Small molecule detection in solution via the size contraction response of aptamer functionalized nanoparticles

Alsager, Omar A.; Kumar, Shalen; Willmott, Geoff R.; McNatty, Kenneth P.; Hodgkiss, Justin M.

doi:10.1016/j.bios.2014.02.004

Cited by 100 publications

(97 citation statements)

References 36 publications

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“…The monomer 3-pyrrolyacrylic acid (PAA) was synthesized as previously reported by our group (Peng et al, 2007). The sequence of the 75-mer E2 aptamer used for sensing was taken from (Alsager et al, 2014) and was purchased with amino modification on the 5' end from Sigma-Aldrich. All sequences are provided in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

“…Binding signals can then be transduced via colorimetric (Barthelmebs et al, 2011;Y. S. Kim et al, 2010;Zheng et al, 2011), fluorescence (Sassolas et al, 2011;Yildirim et al, 2012a), size (Alsager et al, 2014), and electrochemical responses (Y.-J. Kim et al, 2007;Lin et al, 2012a;Xue et al, 2012;Zayats et al, 2006).…”

Section: Introductionmentioning

confidence: 98%

“…We selected a 75-mer aptamer that we have previously shown to adopt a compact conformation when binding to its target molecule, 17β-estradiol (E2) (Alsager et al, 2014). There is a strong demand for sensors that rapidly and accurately quantify hormones on the extremely low concentration range at which they elicit biological responses, including as environmental contaminants (Auriol et al, 2006;Campbell et al, 2006;Geyer et al, 2000) and fertility markers.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Label-free electrochemical aptasensor for femtomolar detection of 17β-estradiol

Zhu

Alsager

Kumar

et al. 2015

Biosensors and Bioelectronics

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Label-free electrochemical aptasensor for femtomolar detection of 17β-estradiol

Zhu

Alsager

Kumar

et al. 2015

Biosensors and Bioelectronics

Self Cite

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“…To facilitate sample handling and assay speed some RPS strategies have included nanomaterials by either immobilising the target analytes onto their surface facilitating an analyte induced aggregation 35,36 , or measuring the particle translocation speed/ frequency upon the binding of the analyte 11,30,[37][38][39][40] . With the charge of the particle being a contributing factor in pore translocation speed and frequency, the use of DNA modified materials, and pores, has become increasingly popular 11,30,[41][42][43] .…”

Section: Assay 1 -Measuring Translocation Times From Resistive Pulsesmentioning

confidence: 99%

Protein detection using tunable pores: resistive pulses and current rectification

et al. 2016

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We present the first comparison between assays that use resistive pulses or rectification ratios on a tunable pore platform. We compare their ability to quantify the cancer biomarker Vascular Endothelial Growth Factor (VEGF). The first assay measures the electrophoretic mobility of aptamer modified nanoparticles as they traverse the pore. By controlling the aptamer loading on the particle surface, and measuring the speed of each translocation event we are able to observe a change in velocity as low as 18 pM. A second non-particle assay exploits the current rectification properties of conical pores. We report the first use of Layer-by-Layer (LbL) assembly of polyelectrolytes onto the surface of the polyurethane pore. The current rectification ratios demonstrate the presence of the polymers, producing pH and ionic strength-dependent currents. The LbL assembly allows the facile immobilisation of DNA aptamers onto the pore allowing a specific dose response to VEGF. Monitoring changes to the current rectification allows for a rapid detection of 5 pM VEGF. Each assay format offers advantages in their setup and ease of preparation but comparable sensitivities.

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“…Interestingly, they were able to observe binding of two proteins to their specific aptamers simultaneously. Alsager and co-workers [44] have designed a simple sensor to detect 17β-estradiol utilising an aptamer attached to the surface of 217nm sized carboxylated polystyrene nanoparticles. The interactions between the aptamer conjugated nanoparticle and the target molecule were studied by TRPS and DLS.…”

Section: Trps In the Analysis Of Nanoparticle Bound Aptamer Interactimentioning

confidence: 99%

Tunable Resistive Pulse Sensing: Potential Applications in Nanomedicine

Sivakumaran

Platt

2016

Nanomedicine (Lond.)

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An accurate characterisation of nanomaterials used in clinical diagnosis and therapeutics is of paramount importance to realise the full potential of nanotechnology in medicine and to avoid unexpected and potentially harmful toxic effects due to these materials. A number of technical modalities are currently in use to study the physical, chemical and biological properties of nanomaterials but they all have advantages and disadvantages. In this review, we discuss the potential of a relative newcomer, Tunable Resistive Pulse Sensing (TRPS), for the characterisation of nanomaterials and its applications in nanodiagnostics.

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Small molecule detection in solution via the size contraction response of aptamer functionalized nanoparticles

Cited by 100 publications

References 36 publications

Label-free electrochemical aptasensor for femtomolar detection of 17β-estradiol

Label-free electrochemical aptasensor for femtomolar detection of 17β-estradiol

Protein detection using tunable pores: resistive pulses and current rectification

Tunable Resistive Pulse Sensing: Potential Applications in Nanomedicine

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