SMALL AUXIN UP RNA62/75 Are Required for the Translation of Transcripts Essential for Pollen Tube Growth

He, Siou-Luan; Hsieh, Hsun-Ping; Jauh, Guang‐Yuh

doi:10.1104/pp.18.00257

Cited by 25 publications

(19 citation statements)

References 68 publications

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“…Indeed, heat stress inhibits elongation of the pollen tube into the ovary by impairing auxin homeostasis in pollinated pistils, which results in sterile spikelets [33]. However, in this study, auxin might not be the determining factor in this process, although this hormone plays a key role in pollen tube elongation in plants [34–37]. The highest auxin level was found in plants treated with sucrose at 5 DAF, while no significant difference was found between the control and sucrose plus ABA treatment (Fig.…”

Section: Discussionmentioning

confidence: 91%

Abscisic acid synergizes with sucrose to enhance grain yield and quality of rice by improving the source-sink relationship

Chen

Islam

et al. 2019

BMC Plant Biol

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BackgroundAbscisic acid (ABA) and sucrose act as molecular signals in response to abiotic stress. However, how their synergy regulates the source-sink relationship has rarely been studied. This study aimed to reveal the mechanism underlying the synergy between ABA and sucrose on assimilates allocation to improve grain yield and quality of rice. The early indica rice cultivar Zhefu802 was selected and planted in an artificial climate chamber at 32/24 °C (day/night) under natural sunlight conditions. Sucrose and ABA were exogenously sprayed (either alone or in combination) onto rice plants at flowering and 10 days after flowering.ResultsABA plus sucrose significantly improved both the grain yield and quality of rice, which was mainly a result of the higher proportion of dry matter accumulation and non-structural carbohydrates in panicles. These results were mainly ascribed to the large improvement in sucrose transport in the sheath-stems in response to the ABA plus sucrose treatment. In this process, ABA plus sucrose significantly enhanced the contents of starch, gibberellic acids, and zeatin ribosides as well as the activities and gene expression of enzymes involved in starch synthesis in grains. Additionally, remarkable increases in trehalose content and expression levels of trehalose-6-phosphate synthase1, trehalose-6-phosphate phosphatase7, and sucrose non-fermenting related protein kinase 1A were also found in grains treated with ABA plus sucrose.ConclusionThe synergy between ABA and sucrose increased grain yield and quality by improving the source-sink relationship through sucrose and trehalose metabolism in grains.

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Section: Discussionmentioning

confidence: 91%

Abscisic acid synergizes with sucrose to enhance grain yield and quality of rice by improving the source-sink relationship

Chen

Islam

et al. 2019

BMC Plant Biol

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“…SAUR19 directly interacts with D-clade type 2C protein phosphatases (PP2C-D) to promote cell expansion by enhancing activity of plasma membrane (PM) H + -ATPase (Spartz et al, 2014). Some other Arabidopsis SAUR genes are implicated in plant growth and development regulation (Ren & Gray, 2015;He et al, 2018). Although the majority of SAUR genes that have been studied are positive regulators of plant growth (Chae et al, 2012;Spartz et al, 2012;Kong et al, 2013;Li et al, 2015), negative regulation has also been suggested for a few SAURs (Hou et al, 2013;Markakis et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Natural variations of growth thermo‐responsiveness determined by SAUR26/27/28 proteins in Arabidopsis thaliana

et al. 2019

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Summary How diversity in growth thermo‐responsiveness is generated for local adaptation is a long‐standing biological question. We investigated molecular genetic basis of natural variations in thermo‐responsiveness of plant architecture in Arabidopsis thaliana. We measured the extent of rosette architecture at 22°C and 28°C in a set of 69 natural accessions and determined their thermo‐responsiveness of plant architecture. A genome‐wide association study was performed to identify major loci for variations in thermo‐responsiveness. The SAUR26 subfamily, a new subfamily of SAUR genes, was identified as a major locus for the thermo‐responsive architecture variations. The expression of SAUR26/27/28 is modulated by temperature and PIF4. Extensive natural polymorphisms in these genes affect their RNA expression levels and protein activities and influence the thermo‐responsiveness of plant architecture. In addition, the SAUR26 subfamily genes exhibit a high variation frequency and their variations are associated with the local temperature climate. This study reveals that the SAUR26 subfamily is a key variation for thermo‐responsive architecture and suggests a preference for generating diversity for local adaptation through signaling connectors.

