Small DNA Densonucleosis Virus (DNV)

Kurstak, Edouard

doi:10.1016/s0065-3527(08)60751-4

Cited by 47 publications

(13 citation statements)

References 40 publications

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“…In each of these studies with viruses known to replicate their DNA in the nucleus, autoradiographic grains representing viral DNA were confined to the nuclei of infected cells. Similarly, intranuclear inclusions in cells infected with parvoviruses (Kurstak, 1972), papovaviruses (Oxman, 1978), adenoviruses (Ledinko, 1978) and herpesviruses (Crouse et al, 1950) are usually Feulgen-positive, whereas those in HBV-infected hepatocytes are Feulgen-negative (Huang et al, 1972;Bianchi & Gudat, 1976). Thus, several lines of consistent evidence relating to the cellular localization of viral DNA provide a contrast between HBV on one hand, and other DNA viruses known to replicate their DNA in the cell nucleus on the other hand.…”

Section: Discussionmentioning

confidence: 99%

Patterns of Single- and Double-stranded Hepatitis B Virus DNA and Viral Antigen Accumulation in Infected Liver Cells

Gowans¹,

Burrell²,

Jilbert³

et al. 1983

Journal of General Virology

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SUMMARYLiver sections from five patients with persistent hepatitis B virus (HBV) infection and active cirrhosis were shown to contain intracellular HBV DNA by in situ hybridization using cloned 3H-labelled HBV DNA probes. Two classes of infected cells, with different distributions throughout the liver, were distinguished: (i) cells containing a low copy number of double-stranded HBV nucleotide sequences, confined to the cell nucleus and thought to represent HBV DNA, and (ii) cells containing large amounts (estimated to be greater than 10 or 15 genome copies per cell) of HBV DNA, much of it in a single-stranded form and largely confined to the cell cytoplasm; these single-stranded regions represented widely separated regions of the HBV genome, in contrast to the structure of the DNA in mature virions. It is likely that these latter cells may be supporting viral DNA synthesis. Cells with large amounts of cytoplasmic HBV DNA invariably contained hepatitis B surface antigen (HBsAg) and in addition contained either no detectable hepatitis B core antigen (HBcAg), or cytoplasmic HBcAg or nuclear HBcAg in that order of frequency. Cytoplasmic HBcAg was highly predictive of the presence of large amounts of cytoplasmic HBV DNA in the same cell, while either nuclear HBcAg, or cytoplasmic HBsAg, were often seen both in cells with and without such levels of DNA. These patterns, relating HBV DNA and antigen content in naturally occurring asynchronous infection in a heterogeneous cell population, should provide a background to further studies of the virus replication cycle with a defined experimental system, when such a system becomes available. INTRODUCTIONPersistent infection with hepatitis B virus (HBV) in man is associated with different histological types of chronic liver disease in different patients, that reflect varying levels of active hepatocyte damage and inflammation, together with varying degrees of disturbance of liver architecture.In general, (i) healthy patients with minimal hepatocyte damage tend to have significant expression of hepatitis B surface antigen (HBsAg) within hepatocyte cytoplasm, (ii) immunosuppressed patients with minimal liver damage show abundant intranuclear hepatitis B core antigen (HBcAg) together with cytoplasmic membrane expression of HBsAg and (iii) patients with active liver damage show 'spotty' expression of both HBcAg and HBsAg (Gudat et al., 1975;Gerber & Thung, 1979). Three additional markers have been postulated to identify those patients with a higher general level of HBV replication within infected tissues. These are (i) the presence of detectable HBV DNA (or HBV virions) in a patient's serum (Werner et al., 1977;Bonino et al., 1981 ; Brechot et al., 1981 b), (ii) the presence of non-integrated 'free' HBV DNA in DNA extracts from infected liver (Brechot et al., 1981 a, b) and (iii) the presence of hepatitis B e antigen (HBeAg), rather than hepatitis B e antibody (anti-HBe), in a patient's serum. In different patients, the above markers of virus 'replication' tend to coexist, and...

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Section: Discussionmentioning

confidence: 99%

Patterns of Single- and Double-stranded Hepatitis B Virus DNA and Viral Antigen Accumulation in Infected Liver Cells

Gowans¹,

Burrell²,

Jilbert³

et al. 1983

Journal of General Virology

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“…The results presented above indicate that the newly isolated "cockroach virus" has many characteristics related to those of the densonucleosis virus, a parvovirus isolated from the greater wax moth, Galleria mellonella (Linnaeus) (2). However, for the determination of its definitive position in the family Parvoviridae (1, 6), further biochemical, immunological, and electron microscopic studies are required.…”

mentioning

confidence: 97%

A New Virus Isolated from the Cockroach, Periplaneta fuliginosa (Serville)

Suto

Kawamoto

Kumada

1979

Microbiology and Immunology

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“…This means that most of the virions (about 90%) had encapsidated strands of the same polarity, by convention the minus strand [10], and only few of them had encapsidated a strand of complementary (+) polarity. In this respect, AaPV differs from most insect parvoviruses and adeno-associated viruses (AAV) so far isolated which encapsidate strands of both polarities in equal proportion to form linear double-stranded DNA molecules under high ionic strength conditions of extraction [7,10,13,24,25,28,30,31,35,39]. This in turn suggests that the mechanism of replication and (or) encapsidation of AaPV genome is similar to that of autonomous parvoviruses which encapsidate essentially minus strands (for review see [36]).…”

Section: Discussionmentioning

confidence: 99%

Structure, restriction map and infectivity of the genomic and replicative forms ofAaPV DNA

Boublik

Jousset

Bergoin

1994

Archives of Virology

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We have characterized the genomic and replicative form (RF) DNA of the Aedes albopictus Parvovirus (AaPV), a virus isolated from a chronically infected C6/36 clone of Aedes albopictus cell line [22]. The genome of AaPV virions is a single-stranded linear DNA molecule approximately 4.2 kb in length, essentially (about 90%) encapsidated as minus strand. A restriction map of the RF DNA isolated from infected C6/36 cells was established. Among the 23 restriction enzymes tested, 14 cleaved the AaPV RF DNA and 30 restriction sites were mapped and oriented with respect to the viral genomic DNA. Both viral and RF DNAs were found infectious when transfected to virus-free C6/36 cells. The asymmetrical encapsidation of the viral genome is a property common to most vertebrate autonomous parvoviruses but rather unusual among densoviruses. Both by its small size, the asymmetrical mode of encapsidation and the restriction map, the AaPV genome resembles that of the Aedes Densonucleosis virus [1].

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Small DNA Densonucleosis Virus (DNV)

Cited by 47 publications

References 40 publications

Patterns of Single- and Double-stranded Hepatitis B Virus DNA and Viral Antigen Accumulation in Infected Liver Cells

Patterns of Single- and Double-stranded Hepatitis B Virus DNA and Viral Antigen Accumulation in Infected Liver Cells

A New Virus Isolated from the Cockroach, Periplaneta fuliginosa (Serville)

Structure, restriction map and infectivity of the genomic and replicative forms ofAaPV DNA

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