Small intestine submucosa sponge for in vivo support of tissue-engineered bone formation in the presence of rat bone marrow stem cells

Kim, Kyung Sook; Lee, Ju Young; Kang, Young Sook; Kim, E.Sle; Kim, Gyeong Hae; Rhee, Sang Dal; Cheon, Hyae Gyeong; Kim, Jae Ho; Min, Byoung‐Hyun; Lee, Hai Bang; Kim, Moon Suk

doi:10.1016/j.biomaterials.2009.10.020

Cited by 91 publications

(63 citation statements)

References 40 publications

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“…Since it was approved by the US Food and Drug Administration for soft tissue repair in 1999, SIS has been widely used in a variety of tissues and organs, with successful results in tissue regeneration and functional recovery. 23,25,38 As an acellular extracellular matrix, the SIS can not only provide a three-dimensional structure for cell adhesion, growth, and migration, but it also These may signal to the host tissue to promote angiogenesis, cell migration, and differentiation. In this study, the results demonstrated the formation of neo-tissue at the junctions between muscle tissue and the scaffold.…”

Section: Figmentioning

confidence: 99%

“…21,22 SIS is widely applied as a biodegradable scaffold in tissue engineering of various tissues of the urogenital, vascular, and musculoskeletal systems. [22][23][24][25] Various studies have been performed to prove the feasibility of SIS as a tissue engineering scaffold in animal models of rats, dogs, and pigs, and even human patients. [25][26][27][28] Different types of seed cells have been used for repairing abdominal wall defects, including skeletal muscle, skin fibroblasts, and bone marrow stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…[22][23][24][25] Various studies have been performed to prove the feasibility of SIS as a tissue engineering scaffold in animal models of rats, dogs, and pigs, and even human patients. [25][26][27][28] Different types of seed cells have been used for repairing abdominal wall defects, including skeletal muscle, skin fibroblasts, and bone marrow stem cells. 21,25,29 However, there have been no published reports on the use of tenocytes to engineer aponeurosis for potential applications in abdominal wall reconstruction.…”

Section: Introductionmentioning

confidence: 99%

“…[25][26][27][28] Different types of seed cells have been used for repairing abdominal wall defects, including skeletal muscle, skin fibroblasts, and bone marrow stem cells. 21,25,29 However, there have been no published reports on the use of tenocytes to engineer aponeurosis for potential applications in abdominal wall reconstruction. Recent studies have revealed that the tendon and tendon sheath defects can be successfully repaired with a tissue-engineered tendon and tendon sheath using polyglycolic acid fibers and the corresponding cells in a hen model, with good biocompatibility and mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Song

Peng

Liu

et al. 2013

Tissue Engineering Part A

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The repair of abdominal wall defects following surgery remains a difficult challenge. Although multiple methods have been described to restore the integrity of the abdominal wall, there is no clear consensus on the ideal material for reconstruction. This study explored the feasibility of in vivo reconstruction of a rat model of an abdominal wall defect with a composite scaffold of tenocytes and porcine small intestinal submucosa (SIS). In the current study, we created a 2 · 1.5 cm abdominal wall defect in the anterolateral abdominal wall of SpragueDawley rats, which were assigned into three groups: the cell-SIS construct group, the cell-free SIS scaffold group, and the abdominal wall defect group. Tenocytes were obtained from the tendons of rat limbs. After isolation and expansion, cells (2 · 10 7 /mL) were seeded onto the three-layer SIS scaffolds and cultured in vitro for 5 days. Cell-SIS constructs or cell-free constructs were implanted to repair the abdominal wall defects. The results showed that the tenocytes could grow on the SIS scaffold and secreted corresponding matrices. In addition, both scaffolds could repair the abdominal wall defects with no hernia recurrence. In comparison to the cell-free SIS scaffold, the composite scaffold exhibited increased vascular regeneration and mechanical strength. Furthermore, following increased time in vivo, the mechanical strength of the composite scaffold became stronger. The results indicate that the composite scaffold can provide increased mechanical strength that may be suitable for repairing abdominal wall defects.

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Section: Figmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Song

Peng

Liu

et al. 2013

Tissue Engineering Part A

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show abstract

“…132,135 In addition to these native ECM molecules, the collagen fiber orientation that is maintained after the decell process has also attracted attention. 132 Both of these inherent properties have sparked strategies employing SIS as scaffolds in the fields of cardiovascular, [137][138][139][140] bone, [141][142][143] nerve, 144 soft tissue, 86,87,145 and urogenital [146][147][148][149] tissue engineering (Table 6). Currently, SIS is Food and Drug Administration (FDA) approved for several urogenital applications, including hernia repair.…”

Section: Small Intestinal Submucosamentioning

confidence: 99%

Leveraging “Raw Materials” as Building Blocks and Bioactive Signals in Regenerative Medicine

Renth

Detamore

2012

Tissue Engineering Part B: Reviews

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Hierarchical Nanofibrous Microspheres with Controlled Growth Factor Delivery for Bone Regeneration

Jing

Sun

et al. 2015

Adv Healthcare Materials

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The integration of controlled growth factor delivery and biomimetic architecture into a microsphere is a challenging but attractive strategy for developing new injectable biomaterials for tissue engineering. In this work, we developed a unique hierarchical nanosphere-encapsulated-in-microsphere scaffolding system for bone tissue regeneration. First, we synthesized heparin-conjugated gelatin (HG) that provides binding domains for bone morphogenetic protein 2 (BMP2) to stabilize this growth factor, protect it from denaturation and proteolytic degradation, and subsequently prolong its sustained release. Next, we developed a unique approach that includes a water-in-oil-in-oil (W/O/O) double emulsion process and a thermally induced phase separation to encapsulate BMP2-binding HG nanospheres into nanofibrous microspheres. The nanofibrous microsphere was self-assembled from synthetic nanofibers, and had superior surface area, high porosity, low density, and was an excellent carrier to support cell adhesion and tissue in-growth. BMP2 in the hierarchical microsphere was released in a multiple-controlled manner (by the binding with heparin and encapsulation of the nanosphere and microsphere) and retained its high bioactivity. An in vivo calvarial defect model confirmed that this unique hierarchical microsphere was an excellent osteoinductive scaffold for enhanced bone regeneration. By choosing different growth factors, this hierarchical microsphere system can be easily applied to other types of tissue regeneration. Our work expands the ability to develop new injectable biomaterials for advanced regenerative therapies.

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Small intestine submucosa sponge for in vivo support of tissue-engineered bone formation in the presence of rat bone marrow stem cells

Cited by 91 publications

References 40 publications

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Leveraging “Raw Materials” as Building Blocks and Bioactive Signals in Regenerative Medicine

Hierarchical Nanofibrous Microspheres with Controlled Growth Factor Delivery for Bone Regeneration

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