Small conductance calcium-activated potassium channels and nitric oxide/cGMP pathway mediate cardioprotective effects of Croton urucurana Baill. In hypertensive rats

Lopes, Katiana Simões; Marques, Aline Aparecida Macedo; Moreno, Karyne Garcia Tafarelo; Lorençone, Bethânia Rosa; Leite, Patrícia Regina Terço; Silva, Gabriela Pereira da; Santos, Ariany Carvalho dos; Souza, Roosevelt Isaías Carvalho; Gasparotto, Francielly Mourão; Cassemiro, Nadla Soares; Lourenço, Emerson Luiz Botelho; Klider, Lislaine Maria; Budel, Jane Manfron; Silva, Denise Brentan

doi:10.1016/j.jep.2022.115255

Cited by 7 publications

(4 citation statements)

References 42 publications

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“…The presence or absence of crystals and their type and chemical composition support plant taxonomy. Crystals have been described in some studies as calcium oxalate by using EDS [29][30][31][32][33].…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Description of the Anatomy and Histochemistry of Psychotria capillacea (Müll. Arg.) Standl. and an Investigation into Its Anti-Inflammatory Effects in Mice and Role in Scopolamine-Induced Memory Impairment

Formagio,

Vilegas,

Kassuya

et al. 2024

Pharmaceuticals

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Species of the genus Psychotria are used in popular medicine for pain, inflammatory symptoms, and mental disorders. Psychotria capillacea (Müll. Arg.) Standl. (Rubiaceae) is commonly known as coffee and some scientific studies have demonstrated its therapeutic potential. The goal of this study was to investigate the anti-inflammatory and neuroprotective effects, and acetylcholinesterase (AChE) inhibitory activity of a methanolic extract obtained from leaves of P. capillacea (MEPC), as well as the micromorphology and histochemistry of the leaves and stems of this plant. In addition, the MEPC was analyzed by UHPLC-MS/MS and the alkaloidal fraction (AF) obtained from the MEPC was tested in a mouse model of inflammation. MEPC contained three indole alkaloids, one sesquiterpene (megastigmane-type) and two terpene lactones. MEPC (3, 30 and 100 mg/kg) and AF (3 and 30 mg/kg) were evaluated in inflammation models and significantly inhibited edema at 2 h and 4 h, mechanical hyperalgesia after 4 h and the response to cold 3 h and 4 h after carrageenan injection. Scopolamine significantly increased the escape latency, and reduced the swimming time and number of crossings in the target quadrant and distance, while MEPC (3, 30 and 100 mg/kg), due to its neuroprotective actions, reversed these effects. AChE activity was significantly decreased in the cerebral cortex (52 ± 3%) and hippocampus (60 ± 3%), after MEPC administration. Moreover, micromorphological and histochemical information was presented, to aid in species identification and quality control of P. capillacea. The results of this study demonstrated that P. capillacea is an anti-inflammatory and antihyperalgesic agent that can treat acute disease and enhance memory functions in mouse models.

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Section: Discussionmentioning

confidence: 99%

A Comprehensive Description of the Anatomy and Histochemistry of Psychotria capillacea (Müll. Arg.) Standl. and an Investigation into Its Anti-Inflammatory Effects in Mice and Role in Scopolamine-Induced Memory Impairment

Formagio,

Vilegas,

Kassuya

et al. 2024

Pharmaceuticals

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“…The SK channels play an important role in the cardioprotective effects of prolonged administration of an extract from leaves of Croton urucurana Baill. ( Euphorbiaceae ), which is popularly known as ‘sangue de dragão’, according to the spontaneously hypertensive rat model [ 149 ]. Flavonoids (including rutin, isoquercetin, kaempferol, vitexin) are the key bioactive substances in this extract [ 150 ].…”

