Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity

Abstract

The set-up of highly sensitive detection tools to evaluate lipase activity remains a central goal in different fields. In this context, we proposed new chemiluminescent 1,2-dioxetane luminophores, sharing an octanoyl triggerable group, to monitor lipase activity. We herein report the synthesis and both the evaluation of their luminescence emission profile and their enzyme–substrate specificity, generated by three different commercial lipases (Candida cylindracea, Pseudomonas fluorescens, and Mucor miehei) and one esterase (porcine liver esterase, PLE, as a literature control). Remarkably, the present study confirmed the applicability of these 1,2-dioxetane luminophores as (i) highly efficient, broad-range, chemiluminescent probes for the detection and the enzymatic activity evaluation of lipases and as (ii) promising candidates for the future development of both flash- and glow-type luminescence assays.

Recent Advances in the Development of Non‐Invasive Imaging Probes for Cancer Immunotherapy

“…Chemiluminescence (CL), on the other hand, is the emission of light from an organic molecule formed in its excited state during a chemical reaction that does not require a secondary enzymatic reaction to produce a photon after uncaging (Figure 9D). [129][130][131][132][133][134] Representative CL substrates include luminol, acridinium ester, peroxyoxalate, 1,2dioxetane and their derivatives. The optimization of 1,2dioxetane to improve quantum efficiency and aqueous solubility has produced a versatile scaffold for use in cellular and in vivo applications.…”