Small-scale temporal and spatial variations in protistan community composition at the San Pedro Ocean Time-series station off the coast of southern California

Lie, Alle A. Y.; Kim, Diane Y.; Schnetzer, Astrid; Caron, David A.

doi:10.3354/ame01652

Cited by 25 publications

(31 citation statements)

References 79 publications

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“…2). The monthly and seasonal variability in standing stocks of microbial biomass is consistent with previously reported values of monthly-to-interannual variability observed at the sampling site, and even small-scale temporal and spatial variability (Chow et al, 2013;Kim et al, 2013;Lie et al, 2013).…”

Section: The Biological Structure Of the Plankton Community At The Spsupporting

confidence: 90%

Planktonic food web structure at a coastal time-series site: I. Partitioning of microbial abundances and carbon biomass

Caron¹,

Connell²,

Schaffner³

et al. 2017

Deep Sea Research Part I: Oceanographic Research Papers

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Biogeochemistry in marine plankton communities is strongly influenced by the activities of 38 microbial species. Understanding the composition and dynamics of these assemblages is 39 essential for modeling emergent community-level processes, yet few studies have examined all 40 of the biological assemblages present in the plankton, and benchmark data of this sort from time-41 series studies are rare. Abundance and biomass of the entire microbial assemblage and 42 mesozooplankton (>200 μm) were determined vertically, monthly and seasonally over a 3-year 43 period at a coastal time-series station in the San Pedro Basin off the southwestern coast of the USA. All compartments of the planktonic community were enumerated (viruses in the femtoplankton size range [0.02-0.2 μm], bacteria + archaea and cyanobacteria in the picoplankton size range [0.2-2.0 μm] phototrophic and heterotrophic protists in the nanoplanktonic [2-20 μm] and microplanktonic [20-200 μm] size ranges, and mesozooplankton [>200 μm]. Carbon biomass of each category was estimated using standard conversion factors. Plankton abundances varied over seven orders of magnitude across all categories, and total carbon biomass averaged approximately 60 μg C l-1 in surface waters of the 890 m water column over the study period. Bacteria + archaea comprised the single largest component of biomass (>1/3 of the total), with the sum of phototrophic protistan biomass making up a similar proportion. Temporal variability at this subtropical station was not dramatic. Monthly depthspecific and depth-integrated biomass varied 2-fold at the station, while seasonal variances were generally <50%. This study provides benchmark information for investigating long-term environmental forcing on the composition and dynamics of the microbes that dominate food web structure and function at this coastal observatory.

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Section: The Biological Structure Of the Plankton Community At The Spsupporting

confidence: 90%

Planktonic food web structure at a coastal time-series site: I. Partitioning of microbial abundances and carbon biomass

Caron¹,

Connell²,

Schaffner³

et al. 2017

Deep Sea Research Part I: Oceanographic Research Papers

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show abstract

“…Finally, in order to define the variables that were the most important in structuring the COIA scatterplot, Spearman's correlation coefficients were calculated between all variables and COIA coordinates. Environmental and abundance data were log(x + 1) transformed to ensure normal distribution (Legendre & Legendre 1998), and environmental data were standardized to render them dimensionless (z-scores; Kenkel 2006).…”

Section: Discussionmentioning

confidence: 99%

Small-scale variability of protistan planktonic communities relative to environmental pressures and biotic interactions at two adjacent coastal stations

Genitsaris¹,

Monchy²,

Breton³

et al. 2016

Mar. Ecol. Prog. Ser.

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The aim of this study was to analyze planktonic protistan assemblages under different environmental pressures at 2 adjacent coastal stations (inshore and offshore; ~6.4 km apart) in the eastern English Channel (EEC). Samples were collected between March 2012 and June 2013, and analyzed using bioinformatic analysis and tag pyrosequencing of the V2−V3 hypervariable region of the 18S rRNA gene. In addition to the taxonomic composition of the protistan communities, the detected operational taxonomic units (OTUs) were sorted into 6 major functional groups based on their trophic roles in marine systems. Comparisons of 13 environmental factors, including physical and chemical variables, indicated significant differences between the 2 stations. However, the only significant relationship between environmental pressures and protistan community structure was detected when the trophic role of the OTUs was considered at the inshore station. In order to interpret differences in community structure, the effect of biotic inter actions was investigated through the examination of the co-occurrence networks of the protistan communities at both stations. In terms of the number of edges and connectivity of nodes, the analysis showed that the inshore station had more complex associations between OTUs than the offshore station. This strongly suggests that due to the higher and more variable environmental pressures the inshore station comparatively receives, its protistan community has developed a greater complexity of biotic connections. This in turn reflects the rapid responses of trophic interactions within the entire microbial community.

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“…These results provided additional support that rRNA results offered a clearer approach to follow microbial community responses to the light-dark cycle. Previous DNA-based work at a coastal time-series station found that some spatial (within 5 km) and temporal (once daily) variability during sample collection did not dramatically change long-term (e.g., monthly or seasonal) ecological interpretations (Lie et al, 2013;Needham et al, 2017). Those studies, based on DNA sequencing, have provided estimates of total community diversity suitable to detect monthly or seasonal shifts, while results derived here from RNA sequencing better reflect the short-term temporal dynamics in the microbial community.…”

Section: Total Species Richness and Community Composition Based On Rdmentioning

confidence: 91%

A Hard Day's Night: Diel Shifts in Microbial Eukaryotic Activity in the North Pacific Subtropical Gyre

Connell

Mesrop

et al. 2018

Front. Mar. Sci.

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Molecular analysis revealed diel rhythmicity in the metabolic activity of single-celled microbial eukaryotes (protists) within an eddy in the North Pacific Subtropical Gyre (ca. 100 km NE of station ALOHA). Diel trends among different protistan taxonomic groups reflected distinct nutritional capabilities and temporal niche partitioning. Changes in relative metabolic activities among phototrophs corresponded to the light cycle, generally peaking in mid-to late-afternoon. Metabolic activities of protistan taxa with phagotrophic ability were higher at night, relative to daytime, potentially in response to increased availability of picocyanobacterial prey. Tightly correlated Operational Taxonomic Units throughout the diel cycle implicated the existence of parasitic and mutualistic relationships within the microbial eukaryotic community, underscoring the need to define and include these symbiotic interactions in marine food web descriptions. This study provided a new high-resolution view into the ecologically important interactions among primary producers and consumers that mediate the transfer of carbon to higher trophic levels. Characterizations of the temporal dynamics of protistan activities contribute knowledge for predicting how these microorganisms respond to environmental forcing factors.

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Small-scale temporal and spatial variations in protistan community composition at the San Pedro Ocean Time-series station off the coast of southern California

Cited by 25 publications

References 79 publications

Planktonic food web structure at a coastal time-series site: I. Partitioning of microbial abundances and carbon biomass

Planktonic food web structure at a coastal time-series site: I. Partitioning of microbial abundances and carbon biomass

Small-scale variability of protistan planktonic communities relative to environmental pressures and biotic interactions at two adjacent coastal stations

A Hard Day's Night: Diel Shifts in Microbial Eukaryotic Activity in the North Pacific Subtropical Gyre

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