Small Molecule Immunosensing Using Surface Plasmon Resonance

Mitchell, John S.

doi:10.3390/s100807323

Cited by 145 publications

(105 citation statements)

References 105 publications

(150 reference statements)

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“…By providing a spatially coherent peptide spacer, these problems may be overcome as well as an improved degree of receptor orientation. The effect of steric hindrance has been demonstrated as a critical parameter in biosensor signal generation in previous studies [22][23][24][25]. It is hypothesised that the use of nanobodies will highlight the importance of orientation of the bioreceptor.…”

Section: Introductionmentioning

confidence: 99%

The development and optimisation of nanobody based electrochemical immunosensors for IgG

Goode

Dillon

Millner

2016

Sensors and Actuators B: Chemical

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Article:Goode, J orcid.org/0000-0002-3642-1291, Dillon, G and Millner, PA orcid.org/0000-0002-4813-4302 (2016 ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. This has led to the development of alternative immuno-reagents such as non-antibody binding proteins (NABPs). These are low molecular weight proteins which largely avoid the aforementioned advantages of antibodies. They are commonly produced by bacteria enabling the use of DNA technology to manipulate bioreceptors at the molecular level.Single chain VHHs (commonly known as nanobodies) are an antibody derived NABP adapted from camelid heavy chain antibodies which are the isolated binding domain. Whilst nanobodies have been used for diagnostic and therapeutic applications, they have limited demonstration in biosensors.In this study, both antibodies and nanobodies were used to construct a biosensor. In addition nanobody performance was optimised by introducing a novel peptide spacer. The role of nanobody orientation and spacing was thus investigated and spacer length was optimised, leading to an increase in the sensitivity of the biosensor. Highlights: Nanobodies have been used on impedimetric immunosensors to detect rabbit IgG  Unmodified nanobody sensors displayed a decrease in resistance upon analyte recognition.  Nanobodies were modified with a peptide spacer, effectively reversing this phenomena.  The use of a spacer also increased the sensitivity of the immunosensor.

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Section: Introductionmentioning

confidence: 99%

The development and optimisation of nanobody based electrochemical immunosensors for IgG

Goode

Dillon

Millner

2016

Sensors and Actuators B: Chemical

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“…Огляд Jona Mitchela [110] досліджує технологію застосування ППР для іммунологічного обстеження молекул антиге-нів з низькою молекулярною вагою, зокрема, мікотоксинів. Розглядаються матричні ефек-ти, викликані вимірюваннями в хімічно склад-них зразках, побудова стійкої поверхні сенсора та розвиток мультикомплексних випробувань, здатних до виявлення кількох токсинів одразу.…”

Section: застосування біосенсорів для моніторин-гу мікотоксинівunclassified

Application of Modern Biosensors Methods in Ecotoxicological Monitoring of Some Toxins of Natural (Micotoxins) and Antropogenic (Pesticides) Origin. Part 1. Micotoxins.

Гойстер¹,

Дзядевич²,

Мінченко³

2013

SEMST

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“…Over the two past decades, a large number of immunosensors targeting individual pollutants or groups of pollutants and based on these different configurations have been reported. Recent developments have been focused on label free immunosensors using EIS and SPR detection (Mitchell, 2010;Prodromidis, 2010) as well as on improvements in antibody design (Conry et al, 2009).…”

Section: Immunosensorsmentioning

confidence: 99%