs, simplified procedures, and increased experimental control compared to previous study [14] , they [8,22] often require pre-implanted jugular veins, which can lead to serious surgical injuries.…”

Section: Introductionmentioning

confidence: 99%

Continuous Blood Exchange in Rats: A Protocol for Experimental Investigation

Pei,

Wang,

Zhang

et al. 2023

Preprint

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Blood exchange therapy, specifically whole blood exchange (WBE), is increasingly being utilized in clinical settings to effectively treat a range of diseases. Consequently, there is an urgent requirement to establish convenient and clinically applicable animal models that can facilitate the exploration of blood exchange therapy mechanisms. Our study conducted continuous whole blood exchange (WBE) in rats through femoral and tail vein catheterization using dual-directional syringe pumps. To demonstrate the applicability of continuous whole blood exchange, drug-induced hemolytic anemia (DIHA) was induced through phenylhydrazine hydrochloride injection. Notability, the rats of WBE treatment group survived all and recovered within the subsequent period. After the implementation of continuous whole blood exchange therapy day (Day 1), the treatment group exhibited a statistically significant increase in red blood cells (P = 0.0343) and hemoglobin levels (P = 0.0090). The rats in the WBE treatment group exhibited a faster recovery rate compared to the model group, indicating the successful establishment of a continuous blood exchange protocol. This experimental approach demonstrates not just promising efficacy in the treatment of DIHA and offers a valuable tool for investigating the underlying mechanisms of blood exchange. Furthermore, it has a great potential to the advancement of biomedical research such as drug delivery exploration.

show abstract

Small-animal blood exchange is an emerging approach for systemic aging research

Cited by 9 publications

References 41 publications

Continuous blood exchange in rats as a novel approach for experimental investigation

Continuous blood exchange in rats as a novel approach for experimental investigation

Extracellular Vesicles in Young Serum Contribute to the Restoration of Age-Related Brain Transcriptomes and Cognition in Old Mice

Continuous Blood Exchange in Rats: A Protocol for Experimental Investigation

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