Small Intestinal Submucosa as a Bioscaffold for Tissue Regeneration in Defects of the Colonic Wall

Abstract

SIS can be used effectively for patch repair of colonic defects in a porcine model. Distinctive contraction of the reconstructed area and limited architectural regeneration of the bowel wall suggest limitation of morphologic regenerative capacities in large-bowel regeneration.

Pitfalls and Technical Aspects during the Research of Intestinal Anastomotic Healing in Rats

“…The same perioperative conditions were applied in all rats, and the animals experienced the same anastomotic technique either for the small bowel or the colon, differing only in the individually examined variables. Specific data of the different studies have been published elsewhere [10][11][12][13][14][15] .…”

A multi-step method for preparation of porcine small intestinal submucosa (SIS)

“…SIS from swine displays very low immunogenicity without provoking immune-mediated inflammatory reactions when transplanted. To date, SIS has been extensively used as scaffolds for tissue engineering applications of artery [7], bladder [8], intestinal tract [9,10], and tendon [11]. The US FDA has approved its use in human for urogenital procedures, such as hernia repair, cystoplasties, ureteral reconstructions, stress incontinence, peyronie's disease, penile chordee, even urethral reconstruction for hypospadias and strictures [12e16].…”

Anastomotic Sealing by Extracellular Matrices (ECM) Improves Healing of Colonic Anastomoses in the Critical Early Phase

“…Reinforcement of the anastomosis by wrapping it with an artificial or biological graft has been claimed to be useful. 5 A promising approach for protection of gastrointestinal anastomosis is anastomotic sealing with so-called extracellular matrices (ECM). ECM are acellular, collagenous resorbable scaffolds of biological origin.…”

“…Especially small intestinal submucosa (SIS) has been experimentally evaluated for intestinal tissue engineering by our group and others. [5][6][7][8] SIS is a biodegradable, commercially available, acellular, immunologically inert collagen matrix, which is extracted from the submucosal layer of porcine small bowel. Different regulatory proteins which play important roles in the promotion of wound healing have been expressed on SIS.…”