2009
DOI: 10.1073/pnas.pnas.0910308106
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Small RNA as global regulator of carbon catabolite repression in Pseudomonas aeruginosa

Abstract: In the metabolically versatile bacterium Pseudomonas aeruginosa, the RNA-binding protein Crc is involved in catabolite repression of a range of degradative genes, such as amiE (encoding aliphatic amidase). We found that a CA-rich sequence (termed CA motif) in the amiE translation initiation region was important for Crc binding. The small RNA CrcZ (407 nt) containing 5 CA motifs was able to bind the Crc protein with high affinity and to remove it from amiE mRNA in vitro. Overexpression of crcZ relieved cataboli… Show more

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Cited by 150 publications
(402 citation statements)
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“…cAMP and its respective cAMP-binding protein do not seem to be responsible for catabolite repression mechanisms in this genus. 40 A master regulator of carbon metabolism in Pseudomonas is an RNA-binding protein named Crc, which, under repressive conditions, inhibits the translation of mRNAs by binding to a sequence just downstream of the AUG start codon (Figure 2). 41 Conversely, under nonrepressive conditions, a small RNA named CrcZ binds to the Crc protein to relieve mRNAs from catabolite repression.…”
Section: Carbon Source Regulation Of Antibiotic Production In Gram-nementioning
confidence: 99%
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“…cAMP and its respective cAMP-binding protein do not seem to be responsible for catabolite repression mechanisms in this genus. 40 A master regulator of carbon metabolism in Pseudomonas is an RNA-binding protein named Crc, which, under repressive conditions, inhibits the translation of mRNAs by binding to a sequence just downstream of the AUG start codon (Figure 2). 41 Conversely, under nonrepressive conditions, a small RNA named CrcZ binds to the Crc protein to relieve mRNAs from catabolite repression.…”
Section: Carbon Source Regulation Of Antibiotic Production In Gram-nementioning
confidence: 99%
“…A two-component system CbrA/CbrB, which is sensitive to the carbon source, controls the expression of CrcZ. 40 In the same manner, other RNA-binding proteins such as RsmA (repressor of secondary metabolism) from Pseudomonas fluorescens avoid translation by attachment to mRNAs related to the synthesis of extracellular antifungal secondary metabolites. Translation of mRNAs can continue when the cell expresses high quantities of small RNAs that compete for binding to RsmA, which is similar to the carbon storage regulator, CsrA from E. coli and the regulation of sRNA expression is promoted by a two-component system named GacS/GacA.…”
Section: Carbon Source Regulation Of Antibiotic Production In Gram-nementioning
confidence: 99%
“…Crc is an RNA-binding protein that recognizes specific sites located at the translation initiation regions of target mRNAs, inhibiting translation (30 -32). In Pseudomonas aeruginosa, the levels of free Crc in the cell are regulated by a small RNA named CrcZ, which binds to and sequesters Crc (33). CrcZ expression in turn varies depending on the medium composition.…”
mentioning
confidence: 99%
“…While sRNA Spot 42 mediates CCR in E. coli, sRNA, CrcZ helps in the reversal of CCR in Pseudomonas spp. (39).…”
Section: Role Of Srnas In Ccrmentioning
confidence: 99%