Small Interfering RNA-Mediated Silencing of Heat Shock Protein 27 (HSP27) Increases Chemosensitivity to Paclitaxel by Increasing Production of Reactive Oxygen Species in Human Ovarian Cancer Cells (HO8910)

Song, Tiefang; Zhang, Z F; Liu, L; Yang, Tingting; Jiang, Jing; Li, Peiling

doi:10.1177/147323000903700512

Cited by 42 publications

(38 citation statements)

References 28 publications

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“…This alternative explanation for taxol action is in perfect agreement with the findings by Song et al (103) and Torres and Horwitz (104) who have shown that different mechanisms can be activated, dependent on the concentration of taxol applied. In line with this finding, Lin et al have shown that low concentrations of taxol cause cell death without affecting microtubules and mitosis (105).…”

Section: Singlet Oxygen-related Aspects Related To the Action Of Taxolsupporting

confidence: 92%

The Antitumor Effect of Singlet Oxygen

Bauer

2016

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Section: Singlet Oxygen-related Aspects Related To the Action Of Taxolsupporting

confidence: 92%

The Antitumor Effect of Singlet Oxygen

Bauer

2016

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“…Approximately half of the differentially expressed proteins identified in this study are related to the organization or activity of the actin cytoskeleton, which may explain the failure of strategies based on changes in the expression of P-glycoprotein and tubulin in clinical treatment. Although alterations of actin associated with drug resistance have been reported, hsp27 and stathmin were also confirmed to be relevant to drug resistance in previous studies (18)(19)(20)(21). Unfortunately, their high expression was not observed in these resistant cell lines.…”

Section: Discussionmentioning

confidence: 92%

Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo

Yin

Mao³

et al. 2012

Oncology Reports

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Abstract. The acquisition of chemoresistance is a major therapeutic obstacle in the clinical treatment of ovarian cancer. Diagnosing chemoresistance in ovarian cancer patients at an early stage is necessary for prognosis, but at present significant proteins related to chemoresistance that may indicate and reverse chemoresistance in human ovarian cancer have not been discovered. In this study, we demonstrated that the protein, phosphorylated cofilin 1 (p-CFL1) correlates with taxol resistance in human ovarian cancer cells. The total proteins of two sensitive (SKOV3 and A2780) and three taxolresistant (SKOV3/TR2500, SKOV3/TR30 and A2780/TR) human ovarian cancer cell lines were isolated by 2-dimensional gel electrophoresis (2-DE). Twenty-two protein spots in all samples were revealed to be significantly different in spot intensity by statistical analysis, 16 of which were identified using matrix-assisted laser desorption ionization-time-of-flightmass spectrometry (MALDI-TOF-MS). Cofilin 1 (CFL1) was selected as a candidate which may play an important role in taxol resistance. Higher expression levels of p-CFL1 in taxolresistant cells were demonstrated. Furthermore, the levels of p-CFL1 in primary human ovarian cancer tissues were shown to be significantly higher in chemoresistant cases compared to those in chemosensitive ones. These findings suggest that p-CFL1 is upregulated in taxol-resistant ovarian cancer and this upregulation is a chara cteristic of taxol resistance both in vitro and in vivo. However, the mechanisms need to be further elucidated.

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“…27 Down-regulation of HSP27 expression increased chemosensitivity of human ovarian cancer cells to paclitaxel and increased paclitaxel-induced apoptosis. 28 Paclitaxel was reported to suppress HSP27 expression in breast cancer cells and enhance the effect of doxorubicin. 29 Heat shock protein 27 down-regulation decreased eukaryotic translation initiation factor 4E and conferred resistance to androgen ablation and chemotherapy in prostate cancer cells.…”

Section: Discussionmentioning

confidence: 99%