Small‐Sized Carbon Nanohorns Enabling Cellular Uptake Control

Zhang, Minfang; Zhou, Xin; Iijima, Sumio; Yudasaka, Masako

doi:10.1002/smll.201102595

Cited by 43 publications

(42 citation statements)

References 61 publications

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“…7 The previous studies on the interaction between SWCNHs and cells have mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells, although epithelial cell membranes as the typical biological barrier constitute the prime obstacle for the transport of therapeutic agents. 14,15 In light of these previous findings, this study focused on characterizing the effect of this novel material on the polar epithelium and examining its ability to modulate transcellular transport and its potential in drug delivery.…”

Section: Discussionmentioning

confidence: 99%

“…Until now, studies at the cellular level on SWCNHs were mainly focused on the interaction between SWCNHs and non-polarized cells particularly macrophages, and have mostly emphasized on the cellular uptake and intracellular trafficking, but seldom on transportation. [14][15][16][17][18][19][20][21] It is worth noting that, depending on the routes of administration, nanocarriers may have to cross different physiological barriers in their journey towards their target. 22,23 These barriers are mainly polarized epithelia, such as the gastrointestinal tract, the lungs, and the blood-brain barrier.…”

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confidence: 99%

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The interactions of single-wall carbon nanohorns with polar epithelium

Shi¹,

Shi

Li³

et al. 2017

IJN

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Single-wall carbon nanohorns (SWCNHs), which have multitudes of horn interstices, an extensive surface area, and a spherical aggregate structure, offer many advantages over other carbon nanomaterials being used as a drug nanovector. The previous studies on the interaction between SWCNHs and cells have mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells. Polar epithelium as a typical biological barrier constitutes the prime obstacle for the transport of therapeutic agents to target site. This work tried to explore the permeability of SWCNHs through polar epithelium and their abilities to modulate transcellular transport, and evaluate the potential of SWCNHs in drug delivery. Madin-Darby canine kidney (MDCK) cell monolayer was used as a polar epithelial cell model, and as-grown SWCNHs, together with oxidized and fluorescein isothiocyanate-conjugated bovine serum albumin-labeled forms, were constructed and comprehensively investigated in vitro and in vivo. Various methods such as transmission electron microscopy and confocal imaging were used to visualize their intracellular uptake and localization, as well as to investigate the potential transcytotic process. The related mechanism was explored by specific inhibitors. Additionally, fast multispectral optoacoustic tomography imaging was used for monitoring the distribution and transport process of SWCNHs in vivo after oral administration in nude mice, as an evidence for their interaction with the intestinal epithelium. The results showed that SWCNHs had a strong bioadhesion property, and parts of them could be uptaken and transcytosed across the MDCK monolayer. Multiple mechanisms were involved in the uptake and transcytosis of SWCNHs with varying degrees. After oral administration, oxidized SWCNHs were distributed in the gastrointestinal tract and retained in the intestine for up to 36 h probably due to their surface adhesion and endocytosis into the intestinal epithelium. Overall, this comprehensive investigation demonstrated that SWCNHs can serve as a promising nanovector that can cross the barrier of polar epithelial cells and deliver drugs effectively.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

The interactions of single-wall carbon nanohorns with polar epithelium

Shi¹,

Shi

Li³

et al. 2017

IJN

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“…107,108 Smaller sized SWNH aggregates, which can be obtained by adjusting the conditions of synthesis or applying postsynthetic separation techniques, could possibly be used to overcome these problems. 109,110 NANODIAMONDS AND POROUS CARBON NANOSPHERES FOR DRUG DELIVERY Nanodiamonds for anticancer drug delivery Nanodiamonds were first produced decades ago, but they did not gain attention as novel agents for biological applications, such as drug delivery, biolabeling and sensing, until recently. 16,17 As this review focuses on nano-therapeutic applications, we will only discuss studies that apply nanodiamonds for the cellular delivery of various therapeutic agents.…”

Section: Figurementioning

confidence: 99%

Therapeutic applications of low-toxicity spherical nanocarbon materials

Wang

et al. 2014

NPG Asia Mater

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Nanocarbon materials have received considerable attention due to their unique structure and properties, which make them promising candidate materials for use in biomedical applications. In this review, we discuss the therapeutic applications of spherical nanocarbon materials, including fullerene nanoparticles, carbon nanohorn aggregates, nanodiamonds and porous carbon nanospheres, and their toxicology in biological systems. We put special emphasis on the antitumor effects of these multifunctional nanoparticles, which operate via novel mechanisms in a highly efficient manner. The low toxicities of these spherical nanocarbon materials as well as the possible effects of shape on toxicity are discussed.

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“…However, in our experiments, DPEG-coated CNHs agglomerated after the injection into mice and were captured by macrophages or vascular endothelial cells inside the tumor blood vessels. In the previous in vitro study, smaller-sized (20-50 nm) CNHs with DPEG coating preclude their uptake by RAW 264.7 macrophage cells, 27 thus indicating a possibility that the smaller-sized CNHs with proper surface coating exit the blood vessels in tumor, which remains the challenge.…”

Section: Discussionmentioning

confidence: 94%

Ultrastructural localization of intravenously injected carbon nanohorns in tumor

Matsumura¹,

Yuge²,

Sato³

et al. 2014

IJN

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Nanocarbons have many potential medical applications. Drug delivery, diagnostic imaging, and photohyperthermia therapy, especially in the treatment of tumors, have attracted interest. For the further advancement of these application studies, the microscopic localization of nanocarbons in tumor tissues and cells is a prerequisite. In this study, carbon nanohorns (CNHs) with sizes of about 100 nm were intravenously injected into mice having subcutaneously transplanted tumors, and the CNHs in tumor tissue were observed with optical and electron microscopy. In the tumor tissue, the CNHs were found in macrophages and endothelial cells within the blood vessels. Few CNHs were found in tumor cells or in the region away from blood vessels, suggesting that, under these study conditions, the enhanced permeability of tumor blood vessels was not effective for the movement of CNHs through the vessel walls. The CNHs in normal skin tissue were similarly observed. The extravasation of CNHs was not so obvious in tumor but was easily found in normal skin, which was probably due to their vessel wall structure difference. Proper understanding of the location of CNHs in tissues is helpful in the development of the medical uses of CNHs.

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Small‐Sized Carbon Nanohorns Enabling Cellular Uptake Control

Cited by 43 publications

References 61 publications

The interactions of single-wall carbon nanohorns with polar epithelium

The interactions of single-wall carbon nanohorns with polar epithelium

Therapeutic applications of low-toxicity spherical nanocarbon materials

Ultrastructural localization of intravenously injected carbon nanohorns in tumor

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