Section: Calcium-activated Channels (Kca)mentioning

confidence: 99%

Flavonoids as Modulators of Potassium Channels

Richter

Trybek

Delfino

et al. 2023

IJMS

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Potassium channels are widely distributed integral proteins responsible for the effective and selective transport of K+ ions through the biological membranes. According to the existing structural and mechanistic differences, they are divided into several groups. All of them are considered important molecular drug targets due to their physiological roles, including the regulation of membrane potential or cell signaling. One of the recent trends in molecular pharmacology is the evaluation of the therapeutic potential of natural compounds and their derivatives, which can exhibit high specificity and effectiveness. Among the pharmaceuticals of plant origin, which are potassium channel modulators, flavonoids appear as a powerful group of biologically active substances. It is caused by their well-documented anti-oxidative, anti-inflammatory, anti-mutagenic, anti-carcinogenic, and antidiabetic effects on human health. Here, we focus on presenting the current state of knowledge about the possibilities of modulation of particular types of potassium channels by different flavonoids. Additionally, the biological meaning of the flavonoid-mediated changes in the activity of K+ channels will be outlined. Finally, novel promising directions for further research in this area will be proposed.

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“…All procedures were previously approved by the Ethics Committee in Animal Experimentation from the UFGD (protocol no. 31/2019-1) [10,11] and were following the Brazilian Legal Framework on Scientifc Animals Use.…”

Section: Pharmacological Investigationsmentioning

confidence: 99%

Cardioprotective Effects of Solidago microglossa DC. in Nicotine‐Treated Hypertensive Rats

Lopes,

Marques,

Moreno

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Solidago microglossa DC. (Asteraceae), “arnica brasileira,” is a Brazilian species popularly used to treat hypertension or renal ailments. This study investigated the cardioprotective effects of standardized S. microglossa extract (EESM) in nicotine-treated spontaneously hypertensive rats (SHRs). Moreover, the molecular mechanisms involved in the cardiovascular effects were also investigated. The acute toxicity was evaluated in female Wistar rats. Afterwards, six-month-old male spontaneously hypertensive rats received the EESM (14, 28, and 56 mg/kg), hydrochlorothiazide (25 mg/kg), and vehicle (filtered water; 0.1 mL/100 g) once daily for 28 days. All treatments were associated with 1.8 mg/kg of nicotine. At the end of the experimental period, the renal function, electrocardiographic profile, blood pressure, ventricular function, biochemical parameter, and mesenteric vascular bed reactivity were evaluated. Relative organ weights and cardiac morphometry were also investigated. Nicotine treatment in 6-month-old SHRs induced a significant reduction in renal function, with reduced urinary volume and lower renal elimination of sodium and creatinine. In addition, serum markers of the redox state and blood pressure levels remained significantly elevated, contributing to changes in vascular reactivity and left ventricular hypertrophy associated with reduced ventricular function. After 28 days of treatment, we found that the highest dose of EESM could mitigate all renal and cardiovascular changes developed by the nicotine-treated hypertensive rats. This study presented EESM as a possible cardioprotective drug that prevents cardiovascular dysfunctions in nicotine-treated hypertensive rats. Our data suggest EESM as a potential adjuvant therapy when cardioprotective effects are required.

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Small conductance calcium-activated potassium channels and nitric oxide/cGMP pathway mediate cardioprotective effects of Croton urucurana Baill. In hypertensive rats

Cited by 7 publications

References 42 publications

A Comprehensive Description of the Anatomy and Histochemistry of Psychotria capillacea (Müll. Arg.) Standl. and an Investigation into Its Anti-Inflammatory Effects in Mice and Role in Scopolamine-Induced Memory Impairment

A Comprehensive Description of the Anatomy and Histochemistry of Psychotria capillacea (Müll. Arg.) Standl. and an Investigation into Its Anti-Inflammatory Effects in Mice and Role in Scopolamine-Induced Memory Impairment

Flavonoids as Modulators of Potassium Channels

Cardioprotective Effects of Solidago microglossa DC. in Nicotine‐Treated Hypertensive Rats

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