Small RNA mediated regulation of seed germination

Das, Shabari Sarkar; Karmakar, Prakash; Nandi, Asis Kumar; Sanan-Mishra, Neeti

doi:10.3389/fpls.2015.00828

Cited by 46 publications

(41 citation statements)

References 53 publications

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“…MicroRNAs act as a kind of small and noncoding nucleotide RNAs, which control the expression of genes via directly and indirectly interacting with the 3′-untranslated region (UTR) of the corresponding target mRNA to affect the transcription process and/or mediate the degradation of relevant mRNAs [6,7]. Notably, it is reported that miRNA families play an essential role in a couple of oncogenesis, including survival, growth, apoptosis, migration, and metastasis [8].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: miR-124 Acts as a Tumor Suppressor in Glioblastoma via the Inhibition of Signal Transducer and Activator of Transcription 3

Huang

et al. 2016

Mol Neurobiol

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MicroRNAs are important regulators of multiple cellular processes, and aberrant miRNA expression has been observed in human glioblastoma (GBM). The present study was to evaluate the level of miR-124 and signal transducer and activator of transcription 3 (STAT3) in GBM tissues and cells. We further investigated the molecular mechanisms of miR-124 and STAT3 in GBM cell lines U87 and U251. Here, we found that miR-124 expression was downregulated in GBM tissues and U87 and U251 cells (all p < 0.001) but not associated with blood routine (RBC, WBC count, etc.) and liver and renal function indicators (all p > 0.05). By contrast, STAT3 was upregulated. Furthermore, the expression of miR-124 was inversely proportional to that of STAT3 mRNA or protein (p = 0.013, p = 0.015, respectively). In vitro studies demonstrated that the overexpression of miR-124 played a suppressor role in the proliferation of U87 and U251 cells and promoted cell apoptosis. Luciferase reporter assays confirmed that miR-124 binding to the 3'-UTR regions of STAT3 inhibited the expression of STAT3 in U87 and U251 cells. However, the inhibitor of miR-124 promoted the expression of STAT3 and cell proliferation. In conclusion, our data suggest that miR-124 may have a potential role in treatment of GBM patients and that miR-124 is a novel regulator of invasiveness and tumorigenicity in GBM cells by targeting STAT3. The miR-124/STAT3 pathway may be a useful therapeutic agent in GBM patients.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: miR-124 Acts as a Tumor Suppressor in Glioblastoma via the Inhibition of Signal Transducer and Activator of Transcription 3

Huang

et al. 2016

Mol Neurobiol

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“…The list of differentially expressed NPs included several key TFs, out of which bZIP TF TRAB1 and trihelix TF ASIL2‐like were late stress‐responsive, up‐regulated during severe dehydration. TRAB1 regulates ABA signalling and ASIL2 plays key role in seed maturation (Das, Karmakar, Nandi, & Sanan‐Mishra, ; Hobo, Kowyama, & Hattori, ). TF BTF3 homolog 4‐like was down‐regulated at 72 hr dehydration.…”

Section: Resultsmentioning

confidence: 99%

“…To identify TFs, we carried out a sequence blast of the NPs with that of the chickpea TF database TransDB (Gayali et al, 2016). The analysis led to the identification of as many as 200 TFs, of which 34 are yet to be characterized (Table S8) (Das, Karmakar, Nandi, & Sanan-Mishra, 2015;Hobo, Kowyama, & Hattori, 1999). TF BTF3 homolog 4-like was down-regulated at 72 hr dehydration.…”

Section: Identification Of Tfsmentioning

confidence: 99%

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Barua

Lande

Subba

et al. 2018

Plant Cell & Environment

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Nonavailability of water or dehydration remains recurring climatic disorder affecting yield of major food crops, legumes in particular. Nuclear proteins (NPs) and phosphoproteins (NPPs) execute crucial cellular functions that form the regulatory hub for coordinated stress response. Phosphoproteins hold enormous influence over cellular signalling. Four-week-old seedlings of a grain legume, chickpea, were subjected to gradual dehydration, and NPs were extracted from unstressed control and from 72- and 144-hr stressed tissues. We identified 4,832 NPs and 478 phosphosites, corresponding to 299 unique NPPs involved in multivariate cellular processes including protein modification and gene expression regulation, among others. The identified proteins included several novel kinases, phosphatases, and transcription factors, besides 660 uncharacterized proteins. Spliceosome complex and splicing related proteins were dominant among differentially regulated NPPs, indicating their dehydration modulated regulation. Phospho-motif analysis revealed stress-induced enrichment of proline-directed serine phosphorylation. Association mapping of NPPs revealed predominance of differential phosphorylation of spliceosome and splicing associated proteins. Also, regulatory proteins of key processes viz., protein degradation, regulation of flowering time, and circadian clock were observed to undergo dehydration-induced dephosphorylation. The characterization of novel regulatory proteins would provide new insights into stress adaptation and enable directed genetic manipulations for developing climate-resilient crops.

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“…Studies in Arabidopsis show that microRNAs (miRNAs) act as both activators and repressors of seed germination and dormancy by interacting with ABA and GA signaling (Martin et al, 2010;Das et al, 2015). Specifically, miR159 regulates mRNA encoding GA-MYB transcription factors, which affect GA-mediated programmed cell death in the aleurone layer, a developmental process that is important for seed germination (Reyes and Chua, 2007;Alonso-Peral et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Wheat miR9678 Affects Seed Germination by Generating Phased siRNAs and Modulating Abscisic Acid/Gibberellin Signaling

et al. 2018

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Seed germination is important for grain yield and quality and rapid, near-simultaneous germination helps in cultivation; however, cultivars that germinate too readily can undergo preharvest sprouting (PHS), which causes substantial losses in areas that tend to get rain around harvest time. Moreover, our knowledge of mechanisms regulating seed germination in wheat () remains limited. In this study, we analyzed function of a wheat-specific microRNA 9678 (miR9678), which is specifically expressed in the scutellum of developing and germinating seeds. Overexpression of miR9678 delayed germination and improved resistance to PHS in wheat through reducing bioactive gibberellin (GA) levels; miR9678 silencing enhanced germination rates. We provide evidence that miR9678 targets a long noncoding RNA () and triggers the generation of phased small interfering RNAs that play a role in the delay of seed germination. Finally, we found that abscisic acid (ABA) signaling proteins bind the promoter of miR9678 precursor and activate its expression, indicating that miR9678 affects germination by modulating the GA/ABA signaling.

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Small RNA mediated regulation of seed germination

Cited by 46 publications

References 53 publications

RETRACTED ARTICLE: miR-124 Acts as a Tumor Suppressor in Glioblastoma via the Inhibition of Signal Transducer and Activator of Transcription 3

RETRACTED ARTICLE: miR-124 Acts as a Tumor Suppressor in Glioblastoma via the Inhibition of Signal Transducer and Activator of Transcription 3

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Wheat miR9678 Affects Seed Germination by Generating Phased siRNAs and Modulating Abscisic Acid/Gibberellin Signaling

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