Small Animal Computed Tomography Imaging

Bartling, Sönke; Stiller, Wolfram; Semmler, Willi; Kießling, Fabian

doi:10.2174/157340507779940327

Cited by 65 publications

(50 citation statements)

References 68 publications

(143 reference statements)

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“…Using these settings, we demonstrated that the resolution achieved by VCT was sufficient to identify alterations of the lung texture and the larger airways in 3-day-old neonatal mice with a body weight of ,2-3 g. The maximum cumulative dose of 10 subsequent VCT scans was ,230 mGy [23], which was well tolerated by mice in our study and previous studies [36,37], thus allowing noninvasive longitudinal monitoring of lung disease in mice from neonatal to adult ages.…”

Section: Discussionmentioning

confidence: 54%

In vivomonitoring of cystic fibrosis-like lung disease in mice by volumetric computed tomography

Wielpütz¹,

Eichinger²,

Zhou³

et al. 2011

Eur Respir J

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The onset and spontaneous development of cystic fibrosis (CF) lung disease remain poorly understood. In the present study, we used volumetric computed tomography (VCT) as a new method for longitudinal in vivo monitoring of early lesions and disease progression in CF-like lung disease in b-epithelial Na + channel (ENaC)-transgenic (TG) mice.Using a VCT scanner prototype (80 kV, 50 mA?s, scan time 19 s and spatial resolution 200 mm), bENaC-TG mice and wild-type (WT) littermates were examined longitudinally at 10 time-points from neonatal to adult ages, and VCT images were assessed by qualitative and quantitative morphological parameters.We demonstrate that VCT detected early-onset airway mucus obstruction, diffuse infiltrates, atelectasis and air trapping as characteristic abnormalities in bENaC-TG mice. Furthermore, we show that early tracheal mucus obstruction predicted mortality in bENaC-TG mice and that the density of lung parenchyma was significantly reduced at all time-points in bENaC-TG compared with WT mice (median¡SEM -558¡8 HU in WT versus -686¡16 HU in bENaC-TG at 6 weeks of age; p,0.005).Our study demonstrates that VCT is a sensitive, noninvasive technique for early detection and longitudinal monitoring of morphological abnormalities of CF-like lung disease in mice, and may thus provide a useful tool for pre-clinical in vivo evaluation of novel treatment strategies for CF.

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Section: Discussionmentioning

confidence: 54%

In vivomonitoring of cystic fibrosis-like lung disease in mice by volumetric computed tomography

Wielpütz¹,

Eichinger²,

Zhou³

et al. 2011

Eur Respir J

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“…(AU = arbitrary unit) vessels. For this purpose, 2.5-ml Fenestra-VC (ART Advanced Research Technologies, Saint-Laurent, CA)-a blood pool contrast agent with 50 mg iodine/ml-was injected into the tail vein of the rats, 5 min prior to the data acquisition [1,17]. The mice were given 0.5 ml of the same contrast agent.…”

Section: Animals Contrast Mediamentioning

confidence: 99%

Intrinsic respiratory gating in small-animal CT

et al. 2008

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Gating in small-animal CT imaging can compensate artefacts caused by physiological motion during scanning. However, all published gating approaches for small animals rely on additional hardware to derive the gating signals. In contrast, in this study a novel method of intrinsic respiratory gating of rodents was developed and tested for mice (n=5), rats (n=5) and rabbits (n=2) in a flat-panel cone-beam CT system. In a consensus read image quality was compared with that of non-gated and retrospective extrinsically gated scans performed using a pneumatic cushion. In comparison to non-gated images, image quality improved significantly using intrinsic and extrinsic gating. Delineation of diaphragm and lung structure improved in all animals. Image quality of intrinsically gated CT was judged to be equivalent to extrinsically gated ones. Additionally 4D datasets were calculated using both gating methods. Values for expiratory, inspiratory and tidal lung volumes determined with the two gating methods were comparable and correlated well with values known from the literature. We could show that intrinsic respiratory gating in rodents makes additional gating hardware and preparatory efforts superfluous. This method improves image quality and allows derivation of functional data. Therefore it bears the potential to find wide applications in small-animal CT imaging.

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“…It has been reported that doses of more than 5 Gy could be considered as lethal in mice [20,21]. It is recognized that exposure to repeated radiations during follow-up studies can have biological effects on the animal models and thus can affect the experimental results.…”

Section: Discussionmentioning

confidence: 99%

Dose Calculation in a Mouse Lung Tumor and in Secondary Organs During Radiotherapy Treatment: A Monte Carlo Study

Hamdi

Mimi

Bentourkia

2015

Bioinformatics and Biomedical Engineering

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Abstract. Radiotherapy in cancer treatment always affects surrounding tissues and even deposits doses in distant tissues not traversed by the radiation beams. In the present work, we report energy transfer and absorbed dose in a target tumor and in other distant organs in a digital mouse by Monte Carlo simulations. We simulated a selection of X-rays beams with seven energies, 50, 100, 150, 200, 250, 350 and 450 keV each oriented in seven irregularly incremented angles, and we computed the dose and the energy deposit as a function of photon interaction types. The results show that the absorbed dose increased with increasing energy even in the secondary organs not receiving the radiation beam, and that the lowest dose was obtained with 100 keV beam. The spinal cord, of comparable size to the tumor and excluding the spinal bones, which was not directly irradiated by the beams, received a dose representing in average 1% of that of the tumor, while the spinal bone received doses of 6.6 and 0.12 times those in the tumor at 50 and 450 keV, respectively. Such Monte Carlo simulations could be necessary to select the appropriate beam energy and beam angles to efficiently treat the tumor and to moderately reduce the impact of the radiations in the other organs.

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Small Animal Computed Tomography Imaging

Cited by 65 publications

References 68 publications

In vivomonitoring of cystic fibrosis-like lung disease in mice by volumetric computed tomography

In vivomonitoring of cystic fibrosis-like lung disease in mice by volumetric computed tomography

Intrinsic respiratory gating in small-animal CT

Dose Calculation in a Mouse Lung Tumor and in Secondary Organs During Radiotherapy Treatment: A Monte Carlo Study

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