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“…Auxin and brassinosteroid (BR) are two major classes of hormone, which are considered as master regulators in different plant development processes and response to various biotic and abiotic stresses [ 43 , 44 ]. In addition, the role of auxin and BR and their binding proteins is well-known in anther development, pollen tube growth, and subsequent fertilization [ 45 ]. There was a significant difference in DEGs related to auxins, BR and abscisic acid metabolism, which corroborates with previous findings, stating that plants employ the iterative trade-off to modulate differential organ growth upon the elicitation of immune response [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated Proteo-Transcriptomic Analyses Reveal Insights into Regulation of Pollen Development Stages and Dynamics of Cellular Response to Apple Fruit Crinkle Viroid (AFCVd)-Infection in Nicotiana tabacum

Shrestha

Mishra

Matoušek

et al. 2020

IJMS

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Tobacco (Nicotiana tabacum) pollen is a well-suited model for studying many fundamental biological processes owing to its well-defined and distinct development stages. It is also one of the major agents involved in the transmission of infectious viroids, which is the primary mechanism of viroid pathogenicity in plants. However, some viroids are non-transmissible and may be possibly degraded or eliminated during the gradual process of pollen development maturation. The molecular details behind the response of developing pollen against the apple fruit crinkle viroid (AFCVd) infection and viroid eradication is largely unknown. In this study, we performed an integrative analysis of the transcriptome and proteome profiles to disentangle the molecular cascade of events governing the three pollen development stages: early bicellular pollen (stage 3, S3), late bicellular pollen (stage 5, S5), and 6 h-pollen tube (PT6). The integrated analysis delivered the molecular portraits of the developing pollen against AFCVd infection, including mechanistic insights into the viroid eradication during the last steps of pollen development. The isobaric tags for label-free relative quantification (iTRAQ) with digital gene expression (DGE) experiments led us to reliably identify subsets of 5321, 5286, and 6923 proteins and 64,033, 60,597, and 46,640 expressed genes in S3, S5, and PT6, respectively. In these subsets, 2234, 2108 proteins and 9207 and 14,065 mRNAs were differentially expressed in pairwise comparisons of three stages S5 vs. S3 and PT6 vs. S5 of control pollen in tobacco. Correlation analysis between the abundance of differentially expressed mRNAs (DEGs) and differentially expressed proteins (DEPs) in pairwise comparisons of three stages of pollen revealed numerous discordant changes in mRNA/protein pairs. Only a modest correlation was observed, indicative of divergent transcription, and its regulation and importance of post-transcriptional events in the determination of the fate of early and late pollen development in tobacco. The functional and enrichment analysis of correlated DEGs/DEPs revealed the activation in pathways involved in carbohydrate metabolism, amino acid metabolism, lipid metabolism, and cofactor as well as vitamin metabolism, which points to the importance of these metabolic pathways in pollen development. Furthermore, the detailed picture of AFCVd-infected correlated DEGs/DEPs was obtained in pairwise comparisons of three stages of infected pollen. The AFCVd infection caused the modulation of several genes involved in protein degradation, nuclear transport, phytohormone signaling, defense response, and phosphorylation. Intriguingly, we also identified several factors including, DNA-dependent RNA-polymerase, ribosomal protein, Argonaute (AGO) proteins, nucleotide binding proteins, and RNA exonucleases, which may plausibly involve in viroid stabilization and eradication during the last steps of pollen development. The present study provides essential insights into the transcriptional and translational dynamics of tobacco pollen, which further strengthens our understanding of plant-viroid interactions and support for future mechanistic studies directed at delineating the functional role of candidate factors involved in viroid elimination.

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SMALL AUXIN UP RNA62/75 Are Required for the Translation of Transcripts Essential for Pollen Tube Growth

Cited by 25 publications

References 68 publications

Abscisic acid synergizes with sucrose to enhance grain yield and quality of rice by improving the source-sink relationship

Abscisic acid synergizes with sucrose to enhance grain yield and quality of rice by improving the source-sink relationship

Natural variations of growth thermo‐responsiveness determined by SAUR26/27/28 proteins in Arabidopsis thaliana

Integrated Proteo-Transcriptomic Analyses Reveal Insights into Regulation of Pollen Development Stages and Dynamics of Cellular Response to Apple Fruit Crinkle Viroid (AFCVd)-Infection in Nicotiana tabacum

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