Small form factor flow virometer for SARS-CoV-2

Hussain, Rubaiya; Ongaro, Alfredo Edoardo; Concepción, Maria Luisa Rodrı́guez de la; Wajs, Ewelina; Riveira-Muñoz, Eva; Ballana, Ester; Blanco, Julià; Toledo, Renata Ferraz de; Chamorro, Anna; Massanella, Marta; Mateu, Lourdes; Grau, Eulàlia; Clotet, Bonaventura; Carrillo, Jorge; Pruneri, Valerio

doi:10.1364/boe.450212

Cited by 3 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cellular properties of platelets can be studied on-chip by integrating the existing microdevice with a detection zone as shown in figure 5. The detection zone may consist of flow-virometry reader (FVR), direct serological biosensor assay using proprietary technology based on plasmonic, and antibody or molecular or immunoassay-based detection units [33,34].…”

Section: Microdevice For On-chip Study Of Plateletsmentioning

confidence: 99%

“…For the detection of COVID-19 infection the side daughter branches of the existing microdevice can be slightly modified for the isolation of pure plasma from whole blood sample. Later, the microdevice can be integrated with ELISA (enzyme-linked immunoabsorbent assay), lateral flow assay, flow-virometry reader (FVR), direct serological biosensor assay [33,34] as shown in figure 6. This arrangement is not limited to detection of COVID-19, other tests can also be performed by replacing the antigens of interest coated at the plasma outlet.…”

Section: Separation Of Plasma Sample For On-chip Detection Of Covid-1...mentioning

confidence: 99%

See 1 more Smart Citation

A lab-on-chip solution for the detection and suppression of COVID-19 severity

Laxmi

Joshi

Agrawal³

2023

Biomed. Phys. Eng. Express

View full text Add to dashboard Cite

At present, the conventional method for detecting COVID-19 infection is reverse transcription-polymerase chain reaction (RT-PCR) performed on nasopharyngeal and pharyngeal swabs. In addition, other detection techniques such as isothermal nucleic acid amplification, enzyme-linked immunosorbent assay (ELISA) have also been proposed and are being used. However, these techniques are limited to central hospitals and pathologies. Point-of-care solutions may play an important role in rapid and timely detection by an individual at their doorstep. In addition, the disease adversely affects the functioning of various constituents of human blood. For example, it reduces the platelets count, increases production of proinflammatory cytokine which results in cytokine storm, and increases level of various plasma proteins with the severity of the disease. Therefore, monitoring and transfusion of blood plasma and platelets may play an essential role in detection and suppression of severity of COVID-19 infection. In this regard, we propose evolution of our existing microdevice for the detection and suppression of severity of COVID-19 infection. We propose modification of our microdevice in the following ways (i) as a platelet-rich plasma separation unit, (ii) as an on-chip device to study inter-cellular properties of platelets, and (iii) for on-chip detection of infection by separating plasma. The integration of these microdevices with lateral flow assays, flow-virometry reader (FVR), direct serological biosensor assay along with proprietary technology based on plasmonic, place these microdevices in an advantageous position. Moreover, coupling of the microdevices with machine leaning application for rapid detection of the severity of COVID-19 and platelets related disorders makes these microdevices as a complete unit for point-of-care application.

show abstract

Section: Microdevice For On-chip Study Of Plateletsmentioning

confidence: 99%

Section: Separation Of Plasma Sample For On-chip Detection Of Covid-1...mentioning

confidence: 99%

A lab-on-chip solution for the detection and suppression of COVID-19 severity

Laxmi

Joshi

Agrawal³

2023

Biomed. Phys. Eng. Express

View full text Add to dashboard Cite

show abstract

Saliva Analysis Based on Microfluidics: Focusing the Wide Spectrum of Target Analyte

Liu,

Zhou,

et al. 2023

Critical Reviews in Analytical Chemistry

View full text Add to dashboard Cite

Point-of-care real-time DNA detection device for SARS-CoV-2 from clinical samples

et al. 2023

View full text Add to dashboard Cite

Since the global pandemic of SARS-CoV-2, people’s health and the economic support of their countries have been seriously affected. It was necessary to develop a low-cost and faster diagnostic tool that allows the evaluation of symptomatic patients. Point-of-care testing and point-of-need testing systems have been recently developed to solve these drawbacks, providing accurate and rapid diagnostics at field level or at the site of outbreaks. In this work, a bio-photonic device has been developed for the diagnosis of COVID-19. The device is used with an isothermal system (Easy Loop Amplification based) for the detection of SARS-CoV-2. The performance of the device was evaluated in the detection of a SARS-CoV-2 RNA sample panel, showing an analytical sensitivity comparable to the reference method of quantitative reverse transcription polymerase chain reaction used commercially. In addition, the device was mainly built with simple and low-cost components; therefore, it is possible to obtain a high-efficiency and low-cost instrument. The device excites the sample to be analyzed with a semiconductor laser with a specific wavelength, thus triggering spontaneous emission of the fluorophore bound to the specific probe. The emitted fluorescence is suitably managed by using interferential filters. Under these conditions, a signal is registered and, depending on this level, defines the case as positive or negative. All the analysis is done autonomously inside the developed device through an integrated control system, and it is connected to a portable device to show the results wirelessly.

show abstract

Small form factor flow virometer for SARS-CoV-2

Cited by 3 publications

References 23 publications

A lab-on-chip solution for the detection and suppression of COVID-19 severity

A lab-on-chip solution for the detection and suppression of COVID-19 severity

Saliva Analysis Based on Microfluidics: Focusing the Wide Spectrum of Target Analyte

Point-of-care real-time DNA detection device for SARS-CoV-2 from clinical samples

Contact Info

Product

Resources

About