Small RNA analysis of Potato Spindle Tuber Viroid infected Phelipanche ramosa

Ivanova, Desislava; Milev, Ivan; Vachev, Tihomir; Baev, Vesselin; Yahubyan, Galina; Minkov, Georgi; Gozmanova, Mariyana

doi:10.1016/j.plaphy.2013.11.019

Cited by 23 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Silencing is triggered by the conversion of long double-stranded RNAs into small RNA duplexes of 21 and 24 nucleotides (Hamilton et al, 2002). The accumulation of such viroid-derived small RNAs (vdsRNAs) has been extensively studied in different viroid-host combinations (Bolduc et al, 2010;Ivanova et al, 2014;Tsushima et al, 2015), and multiple plant Argonaute proteins bind these vdsRNAs (Minoia et al, 2015). These observations support the hypotheses that viroids infection trigger RNA silencing and that RNAi might play a role in symptom induction.…”

Section: Introductionmentioning

confidence: 56%

Small RNA Derived from the Virulence Modulating Region of the Potato spindle tuber viroid Silences callose synthase Genes of Tomato Plants

et al. 2015

View full text Add to dashboard Cite

The tomato (Solanum lycopersicum) callose synthase genes CalS11-like and CalS12-like encode proteins that are essential for the formation of callose, a major component of pollen mother cell walls; these enzymes also function in callose formation during pathogen infection. This article describes the targeting of these callose synthase mRNAs by a small RNA derived from the virulence modulating region of two Potato spindle tuber viroid variants. More specifically, viroid infection of tomato plants resulted in the suppression of the target mRNAs up to 1.5-fold, depending on the viroid variant used and the gene targeted. The targeting of these mRNAs by RNA silencing was validated by artificial microRNA experiments in a transient expression system and by RNA ligase-mediated rapid amplification of cDNA ends. Viroid mutants incapable of targeting callose synthase mRNAs failed to induce typical infection phenotypes, whereas a chimeric viroid obtained by swapping the virulence modulating regions of a mild and a severe variant of Potato spindle tuber viroid greatly affected the accumulation of viroids and the severity of disease symptoms. These data provide evidence of the silencing of multiple genes by a single small RNA derived from a viroid.

show abstract

Section: Introductionmentioning

confidence: 56%

Small RNA Derived from the Virulence Modulating Region of the Potato spindle tuber viroid Silences callose synthase Genes of Tomato Plants

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Additionally, the mechanical inoculations of six potential viroid hosts did not result in positive detection (Table ). Table presents the outcomes of this study, together with a review of all previously published pospiviroid host studies (Antignus et al ., ; Matousek et al ., ; Ivanova et al ., ). In total, 40 plant species from 17 families have been inoculated with pospiviroids, either biolistically (Matousek et al ., ) or mechanically, using carborundum (Antignus et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…In addition to these putative hosts, a number of plants also gave faint positive results after reverse transcription (RT)‐PCR, that is Galinsoga ciliata L. (hairy galinsoga, shaggy soldier) and Amaranthus retroflexus L. (redroot pigweed). Recently, the parasitic weed Phelipanche ramosa L. Pomel (hemp broomrape) has been reported as a host plant for PSTVd under experimental conditions (Ivanova et al ., ).…”

Section: Introductionmentioning

confidence: 97%

The role of weeds in the epidemiology of pospiviroids

Bogaert

Smagghe

Jonghe³

2015

Weed Research

View full text Add to dashboard Cite

Many weeds that are closely associated with horticultural activities are known as natural reservoirs of plant viruses. However, whether these weeds can also serve as hosts of pospiviroids is not well known. Pospiviroids are naked, non-coding RNA pathogens that cause severe economic damage in many solanaceous crops. In this study, we examined the overall risk of pospiviroid spreading from weeds to economically important crops, by combining the results from previous inoculation studies with new results coming from a survey, a contact experiment and an inoculation experiment. A survey of commercial ornamental glasshouses revealed that ornamental plants mainly belonging to the Solanaceae harbour pospiviroids, in contrast to weed species sampled in the same places. No new weed hosts could be identified after testing weeds that grew in contact with Tomato apical stunt viroid (TASVd)-infected plants of tomato (Solanum lycopersicum) and jasmine nightshade (Solanum jasminoides) in an experimental glasshouse. Finally, in mechanical inoculation experiments with TASVd, none of the six tested weed species were determined to be a host at 6 weeks after inoculation. Commonly occurring weed species therefore do not appear to play a significant role as reservoir hosts for pospiviroids. This does not rule out other potential weed hosts that have not yet been tested. Inoculation studies should include rigorous experimental protocols with a sufficient number of replicated as well as adequate positive controls. The information gained through this study may prove useful in future risk assessments for the pospiviroid group

show abstract

“…miR166h‐p5 is produced from the 5′ arm of the MIR166h precursor and is significantly up‐regulated in PVX‐infected N. benthamiana plants. This miRNA has not been reported previously to respond to viral infection, although miR166h, produced from the 3′ arm of the MIR166 precursor, shows changed expression in Potato spindle tuber viroid (PSTVd)‐infected Phelipanche ramose and Mungbean yellow mosaic India virus (MYMIV)‐infected Vigna mungo (Ivanova et al ., ; Kundu et al ., ). In a previous study, it has been reported that RSV infection induces the expression of novel miRNAs in a phased pattern from several conserved miRNA precursors, and enhances the accumulation of some rice miRNA*s, but not their corresponding miRNAs (Du et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Suppression of nbe‐miR166h‐p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation

Wang

Cui

et al. 2018

Molecular Plant Pathology

View full text Add to dashboard Cite

MicroRNAs (miRNAs) play essential roles in plant development. There is increasing evidence that changed expression of miRNAs in virus-infected plants contributes to the development of viral symptoms. Here, we analysed the altered expression of miRNAs of Nicotiana benthamiana in response to Potato virus X (PVX) by Illumina Solexa sequencing. One of the 21 miRNAs significantly affected, nbe-miR166h-p5, was closely associated with viral symptoms. Using the Tobacco rattle virus-based miRNA suppression (VbMS) system, we found that the suppression of nbe-miR166h-p5 in plants caused leaves to turn dark green with increased chlorophyll. When PVX was inoculated on nbe-miR166h-p5-suppressed plants, the leaf yellowing symptom of PVX was largely attenuated with less reduction in chlorophyll content, and the accumulation of PVX was decreased. nbe-miR166h-p5 was also up-regulated in plants infected by Turnip mosaic virus (TuMV), and its suppression attenuated the leaf yellowing symptom of TuMV and decreased viral accumulation. Three potential targets of nbe-miR166h-p5 were identified. The results indicate the association of nbe-miR166h-p5 with symptoms of PVX and also with those of TuMV, providing useful information on the relationship between miRNA and viral infection.

show abstract

Small RNA analysis of Potato Spindle Tuber Viroid infected Phelipanche ramosa

Cited by 23 publications

References 37 publications

Small RNA Derived from the Virulence Modulating Region of the Potato spindle tuber viroid Silences callose synthase Genes of Tomato Plants

Small RNA Derived from the Virulence Modulating Region of the Potato spindle tuber viroid Silences callose synthase Genes of Tomato Plants

The role of weeds in the epidemiology of pospiviroids

Suppression of nbe‐miR166h‐p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation

Contact Info

Product

Resources

About